新

Vehicle breaks down the old one-unit administerial system and puts political activity in a new framework, new territory and new process.

载体创制格局打破了原来行政体系的单中心位置，使政治活动在一个新框架、新领域与新方式下进行。

This papper figures that the significant value that "three representative" momentous thought on socialistic affair of 21th century as bellow: its theoretic innvation on scientific socialism brought accurate guidance for socialistic affair development of 21th century;its new theory that based on deepening the knowledges about China's characteristics socialism has the meaning for generally guide socialistic affair of 21th century;it disclosed the factor that pushing forward the development in socialistic affa...

&三个代表&重要思想对21世纪社会主义事业的重大价值在于：它对科学社会主义的理论创新给21世纪社会主义事业的发展以正确指导；它在深化对中国特色社会主义认识基础上所作出的新的理论结论对21世纪社会主义事业具有普遍指导意义；它揭示了21世纪发展社会主义事业的动力问题；它指出了21世纪多模式社会主义新的共同本质规定性；它揭示了21世纪社会主义发展的新特点。

Written by Brian archangel Bendis, the illustrator behindhand the Avengers Disassembled storyline, the program depicts a assemble of superheroes that modify a newborn aggroup of Avengers, regularly referred to in the program as the "New Avengers", and mass 2006, The New New Avengers.

由布莱恩·迈克尔·本迪斯撰稿，作者延续了《复仇者解散》的故事线。该系列描写了一群英雄集合组成了一个新的复仇者战队，也就是经常提到的新系列——&新复仇者&。

The presented papers in the XXIX IULTCS Congress and 103rd ALCA Annual Meeting were reviewed The papers covered the following field tanning mechanism treatment of tannery effluents environmental regulation biodegradation of leather and leather chemicals utilization of leather waste clean technology of leather making preservation of raw hides and microbial protection of leather novel leather process and materials physical and mechanical properties of leather new analytical and detecting equipments and method .

本次会议论文主要涉及：皮革鞣制机理的研究，皮革行业的污染治理，皮革行业环境保护政策及动向，皮革及其化学品的生物降解，胶原及制革废弃物的资源化利用，制革清洁生产技术，生皮的清洁化防腐保存和皮革的抗菌防霉，制革新工艺和新材料，皮革的物理机械性能及新的皮革分析检测设备和方法等。

The presented papers in the XXIX IULTCS Congress and ALCA Annual Meeting were reviewed The papers covered the following field tanning mechanism treatment of tannery effluents environmental regulation biodegradation of leather and leather chemicals utilization of leather waste clean technology of leather making preservation of raw hides and microbial protection of leather novel leather process and materials physical and mechanical properties of leather new analytical and detecting equipments and method .

本次会议论文主要涉及：皮革鞣制机理的研究，皮革行业的污染治理，皮革行业环境保护政策及动向，皮革及其化学品的生物降解，胶原及制革废弃物的资源化利用，制革清洁生产技术，生皮的清洁化防腐保存和皮革的抗菌防霉，制革新工艺和新材料，皮革的物理机械性能及新的皮革分析检测设备和方法等。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

An arborescent generating method is used to create a new system from the existing system, that is, 'growing' the new building from the old as new branches grow on a tree.

一个木本生成方法用于创建一个新的系统从现有的系统，也就是不断增长的'新大楼从旧如新的分支机构上生长的树。

We wield the question two step analysis law , make the new aristogenesis number form , build the new model , lay on new being in hospital a personnel again and then, to the circumstance not arranging operation for on Saturday , on Sunday.

然后，对于周六、周日不安排手术的情形，我们运用问题二的层次分析法，作出新的优生数表，建立新的模型，重新安排新的住院人员。

I. Background, intention and the scope of the studying With the development of information and communication technology, especially the development of networking technology, the consumers' way of shopping has also changed (traditional aspectant trading → purchasing for commodities and services at any time and anywhere, surpassing time and space → buying commodities in the virtual world).

一、研究背景，目的和研究范围随着信息通信技术的发展，特别是网络技术的发展在人们的日常生活与企业活动中有了很多变化。消费者的购买方式也变化了。（传统的面对面方式→超越时间与空间随时随地可以购买商品-在虚拟世界中购买商品）。这样的发展环境下电子交易成为了21世纪世界交易的新趋向。随着通信技术与电脑技术的飞跃发展出现了没有时空限制的新交易类型，而这种类型的新交易实现了交易的多样化，大量化，信用化，国际化等。

On the other hand, relapplied science directly ates to how the common law of theoretical science can be applied to actual life things,and to enhance human control on the environment,resulting into the development of new technologies,new methods and new machinery.

另一方面，应用科学则直接关系到如何把理论科学的通用定律应用到生活的实际事物中去，应用到加强人类对环境的控制上去，从而导致新技术、新方法和新机械的发展。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。