方法

Objective To understand the relationship between effects of excite respiratory in locus coerulus complex nucleus and respiratory center of truncus encephalicus.

目的 ：探讨蓝斑复合核的呼吸兴奋效应与脑干呼吸中枢的关系。方法：本实验采用脑内核团微量注射的方法。

The MCP gene was amplified from Red-spotted Grouper Nervous Necrosis viral genome by RT-PCR. The recombinant expression vector pRSET A-MCP was constructed and transformed into BL21 (DE3) plysS to express proteins with induction in different media, at different pH, or at different temperatures.

方法］采用RT-PCR技术扩增出赤点石斑鱼神经坏死病毒MCP基因，构建重组表达载体pRSETA-MCP，以其转化大肠杆菌BL21(DE3)plysS，在不同培养基、温度、pH值条件下进行诱导表达。

Hepatocytes/isol ; collagenase digestion/methods ; hepatocytes/cytol

肝细胞/分离与提纯；胶原酶消化分离/方法；肝细胞/细胞学

Objective:To explore therapeutic method and clinical effect of severe distal radialis fracture.

目的：探讨严重桡骨远端骨折治疗方法及临床疗效。方法：47例桡骨远端骨折，男20例，女27例；年龄45~89岁，平均67岁。

Methods Four typical monitoring sites were chosen to collect TSP samples in diverse levels. 12 inorganic elements such as S, K, Ca, Ti, Cr etc. were measured directly with PIXE method. There were differences on four monitoring sites.

方法］在该地区选择了有代表性的4个监测点进行TSP分级采样，并使用质子激发X荧光发射方法直接测定样品中S、K、Ca、Ti和Cr等12种无机态元素。

Method］ Observe its influence on the pain tolerance of mouse hot plate method, on mouse ear swelling caused by xylene, on the cut-tail blood time of mouse, and on rats' hemoglutination platelet collection.

方法］观察对小鼠热板法痛阈值的影响；对二甲苯所致小鼠耳廓肿胀的影响；对小鼠断尾出血时间的影响；对大鼠凝血机能及血小板凝集性的影响。

The fluctuation of whitefly on poinsettia was studied by investigated the number of whitefly during different stages of growth period of poinsettia. The optimization comprehensive measures were studied by comparing the control effect on whitefly during different stages by different methods.

方法］调查&一品红&不同生育生长期内烟粉虱的数量，研究其消长；比较不同处理在不同时期对烟粉虱的防治效果，研究优化综合防治措施。

With 4-year-old Vitis vinifera var. Red Globe as the tested material, 4 kinds of shaping modes were set up as FI type, L type, V type and fan type and the mean leaf area, chlorophyll content, photosynthesis rate, Rubisco activity in the leaves on the middle part of grape trees with different shaping modes and their fruit quality were determined and compared.

方法］以4年生红提葡萄为试材，其整形方式设FI型、L型、V型和扇型4种，对不同整型葡萄中部叶片的平均叶面积、叶绿素含量、光合速率、Rubisco活性和果实品质进行测定与比较。

Methods］For patients 45 cases of pink 4 property Tonga with reduced ear hole buried under the treatment to seeds to chronic measles.

方法］：对45例慢性荨麻疹患者以桃红四物汤加减配合耳穴埋籽治疗。

Methods］One thousand and fifteen students from medical and non-medical colleges in Dalian were investigated by questionnaires.

方法］对大连市医学与非医学院校1015名大学生进行口腔健康知识、态度及行为的问卷调查。

The production moved off-Broadway to New York City, where she won the Joseph E. Calloway award for her performance, and was profiled in American Theater Magazine as one of ten young theater artists to watch.

生产提出过百老汇的新的纽约市，在那里她赢得了约瑟夫calloway奖，她的表现，并于异型在美国的战区杂志之一， 10战区的青年艺术家观赏。

It's a risk tradeoff, and I believe that adding test support code usually wins.

这是一个风险的权衡，我相信添加测试代码通常会占上风。