新生

Methods To establish asphyxia models of low degree,moderate degree and severe degree of SD,collected samples of gastric mucous membranes,and by using electroscope and DNA electrophoresis to examine cell appotosis;by using RNA dot blot and immunohistochemistry to examine expressed levels of fas gene and FAS protein.

方法用20只新生SD大鼠建立轻、中、重度窒息模型，采集胃粘膜标本，分别进行电镜、DNA电泳检测细胞凋亡是否存在；采用RNA打点杂交及免疫组化技术检测凋亡基因窒息模型。

It is only seen in the earlier period of embryogenesis and organ formation.

血管新生是指在已经存在的血管的基础上以出芽的方式形成新的微血管的过程，可见于胚胎发生期以及出生后。

However, I believe in emergent democracy and the importance of celebrating the whole community.

但是，我也坚信民主的新生和展示整个团体的重要性。

Emmie's baby has been born and is dying and must be baptized .

埃米的新生婴儿快要死了，可是还得他施洗礼。

Objective To investigate the prevention and treatment effect of nerve growth factor on hypoxic-ischemic encephalopathy.

目的 探讨神经生长因子对新生大鼠缺氧缺血性脑病的影响。

Methods: 98 cases of neonatal hypoxic-ischemic encephalopathy were randomly divided into two groups.

选择新生缺氧缺血性脑病患儿98例，随机分为两组。

Objective To evaluate protective effect of nerve growth factor on hypoxic-ischemic encephalopathy in newborn rats.

目的 观察神经生长因子对新生大鼠缺氧缺血性脑损伤的保护作用。

Methods Eukaryotic expression plasmid pCMVCD was constructed , and identified by re2 striction endoenzyme digestion. CD gene was transfected into NSCs from new2born Wistar rats using Lipofec2tamine2000. Positive clones (named NSCs/ CD cells) were screened by G418 presence. 52Fluorocytosine (52FC) ad2ministration of different concentrations were incubated with NSCs/ CD cells. NSCs/ CD cells viability ratios were mea2 sured by MTT assay.

通过构建真核表达质粒pCMVCD ，限制性内切酶消化鉴定后，采用Lipofectamine 2000 脂质体介导法转染新生大鼠室管膜下区神经干细胞(Neural stemcells ， NSCs)，G418 筛选阳性克隆，加入不同浓度的52氟胞嘧啶(52Flourocytosine ， 52FC)，MTT比色法测定NSCs的生存率。

It endures, with leaves ever new, always with more food for us to eat.

它忍受着，树叶不断的新生，总是让我们有更多的东西可以吃。

Une vie à me cacher, et puis libre enfin

我摆饰餐桌，明日又是新生

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。