新生

The PAS positive layer was seen between neo-dermis and epidermis at the peripheral wound 5 weeks after wounding, while the central wound exhibited incontinuous PAS positive layer.

在第3周时，在新生表皮下方与新生真皮的部位之间见不连续的PAS阳性带，在第5周时，创面外周见连续的PAS阳性带，而在创面中央处仍不连续。

The main gluconeogenic precursor in kidney is thought to be lactate; however, less is emphasized enantiomerically. L-lactate is a glycolysis end-product, but D-lactate is formed after detoxification of methylglyoxal, which is the main source of advanced glycation end-products.

乳酸为肾脏糖质新生的主要来源，其含有一不对称碳，故具有D-、L-乳酸两种镜相异构物，而D-、L-乳酸两者之生成相当不同，L-乳酸是糖解作用之终产物，D-乳酸为体内一醣化终产物(advanced glycation end-products)─甲基乙二醛进行去毒化反应所生成，目前缺乏对乳酸镜像异构物与肾脏糖质新生间相关的探讨。

In group a,6 weeks postoperatively, a great deal of neogenetic cartilage was observed and in 12 weeks postoperatively, the shape and maturity of neogenetic cartilage were observed close to normal level in group a, which had advantages over those in group b、c.

组织学显示a组6周时生成大量新生软骨，12周时新生软骨形态与成熟度已经接近正常，较b、c两组更为优越。

The newborn neurons distributed throughout the telencephalon, mainly in the neostriatum caudale, and pars medialis;(3) Ultrastructural observation showed that newborn neurons in the NCM had the same morphologic characteristics as mature neurons and they formed synaptic connections with distant targets.

这些神经元不均匀分布在端脑的全部。新纹状体尾端中部是新生神经元的富集区；③电镜观察表明，NCM脑区中的新生神经元具有一般神经元特征并与周围神经元形成突触。

Pigment epithelium-derived factor, is a serine protease superfamily member. It has highly conservative sequence and unique molecular structure with neurotrophy, inhibiting angiogenesis,anti-tumor and a variety of functions It is now investigated as a hot spot. The function of anti-angiogenesis is particularly important.

摘要色素上皮衍生因子，是丝氨酸蛋白酶超家族成员，它具有高度保守的序列和独特的分子结构，因具有神经营养，抑制新生血管，抗肿瘤等多种功能而成为研究的热点，其抗新生血管的功能尤为重要。

Choroidal neovascularization is an important pathobiological mechanism causing a variety of choroiretinal diseases which always result in loss of vision, and is the fundamental cause of blindness, especially in age-related macular degeneration.

脉络膜新生血管膜是一系列引起视功能严重障碍的脉络膜、视网膜疾病的重要病理生物学机制，尤其在渗出性老年性黄斑变性，脉络膜新生血管膜是致盲的根本原因。

Proliferative retinopathy is a series ofretinal diseases,including diabetic retinopathy,retinopathy of prematurity,central retinal vein occlussion and retinal periphlebitis,and so on,characterized as neovascularization,recurrent vitreous hemorrhage,proliferation and even traction retinal detachment and retinal atrophy. It causes the patients serious vision damage even getting blind.

目的： 新生血管增殖性视网膜病变，是一组严重的视网膜病变，其晚期主要表现是大量视网膜新生血管形成，引起玻璃体反复出血、机化和纤维增殖，甚至发展为牵引性视网膜脱离或视网膜萎缩，导致视力严重下降进而完全失明。

It indicates that even the fracture area of the skewed group is larger, the total quantity of the poroma is not much than that of horizontal-shaped group.

新生骨痂单位面积内骨小梁面积比较，见2、4、6、8周新生骨痂中骨小梁面积横形组比斜形组大，8周时有显著性差异。

PAB, isolated from the traditional Chinese medical plant Pseudolarix kaempferi Gorden , was found to be an early pregnancy-terminating agent in 1980s.

在国家973项目的支持下，我们展开了一系列新生血管生成抑制剂的筛选和研究，并得到了几个具有较强抗新生血管生成作用的先导化合物。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。