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With the SDS-PAGE preparation electrophoresis, high quality insecticidal protein was purified from a unique Bacillus thuringiensis serovar japonensis strain HBF-1, which is highly toxic to larvae of Anomala exoleta and A.

以苏云金芽孢杆菌HBF-1菌株中分离纯化的高特异性杀虫蛋白作为抗原,免疫新西兰白兔制备出多克隆抗体,其效价可达到16000。

Three different antiserum, rabbitantiserum against formalin-killed-cells of V. parahaemolyticus zj2003 (apathogenic strain isolated from diseased large yellow croaker, from Xiangshan bay,Zhejiang province), rabbit antiserum against OMP of the bacteria and large yellowcroaker convalescent antiserum were prepared for western blotting of the OMPpreparation.

以饱和硫酸铵梯度盐析法结合琼脂糖柱sephadex G200凝胶过滤法初步纯化了大黄鱼血清免疫球蛋白,再经割胶回收法得到进一步纯化的重链片段,分子量约为75kDa,免疫新西兰兔后获得兔抗大黄鱼免疫球蛋白血清,为ELISA和Western blotting检测技术奠定了基础。

Refer to the domestic and foreign literature material, to establish traumatic joint stiffness model with New Zealand big white rabbit;caused the right knee joints of the big white rabbits closed trauma with the appropriate mothod,then fixed the behind-right knee joints 4 weeks with the gypsum and the bandage;after 4 weeks, removed fixture,examined the animal blood rheology,the joint range,the skin temperature, the arthrocele extent and so on ,to authenticate whether modeling succeeded or not.

参考国内外文献资料,用新西兰大白兔建立创伤性关节僵直模型;用适当的方法造成大白兔右后膝关节闭合性损伤,用石膏绷带、绷带固定4周,4周后拆除固定。期间观察皮肤温度、关节肿胀度、关节活动度,检测动物血液流变学等指标,以验证模型建立是否成功。

Methods: Twelve healthy New Zealand male rabbits were randomly divided into methylprednisolone groups, methylprednisolone + antibiotic groupsand controlled groups. The rabbits of MG and MAG were IM methylprednisolone sodium succinate 8 mg/kg twice a week. MAG IM benzylpenicillin sodium 0.24 g twice a week yet. Increased the weight loading on the hind limbs of rabbits.

12只健康雄性新西兰白兔随机分成甲基泼尼龙组、甲基泼尼龙+抗生素组和对照组,M组和MA组均每周2次,每次肌注甲基泼尼龙琥珀酸钠 8 mg/kg,MA组每只每周2次青霉素40万 U(0.24 g)肌注,经常性增加动物后肢受力的负荷,激素注射8周后进行股骨头CT和组织病理检查,观察造模效果。

Fern of New Zealand and Australia having trailing fronds with dark green buttonlike leaflets.

新西兰和澳洲一种长有尾随复叶和墨绿色纽扣状叶片的蕨类。

The proteins were purified and used to prepare the rabbit antiserum against caprine IFN-γor IL-2,the results showed that the antibody could react with the purified proteins in Western blotting.

分别用ProBond~蛋白纯化试剂盒纯化,用所获得的重组蛋白纯化产物经三次背部皮下多点注射新西兰白兔,制备了兔抗山羊IFN-γ和兔抗山羊IL-2多克隆血清。

Right MCAO was induced in all animals by a 4-0 nylon thread with round tip inserted cranially into right internal carotid artery and maintained for 90 min. HSP70 mono-clone antibody immunocytochemistry was used to detect the changes of HSP70 expression in rat cortex at 6, 12,18,24,48 and 72h reperfusion in conjunction with histopathological analysis of rat brain.

应用HSP70单克隆抗体免疫组化染色检测大鼠再灌注6、12、18、24、48及72h脑皮质HSP70表达的变化;并与病理组织学改变进行对照研究。2.20只雄性新西兰大白兔随机分成对照组(n=10)及葛根素组(n=10),所有动物均阻闭腹主动脉肾下段20min,建立兔脊髓腰尾段缺血模型。

The rabbits underwent partial cystectomy and cystoplasty with PBAM and tissue engineering bladder.

雄性新西兰兔行膀胱大部分切除术,分别采用PBAM及构建的组织工程膀胱进行替代修补,每组6只,1、4、8、12周后进行膀胱造影并取材观察修复组织再生情况。

Materials and metheods 2.1 Experimental Animal and Model of the bone fracture New Zealand rabbits. A 3mm deossification was made in radius at distal end of mulslespronator teres as fracture healing model.

2材料与方法 2.1实验动物及建立骨折模型新西兰家兔140只,于双侧桡骨中下1/3用特制骨锯造成3mm完全骨质缺损的动物模型,不作任何固定。

Authoritative facts about the skin from the New Zealand Dermatological Society.

权威的事实,对皮肤从新西兰皮肤科社会。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。