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perforance branches of internal thoracic artery island skin flap to repair cervicofacial defects, reduce the limitation of flaps selection being of chest scar; bfgf can advance vascullar regeneration, improve microcirculation, develop flaps survival rate.

采用胸廓内动脉前穿支岛状瓣修复面颈部创伤,减少了前胸部瘢痕对皮瓣选择的限制;bfgf促进血管新生,改善创面微循环,提高了皮瓣成活率。

METHODS Neonatal HIBD models were made according Rice method, new-born rabbits wereanesthetized by aether inspiration, and their right common carotid arteries were ligated.Experimental animals were put into the hypoxic champer (8%FIO2) for two and anhalf hours. Control groups included five ligated animal which didnt undergo hypoxicmanagement.

实验方法按照Rice法制备新生兔缺氧缺血性脑损伤模型,即用乙醚吸入麻醉动物,5%盐酸利多卡因局部浸润麻醉,颈部正中切口,剥离右侧颈总动脉和迷走神经,用2号缝线将剥离出的右侧颈总动脉作永久结扎。

METHODS: NE has been shown to be a potent stimulator for neonatal rat ventricular myocyte hypertrophy in vitro. We used the activator of PPAR γ, the inhibitor of protein kinase C chelerythrine to influence protein content.3H?

在培养新生大鼠心肌细胞中,采用RSG,PKC的激动剂佛波醇酯,NE和PKC的阻断剂白屈菜季氨碱观察罗格列酮在NE和PMA诱导心肌肥大中对PKC活性和c?

Ang-1is a sort of chemotactic factor for vascular endothelial cell and a kind of promoter for angiogenesis.

Ang-1对血管内皮细胞具有明显的趋化作用,是新生血管形成的促进因子。

CONCLUTION: The compound of after-induced chondrogenetic BMSCs and SIS can engender new chondrocytes, so the repair effect is better than only transplanting SIS.

经诱导的软骨细胞-小肠黏膜下层复合物在动物体内能够形成新生软骨组织,比单纯小肠黏膜下层修复效果好。

Inflammation reaction of implantation materials reduced, degradation increased, while chondroid structures increased gradually.

皮下植入试验表明,随着植入时间的延长,炎症反应逐步减轻,材料降解增加,新生软骨样结构逐渐增多。

At 4 weeks following implantation, cells on the surface of coralline hydroxyapatite were found and connective tissues were seen in the material pores in the experimental group. Cells on the coralline hydroxyapatite were observed only in the control group. At 8 weeks, new bone formation was detected on the surface of coralline hydroxyapatite; bony tissue deposition and a few chondroid tissues were found in the pores or surrounding the pores in the experimental group. A few fibrous connective tissues were observed in the control group. At 12 weeks, abundant mature woven bone was detected on the surface of coralline hydroxyapatite; medullary cavity-like structure and vessels were found in some regions in the experimental group. No new bone or bony tissues were found in the control group.

植入材料后4周,实验组可见珊瑚羟基磷灰石表面有细胞生长,孔隙内有结缔组织长入;对照组仅见珊瑚羟基磷灰石表面有细胞生长。8周时珊瑚羟基磷灰石表面有新生骨形成,孔隙内和孔隙边缘可见骨样组织沉积和少量软骨样组织形成;对照组仅见少量纤维结缔组织长入。12周时珊瑚羟基磷灰石材料表面有较多成熟编织骨形成,部分区域可见髓腔样结构形成,并有血管长入;对照组仍未见新骨及骨样组织形成。

OBJECTIVE: To observe the inhibitory effects of hAS/293 and hES/293 cells on angiogenesis in chick chorioallantoic membrane.

目的:观察以基因工程技术构建的人血管抑素/293细胞和人内皮抑素/293细胞对鸡胚尿囊膜血管新生的抑制效应。

In chick chorioallantoic membrane assay, GLY also has inhibitory effect on angiogenesis.

而在鸡胚胎尿囊膜分析中,甘露饮也具有抑制血管新生的作用。

Objective To evaluate the value of method for quantitation of angiogenesis in chick chorioallantoic membrane using ImagePro Plus 5.0 software.

目的 应用ImagePro Plus 5.0图像处理和分析软件,研究鸡胚尿囊膜(chick chorioallantoic membrane,CAM)血管新生面积定量的新方法。

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