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Results: The autofluorescence of M. furfur and M. japonica standard trainsrespectively in 4th and 6th day are stronger than that in other days, andautofluorescence of colonies become weaker in an hour. Theautofluorescence of Malassezia colonies put in 3% glutaraldehyde, pH4.0HCL, 1280μg/mL and 64μg/mL fluconazol solution, 0.3% methylene blueseparately get stronger, and that in 10% KOH get stronger at beginning thenbecome weaker 2 days later. C. albicans, S. schenchii, T. rubrum, T.tonsurans, A. terreus, A. fumigatus cultured on SDA all haveautofluorescence, and which are weaker than that of Malassezia.

结果:糠秕马拉色菌和日本马拉色菌2株标准株的自发荧光强度分别在培养第4和8天时最强,菌落的自发荧光在1小时内逐渐减弱;3%戊二醛溶液、pH4.0盐酸溶液、1280μg/mL和64μg/mL氟康唑溶液、3%美蓝溶液处理后马拉色菌自发荧光都增强,10%氢氧化钾溶液中的马拉色菌在开始时荧光增强,2天后减弱;白念珠菌、申克孢子丝菌、红色毛癣菌、断发毛癣菌、土曲霉、烟曲霉等在培养条件下有自发荧光,但都较马拉色菌弱。

To explore the possibility of early and rapid diagnosis of mycosisby detecting the fungal autofluorescence. Methods: To culture M. furfur and M. japonica standard strains on Dixonmedium at 32℃, and to study the autofluorescence of colonies in 2nd, 4th, 6th, 8th, 10th day under Laser Scanning Confocal Microscopy (LeicaTCS-SP2 LSCM). To study the variation of autofluorescence whenMalassezia colonies were put in 3% glutaraldehyde, pH4.0 HCL, 10% KOH, fluconazol solution, 0.3% methylene blue separately. To detect theautofluorescence of C. albicans, S. schenchii, T. rubrum, T. tonsurans, A.terreus, A. fumigatus cultured on Sabouraud dextrose agar.

选用糠秕马拉色菌和日本马拉色菌2株标准菌,接种在Dixon培养基32℃培养,分别在培养的第2、4、6、8、10天挑取菌落放在TCS-SP2型激光扫描共聚焦显微镜(Leica TCS-SP2 LSCM)下观察、测定其自发荧光;分别用3%戊二醛溶液、pH4.0盐酸溶液、10%氢氧化钾溶液、1280μg/mL和64μg/mL氟康唑溶液、0.3%美蓝溶液处理后观察马拉色菌自发荧光的变化;选择白念珠菌、申克孢子丝菌、红色毛癣菌、断发毛癣菌、土曲霉、烟曲霉等用沙堡葡萄糖琼脂培养后观察其自发荧光的特点。

Oh and all that stubble on my face.

表示惊讶和我的脸上所有的那个断株

This thesis studies each structural layer's feature:(1) structural layer of basement:it is a Paleozoic folded basement,whose tectonic deformation and magma intrusion is fiercely,and it is affected by the early Hercynian tectonic movement and related to the underthrust and closure (C1) of Zhungaer ocean basin and the areal pressing of continents (C2-3);(2) structural layer of Permian:due to the regional extending and fault depression role in mid-Hercynian,it occurred to the combinations of structure and magma in Santanghu basin and it's periphery area,such as A-granite's stock,batholiths and diabase's dike.

二叠系构造层:受中海西区域性伸展断陷作用的影响,盆地及其周缘地区发育与伸展构造有关的构造-岩浆组合,如呈岩株、岩基状侵位的A型花岗岩以及呈岩墙状侵位的辉绿岩等。

The single micronucleus and multi-micronucleus were observed at the interphase. The chromosome aberrational cells including chromosome fragment, lagging chromosome, chromosome bridge and circular chromosome were found during the mitosis. RAPD analysis of seedling genomic DNA variation in M 2 generation of three mutants showed their DNA sequences had changed. The result confirmed that the implantation of 7Li +3 ions could induce genetic mutation in wheat.

对幼苗根尖细胞学研究观察,获得单桥、双桥、桥断裂、落后染色体、三级、断片和微核等畸变类型;对已获得的小麦突变株,分别进行M2 代DNA分子的RAPD分析,结果发现DNA均发生变异,证明7Li+ 3 注入通过内靶核反应能够诱发小麦在分子水平上产生遗传变异。

Based on surface and subsurface geological data, combined with achievements about the west Kunlun orogen, Tibet, Tarim basin and Altun tectonic zone, it is analysed that geometry and kinematics of the west Kunlun fold-thrust-belt between Sangzhu country in the west and Cele county in the east.

本文通过对研究区地面地质资料和地下地质资料的分析,并结合西昆仑造山带、青藏高原、塔里木盆地和阿尔金构造带研究的新进展,对西昆仑北缘冲断带桑株—策勒段的构造形态和演化历史作了深入的剖析与探讨,取得了一些新认识。

One day a stubby little boy said,"last night moon went with me all they way When I went out to walk."

有一天,一个断株一般的小男孩说,&昨晚月亮和我去全部他们方法当我外出走的时候。&

Sometimes many of these hair stubs fall out attached to the separated crusts; sometimes they persist longer.

有时许多这些头发断株解散对被分开的外壳附件;有时他们坚持更久。

Steering arms and a track tod link two stub axles together for swivelling them by a steering wheel about the king pins.

掌舵双臂和轨道狐藉着一个方向盘为 swivelling 一起联编二根断株轮轴他们有关国王大头针的事。

From there my mother contemplated the broken pots of begonias, the rotted stubble, the trunk of the jasmine plant eaten away by ants, and she recovered her breath.

我母亲在那里对着破了的秋海棠花盆,腐烂了的断株和被蚂蚁们吞噬着的茉莉树干凝视良久,呼吸渐渐平缓下来。

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