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Result 1 Magnetic nanoparticles, magnetic nanoparticles modified with antisense oligodeoxynucleotide of human telomerase reverse transcriptase induced HL-60 tumor cells to apoptosis, we could see typical morphologic change of apoptosis cells: karyopyknosis, chromation"s condensing and aggregation in nuclear, forming crescent-shaped or annulus structures to lean on edge of cell nucleus"s membrane and posing apoptosis body by Atomic Force Microscope, Fluorescence microscope, transmission electron Microscope 2 There was a significant difference compared with control group(p.01), inhibition ratio had significant positive correlation with medication dosage and time ;during 0.8-8μM dosage amplitude, inhibition ratio accrescenced by dosages increasing. However, the inhibition ratio would decrease when dosage over 8-80μM.

结果 1 磁性纳米粒子、修饰有端粒酶反义寡核苷酸的磁性纳米粒子诱导HL-60细胞发生凋亡,原子力显微镜、光学显微镜、荧光显微镜和透射电镜下均观察到HL-60细胞呈现典型的凋亡细胞的形态变化:细胞核固缩,核内染色质浓缩、凝聚、形成新月形或环状结构紧靠在细胞核膜边缘,并形成凋亡小体。2 磁性纳米粒子、修饰有端粒酶反义寡聚脱氧核苷酸的磁性纳米粒子对HL-60肿瘤细胞的生长和增殖有明显的抑制作用,与对照组相比有显著性差异(p<0.01),在剂量为0.8-8μmol/L范围内,抑制率随剂量的增加而增加,当剂量超过8μmol/L时,抑制率反而下降;3 磁性纳米粒子、修饰有端粒酶反义寡聚脱氧核苷酸的磁性纳米粒子可增强p53基因的表达活性,引起DNA降解损伤,反向调节细胞周期活动,促使细胞从G0期进入G1期,抑制肿瘤细胞的生长。4 修饰有端粒酶反义寡聚脱氧核苷酸的量子点能通过内吞作用进入HL-60肿瘤细胞的细胞核,可以在细胞内进行定位和促进HL-60肿瘤细胞的凋亡。

Under electron microscope, tumor cell emerged karyopyknosis, new-moon shaped or circular, nucleus membrane was clesr, endoplasmic reticulum was dilated, and mitochondrion was swollen. A lot of apoptosis cells emerged in the tumor foci under light microscope.

光镜下显示肿瘤灶中出现许多凋亡细胞;肿瘤组织电镜观察,瘤细胞核染色质浓集成块,在核膜内呈新月形或环形排列,核膜清楚,瘤细胞内质网扩张,线粒体肿胀。

In recent years, there were a few correlative study on production of flocculants and laccase by Curvularia lunata.

近年来也有少量关于新月弯孢霉生产絮凝剂和漆酶的报道。

Corolla blue or white; tube usually shorter than calyx; throat appendages 4, lunate or trapeziform; lobes 5, spreading, overlapping.

花冠蓝色的或白色;通常的筒部短于花萼;喉附属物4,新月形或;裂片5,平展,重叠。

To the ptotic breast company with macromastia,using the two annular incisions with reduction mammoplasty.

对正常体积的乳房松垂,选择新月形或双环形切口,将乳腺上缘固定于相应的胸壁上。

Fig.10 Showing K562 cell line treated with CA for 24h:an apoptotic cell with condensed chromatin marginated in crescents around the nuclear envelope.

图10 示2μm/L CA作用24h,加药组中1个凋亡的K562细胞,可见其染色质靠核膜边集,呈新月

Fig.13 showing K562 cell line treated with NCTD for 24h:an apoptotic cell with condensed chromatin marginated in crescents around the nuclear envelope.

图13 示10mg/L NCTD作用24h,加药组中1个凋亡的K562细胞,可见其染色质靠核膜边集,呈新月形,胞浆浓缩

Premiere has been following him from the very start and met with him this summer on the set of New Moon, the second film of the series, in the medieval city of Montepulciano in Italy.

首映杂志从一开始就关注他,并且今年夏天在拍摄系列的第二部新月的意大利中世纪古城Montepulciano见了他。

The Esterase, Catalase, PPO isoenzyme and soluble mycoprotein patterns of 43 isolates were analyzed by polyacrylamide gel electrophoresis.

利用聚丙烯酰胺凝胶电泳技术对43个新月弯孢分离物进行了酯酶等三种同工酶及可溶性蛋白的分析。

Pathogenieity and virulence of the isolates were assessed; Soluble mycoprotein and esterase etc isoenzynie patterns of 43 isolates were analyzed; Genetic diversity among 40 isolates of Curvularia lunata was evaluated by random amplified polymorphic DNA fingerprint.

通过对43个新月弯孢分离物的3种同工酶及可溶性蛋白分析和40个分离物的RAPD指纹图谱分析,表明玉米弯孢菌叶斑病病原群体存在遗传多样性。

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