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斑马鱼

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This study used Danio rerio, which was a sensitive fish to environmental toxin and Hypophthalmichthysmolitrix, which had strong absorbability to environmental toxin to be the eucoen, and behavior index of these two fishes was used to monitor the existence of microcystins and malachite green.

本研究用人工饲养,对环境毒素敏感的斑马鱼和对环境毒素有较强吸收能力的鲢鱼作为指示生物,检测其行为对水体中微囊藻毒素和孔雀石绿的反应。

To study the sedimentation coefficient of ribosomal RNA from Saccharomyces cerevisiae,the enzymolysis method and the liquid nitrogen grounding method combined with Trizol Reagent were used for total RNA extraction from Saccharomyces cerevisiae.RNA extraction in Mus musculus Kunming and Danio rerio,was also involved in,which was set as control.

为研究酿酒酵母菌核糖体RNA的沉降系数,用酶解法和液氮研磨法裂解酿酒酵母菌的细胞壁,Trizol Reagent提取其总RNA,同时提取小白鼠和斑马鱼的总RNA进行比较。

The present study wastherefore designed to examine the molecular toxic effect of MC-LR on bighead carpAristichthys nobilis and zebrafish Danio rerio by using real-time polymerase chainreaction, molecular cloning and immunoblotting.

本研究以斑马鱼和鳙鱼为对象,采用实时荧光定量PCR、分子克隆和免疫印迹技术在分子水平上揭示了MC-LR对鱼类的毒理效应。

RESULTS: The polymerase chain reaction product was in concordance with target fragment. The analysis of the gene sequence proved it is the ALR gene. The overall length of the ALR gene was 378 bp, it was highly conservative in many species, for instance the Homo sapiens, the Mus musculus, the Bos Taurus and the Danio rerio.

结果:聚合酶链反应扩增结果与目的片段位置一致,测序结果表明为肝再生增强因子mRNA,基因全长378 bp,在人、牛、小鼠、斑马鱼等物种中高度保守。

Further bioinformatical analysis indicated that the three Y-box genes of lamprey, i.e., Lyb1, Lyb2, and Lyb3, encoded 331, 181, and 171 amino acids, respectively. The three amino acid sequences were homologically aligned with those from Ciona intestinalis, Danio rerio, Oryzias latipes, Takifugu rubripes, and Mus musculus by DNAMAN software.

采用DNAMAN软件,将七鳃鳗的3个Y-box蛋白氨基酸序列与海鞘、斑马鱼、清鳉、河豚鱼和小鼠Y-box蛋白进行同源性分析,结果显示他们共有一个保守的冷休克结构域,其中包含两个RNA结合基序RNP-1和RNP-2,表明获得的Lyb基因属于Y-box 基因家族成员。

The deduced amino acid sequence shared 66.5%-90.8% identity with other fishes compared to Sparus aurata, Oncorhymchus keta, Cyprinus carpio and Danio rerio.

编码的蛋白序列含有FAD全部的特征结构区,包括3个组氨酸簇、2个跨膜区和1个细胞色素b5结构域,与金头鲷、大麻哈鱼、鲤鱼和斑马鱼FAD的氨基酸同源性为66.5%—90.8%。

The deduced amino acid sequence of Carassius auratus gibelio had high similarity with that of Carassiu auratus,Danio rerio,Ictalurus punctatus,Oncorhynchus mykiss and Takifugu rubripes. The similarities were 92%, 88%, 79%, 75% and 72%,respectively. The full-length TLR3 of Carassius auratus gibelio contained a signal peptide in the first 21 amino acid residues, 15 leucine-rich regions from 50th to 695th amino acid residue, a transmembrane region from 704th to 726th residue, a Toll/IL-1R homologous region from 754th to 897th residue.

经序列同源性分析显示,其编码的氨基酸序列与金鱼Carassius auratus]、斑马鱼、斑点叉尾鮰、虹鳟和红鳍东方鲀有较高的相似性,其相似性分别为92%、88%、79%、75%和72%,说明TLR3在长期的进化中具有较高保守性。

In recent years, a research team at the University of Washington in Seattle has been working on finding a way to resolve that problem in experiments involving the zebrafish, a common aquarium denizen.

近年来,华盛顿大学西雅图分校的一个研究团队一直在对一种水族馆里常见的观赏鱼类──斑马鱼进行研究,试图解决人类听力丧失的问题。

Zebrafish is one of the most important model species for developmental biology study in vertebrate.

斑马鱼是研究脊椎动物发育生物学的重要模式物种之一。

At approximately 24 hours post-fertilization, fez expression is mostly confined to the telencephalon and the anterior-ventral region of the diencephalon.

受精后24小时的斑马鱼胚胎中,Fez的表达范围主要分布在胚胎的端脑和间脑的腹侧前端。

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