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Robo4 is specifically expressed in tumor angiogenic sites and it is a tumor endothelial marker.

Robo4仅特异性表达于肿瘤血管新生的部位,是肿瘤血管新生标志物。

Methods the diagnosis situation of 19 cases of cryptococcal meningitis who have been confirmed from 1980 to 2004 are analysed.results there are 17 misdiagnosis cases in 19 patients, of which 7 cases are tubercular meningitis,3 are viral meningitis,2 are brain tumor,1 is cerebral edema,1 is arachnoiditis of brain and 2 are others.conclusion misdiagnosis can be discreased by promoting learning about cryptococcal meningitis, examining the doubtful patients' csf frequantly and another examinations.

对本院1980—2004年确诊的19例新生隐球菌脑膜炎病人的诊断情况进行回顾分析。结果 19例确诊前有17例被误诊,其中误诊为结核性脑膜炎7例,脑肿瘤2例,病毒性脑膜炎3例,脑水肿1例,脑蛛网膜炎1例,红斑狼疮脑病1例,其他2例。结论提高对新生隐球菌脑膜炎的认识,对可疑病人多次做脑脊液墨汁涂片检查,或其他检查,可减少误诊。

Objective To establish an animal model of asphyxial neonatal rat and compare the effects of resuscitation with 100% oxygen and resuscitation with room air on the ultrastructure and the apoptosis on the kidney of asphyxial neonatal rats,in order to provide a scientific basis for clinical choice to resuscitation.

目的 建立窒息新生大鼠动物模型,比较纯氧与空气复苏对窒息新生大鼠肾组织超微结构和细胞凋亡的影响,为临床选择使用纯氧或空气复苏提供动物实验依据。

The results Showed that twenty isolates have the following biological aspects:① Capability of forming capsule,② Growth at 37℃ within 3days,③ Forming brown colonies on Niger seed agar, CACA agar,④ Urea hydrolysis test positive within 96 hours.⑤ No blue colonies were found on CGB agar.⑥ The genus could be identified by ID32 identification kid, but the species could not.⑦ The sequence of the LSUrDNA D1/D2 region analyzed confirmed to be C. neoformans var neoformans.⑧ Serotype A and AD were found in these isolates, Serotype A was predominant(83.33%),⑨ Some biodiversities of isolates existed.

结果 20株菌皆具有以下特征:①形成英膜,②37℃3天内生长试验皆为阳性,③咖啡酸玉米琼脂和油菊籽培养基皆可以产生褐色菌落,④尿素酶试验96h皆呈阳性,⑤CGB培养基未见蓝色菌落生长,⑥ID32鉴定板可以鉴定到属,种的鉴定结果各不相同,⑦大亚基核糖体DNA的D1/D2区域序列分析证实皆为新生隐球菌的新生变种,⑧血清型仅见A和AD二型,以A型为多数(83.33%),⑨20株临床分离株随机扩增DNA多态性分析有一定的生态多样性。

Results Expressions of pDGF-BB chain and PDGFR-α mRNA were low at the pseudoglandular stage of day 18, reached the peak level at the canalicular stage of day 19 and the sacculate stage of day 21 of gestations, fell again in newborn rats, and then increased in adult rats.

结果 (1)PDGF-BB、PDGFR-α mRNA随胎龄增长而增高,高峰在19~21天,新生时表达减少,成年时又增高到接近胚胎晚期水平;PDGF-AA和PDGFR-β mRNA在胚胎晚期、新生与成年鼠肺中均有表达,不随胎龄增长而变化,肺内PDGFR-β的表达高于PDGFR-α。

Transplantation of BMSCs in neonate rats at 24 hours after HIBD has the maximal long-lasting ethological improvement, which is induced by brain damage, and the mechanism may be that early transplantation with BMSCs reduces effectively neuron necrosis and apoptosis, and is helpful for the migration and differentiation of BMSCs.

新生大鼠缺氧缺血性脑损伤后24 h接受骨髓基质细胞移植可最大程度改善脑损伤所导致的远期行为学障碍,其机制可能与早期移植可有效减轻神经元的坏死、凋亡,且有利于骨髓基质细胞的迁移、分化有关。关键词:骨髓基质细胞;细胞移植;缺氧;缺血;脑;新生大鼠

Understanding the pathogenesis of various type in exudative AMD, using the rational therapy

正确理解新生血管性疾病的发病机制,合理行使抗新生血管药物治疗

The digestion process includes the following steps: adding 0.15 % concentration collagenase II solution compounded with DMEM into blood vessel smooth muscle tissue block and acting in water bath for 1.5 hr until forming floccular tissue; cleaning with 0.02 % EDTA solution, adding 0.125 % trypsase solution and water bath vibrating to digest at 37 deg.c to obtain dispersed smooth muscle cells; terminating enzyme action with 10 % new born ox serum culture liquid; regulating cell concentration with 20 % new born ox serum culture liquid to 50,000-500,000/L, inoculation, and culturing in culture tank with 5 % CO2 and first 37 deg.c to cell fusion of 70-80 %; and subculturing conventionally.

向血管平滑肌组织块中,加入用DMEM配制的0.15%胶原酶II溶液,水浴箱中作用1.5h,至组织呈絮状,用0.02%的EDTA清洗,然后,加入0.125%胰蛋白酶溶液,于37℃水浴振荡消化,即可得到分散的平滑肌细胞,用10%新生牛血清的培养液终止酶的作用。加入含20%新生牛血清的培养液,调节细胞密度为5×104~5×105个/L,接种,放入5%CO2、37℃培养箱中培养。当细胞达到70%~80%融合时即可按常规方法传代。

In addition, NO, calcium and adhesion molecules mediate the processes of angiogenesis. Thus, we chose fluorene, one of PAHs which contain three bezene rings, to study the effect of PAHs on angiogenesis in HUVECs.

而许多文献也指出一氧化氮、钙离子浓度的变化及黏附因分子的表现在血管新生中扮演一种媒介的角色,所以在此我们选取具三苯环的芴来探讨多环芳香烃对於血管新生方面的影响。

Results: No fiber layer and inflammatory tissues around any inserted implants were founded. New-grown osteoid tissue appeared faster in Bio-Ti implants than pure titanium and matured bone tissues comprise bone trabecula and Haversian canals appeared in 6 weeks, while at least 12 weeks needed to form matured bone tissue in control groups.

结果:所有种植体周围没有发现明显炎症组织或形成纤维层,Bio-Ti种植体组新生类骨组织生长较快,并于6w时已形成含有骨小梁及哈弗氏小管结构的较成熟骨组织,对照组12w时新生骨组织基本成熟。

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