斑马鱼

Therefore, the defect of convergence movement may affect cell determination during gastrulation in cyp11a1 deficient embryos.

因此cyp11a1表现下降的斑马鱼胚胎会有背部组织的缩小很可能是由於聚合移动速度下降所致。

Full-length cDNA of Gibel carp pou2 gene was cloned from gastrula SMART cDNA library through RACE-PCR. It consists of 2421 bp, with an ORF of 1416 hp encoding a protein of 471 aa. The complete amino acid sequence shares 91.0% identity with zebrafish pou2 gene product.

用RACE-PCR方法从原肠期SMART文库中扩增到银鲫pou2基因的全长cDNA，其全长为2421bp，开放阅读框为1416bp，编码471个氨基酸，与斑马鱼pou2基因的氨基酸序列一致性高达91.0%。

Importantly, recent analyses8 have begun to reveal new relationships between early vertebrates, in which acanthodians and placoderms are scattered among the early divisions of gnathostome evolution; acanthodians, in particular, are cropping up on chondrichthyan and osteichthyan stem groups.

有颌类包括硬骨鱼类以及软骨鱼类。软骨鱼类仅占有颌类物种数的2%，其余98%的有颌类都属于硬骨鱼类。在硬骨鱼类中，大约一半是辐鳍鱼类，剩余的统称肉鳍鱼类。辐鳍鱼类包括从斑马鱼到多鳍鱼的大约2万8千个种。

Hoxall genes from 16 vertebrates were analyzed, containing 10 cloned Hoxall genes mentioned above and 6 Hoxall genes from GenBank, Homo sapiens, Mus musculus, Bos taurus, Polyodon spathula, Heterodontus francisci, and Oryzias latipes, in representatives of the three major gnathostome lineages: cartilaginous fish, ray-finned fish and lobe-finned fish.

克隆的基因与从GenBank中获取的人、小家鼠、黄牛、匙吻白鲟、角虎鲨Hoxa11基因及青鳉Hoxa11a基因进行比较分析，这些动物代表了有颌类的三个谱系：软骨鱼类谱系、辐鳍鱼类谱系(斑马鱼、大黄鱼、花鲈、罗非鱼、红鳍东方鲀、乌鳢、青鳉和匙吻白鲟)和肉鳍鱼类谱系(矛尾鱼、热带爪蟾、绿背树蜥、家鸡、人、小家鼠和黄牛)。

At the one-cell stage, each embryo was injected with gradient doses of 2-8ng MO. The control morpholino, was purchased from Gene-Tools.To test the knockdown effectiveness of the morpholino, the tbx2-EGFP (enhanced green fluorescent protein) construct was generated. And coinjection of morpholino with mRNA tests its specificity of the morpholino. Overexpression of tbx2 via mRNA microinjection helps us understand gaining function of tbx2. Results The tbx2-EGFP construct tests the knockdown effectiveness of the morpholino. Zebrafish embryos lacking tbx2 function have defects in cardiac contractility, rhythm and morphology in a dose-dependent manner. The embryos with c-MO are normal in development. Coinjection of morpholino with mRNA rescue the phenotype of tbx2 knocking down. The phenotypes include pericardial edema, hypogenetic ventricle, dilation of atria, arrhythmia, bradycardia, asystole, abnormal atrioventricular canal, aberrant valve and blood regurgitation.

结果荧光蛋白融合标记实验验证了tbx2-MO可以很好得阻抑斑马鱼胚胎tbx2的表达，tbx2-MO组胚胎在心脏收缩性、节律和形态学发生上出现了异常，心脏畸形随着注射剂量的增加而出现比较一致的表型，而c-MO组胚胎没有出现明显的畸形；tbx2-mRNA和tbx2-MO的共注射减少了tbx2-MO胚胎心脏畸形的发生率及减轻了心脏畸形的程度，验证了所设计合成的tbx2-MO对tbx2基因的抑制作用具有特异性；tbx2-MO组胚胎心脏缺陷包括心室发育不良、心房扩张、房室管和瓣膜异常以及心率缓慢、心律不齐、心脏停搏、血液返流等，并根据心脏发育异常的程度分为轻、中、重度畸形组tbx2-mRNA基因过表达胚胎没有见到心腔的形成。

The use of retrovirus-mediated insertional mutagenesis in zebrafish has led to the mutation and rapid identification of hundreds of genes required for embryonic development and cell growth.

运用反转录病毒介导的插入突变技术，在脊椎动物斑马鱼中已经获得了许多影响胚胎发育和细胞生长过程的突变体，并找到了对应的基因。

The results of RT-PCR showed that zebrafish treated with TAA had adipogenetic and lipogenetic genes up-regulated on the third and 5th day, After injected pLF2.8-zfPPARγ 5-day postfertilization, treaded TAA 10 days.

由我们实验室发表的报告中，指出硫乙胺处理斑马鱼稚鱼七天，将造成脂肪肝炎。

There has been a research indicating that geminin overexpression or cdt1 knockdown decreased the number of mitotically active cells and this effect depended on direct interaction between Geminin and Cdt1 .This balanced interaction of Geminin and Cdt1 is critical for the cell-cycle regulation.

在斑马鱼中研究发现，过量表达Geminin或者降低Gdt1的表达能减少有丝分裂的细胞数量，并且这种对细胞周期的效应直接取决于Geminin-Cdt1的相互作用，总之，Geminin和Cdt1的相互作用对细胞周期的调节非常重要。

The STR method is simple to operate,and it deserves to further study and lay the foundation for genetic mornitoring standard of outbred stock zebrafish.

结论鉴于该方法操作简便，通过进一步研究，可为封闭群斑马鱼的遗传质量评价打下基础。

Gpd, Ce, Mod, Gpi and ES can be used as the biochemical genetic markers for evaluation of different outbred stock zebratlsh.

Gpd、Ce、Mod、Gpi、ES可作为3种封闭群斑马鱼群间评价的生化标记，通过进一步研究，可建立封闭群斑马鱼的遗传标准。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。