斑马鱼

At 24 h, Ang1 was expressed mainly in the lens, optic cup, mesencephalon, ventral part of hindbrain, and myotomes in the somites, while zfTie2 was mainly expressed in intersomitic vessels including dorsal aorta and axial veins, and sprouting vessels from the dorsal aorta in the trunk.

采用多聚酶链式反应，扩增了长约280bp的斑马鱼血管发生素1 cDNA部分序列，作为探针，以原位杂交法测定了血管内皮细胞生长因子和血管发生素1及其相应受体flk1和Tie2的表达。

To address this issue, we microinjected Morpholino Oligonucleotides, which acts as a specific translational inhibitor, into 1-cell stage zebrafish embyos to knockdown the expression of FoxD5. Results showed that the newly forming somites of the FoxD5-MO-injected embryos were malformed both in the stage when the somitogenesis was completed (24-hpf) and in the stage when the somitogenesis was in progress (14-hpf).

在本篇研究中，为了了解FoxD5在体节生成时的功能，利用专一抑制基因转译之Morpholino Oligonucletides对1-cell时期之斑马鱼胚胎进行显微注射以抑制FoxD5的转译，并在体节发育已完成之24 hpf时、及体节仍在形成之14 hpf (约12-somite stage)时分别观察FoxD5-MO注射个体(FoxD5 morphants)，发现新生成之体节处有产生体节发育畸形的现象。

SINEs insertion can be stably delivered to descendants, thereby, SINEs can provide the reliable evolutionary information, and used as molecular markers for phylogeny events within 50Mys, such as the levels of family and order.Cyprinidae, the largest fish family, comprises approximately 210 recognized genera and 2010 species.

鲤科鱼类是世界上现生鱼类中最大的一个科，约有210属2010个种，但有关鲤科鱼类中SINEs的研究却很少，目前相关的报道仅限于斑马鱼基因组内；而有关利用SINEs插入位点信息进行鲤科鱼类系统进化的研究更没有报道。

The construction of expressed plasmid in prokaryote not only obtained abundant protein but done research further for its separation, purification and biological activity.

构建斑马鱼GnRH原核表达质粒不仅获得大量的GnRH蛋白，还可对该蛋白做进一步分离纯化以及生物活性方面的研究。

Like lizards, newts, and frogs, a zebrafish can replace surprisingly complex body parts.

像蜥蜴，蝾螈、青蛙，竟然斑马鱼可代替复杂部位。

To elucidate the mechanisms regulating TSHb expression, we isolated a 5.1-kb fragment of the red-spotted grouper 5'-flanking region by Genome walking .1.8kb of this upstream region was used to produce GFP reporter construct (pBK-TOL2) for analysis of tissue-specific expression in zebrafish embryos.

将起始密码子上游1864bp的启动子片段顺向克隆到pBK-TOL2载体中， pTSHb-TOL2重组质粒与转座酶mRNA共注射斑马鱼一细胞期受精卵，荧光显微镜观察发现，TSHb启动子驱动GFP在原始生殖细胞和垂体中表达。

Employing microarray analysis, we found that 14 apoptosis related genes including caspase-2 changed their expressions after pentachlorophenol exposure to zebrafish embryos.

应用基因芯片技术研究发现，暴露于五氯酚的斑马鱼胚胎中有14个涉及细胞凋亡行为的基因表达发生了显著改变。

As noted in the highlighted box on Sheet "Cost Calcs" the spreadsheet calculates what is refers to as the "'Calculated Sel/Conv Balance Ratio".

正如在高亮显示的盒子放在纸&的成本计算电子Calcs&是指为&计算摘录/斑马鱼平衡率&。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。