斑马鱼

The genetic inactivation of eggs is the first key stage of androgenesis in fish. In order to observe the effect of UV irradiation for the inactivation of female nucleus of zebrafish the eggs were irradiated by UV with solution-free, pure water, Ringer's solution and Cortland solution. The results showed that abnormal fry were obtained in all groups and haploids were validated. But irradiation with solution was confirmed it could not inactive fully female nucleus and the fertilization rate of the eggs irradiated with pure water was very low.

雌核灭活是雄核发育的第一个关键步骤，为研究紫外线对斑马鱼卵的雌核灭活效果，将斑马鱼的卵采用干法或置于纯水、Ringer氏溶液和Cortland鱼用生理盐水进行紫外线照射，结果表明，各种方法中均得到畸形鱼苗，并经染色体检查大多为单倍体；四种方法中在Cortland鱼用生理盐水中灭活效果最好，120 mJ/cm2紫外线可以使雌核完全灭活；干法处理不能达到雌核完全灭活的目的，在纯水中灭活后的卵子受精率较低。

The results on the effects of micronucleus in erythrocytes of Danio rerio induced by bensulfuron-methyl at different times and different concentrations showed that the MN rate of control group was 0.0103%, the highest MN rate of experimental group reached to 0.372%, it also indicated that bensulfuron-methyl herbicide had genetics toxicity to Danio rerio.

不同处理浓度和染毒时间对斑马鱼红细胞微核形成的影响试验表明，对照组红细胞微核细胞率为0.010 3%，处理组红细胞微核细胞率最高达0.372%，说明除草剂苄嘧磺隆对斑马鱼具有遗传毒性作用。

The identity between pmDmrt2 and the Dmrt2 of Danio rerio, Xenopus Laevis , Gallus gallus , Mus musculu ,Homo sapiens is 95%, between pmDmrt3 and the Dmrt3 of Danio rerio, Oryzias latipes, Gallus gallus, Rattus norvegicus,Homo sapiens is 85%, and 97% between pmDmrt4 and the Dmrt4 of Oryzias latipes and Takifugurubripes. Our results further reveal the evolutionary conservation of the DM domain gene family in both invertebrates and vertebrates.

序列分析表明，pmDmrt2编码的氨基酸序列与斑马鱼、爪蟾、红原鸡、家鼠、人等的Dmrt2的一致性为95 %；pmDmrt3编码的氨基酸序列与斑马鱼、青鳉、红原鸡、家鼠、人的Dmrt3的一致性为85%；pmDmrt4编码的氨基酸序列与青鳉、红鳍多纪鲀的Dmrt4一致性高达97%，进一步证明该基因家族在无脊椎动物和脊椎动物都具有高度的进化保守性。

Based on the above results, we can conclude that Mist1 functions in an evolutionary conserved way as a key transcriptional regulator specific for exocrine pancreas development in zebrafish. In addition, it is involved in the embryonic CNS development, and even earlier initial period of development.

根据以上结果可以得出结论，Mist1作为转录因子，以一种进化保守的方式特异性调控斑马鱼外分泌胰腺的发育；另外，它还在斑马鱼胚胎早期发育过程中参与了中枢神经系统的发育，以及更早的发育起始过程。

Two gene fragments of DEN-1(GenBank accession number DQ886390)and DEN-2(GenBank accession number DQ886391),differentially expressed between the ocular skin tissues of normal and albino turbot,were isolated by mRNA differential display and sequenced.It was confirmed by RT-PCR method that the expression levels of both DEN-1 and DEN-2 were down-regulated in the ocular skin tissue of albino turbot.The sequence homology search of DEN-1 revealed high sequence homologies with the UGGT genes of zebrafish and cattle,and the Ugcgl1 gene of Norway rat.High sequence homologies of DEN-2 were observed with the eya4 gene(the eye absent homolog 4)from red jungle fowl,zebrafish,human,Norway rat,mouse and dog.

应用mRNA差异显示技术，对比研究正常与白化大菱鲆有眼侧皮肤组织，克隆差异表达基因，经测序，RT-PCR 验证，差异表达基因片段DEN-1(GenBank登录号：DQ886390)与DEN-2(GenBank登录号：DQ886391)均在白化大菱鲆有眼侧皮肤组织中下调表达；通过BLAST检索发现，DEN-1与GenBank中的斑马鱼和牛的类似尿苷二磷酸-葡萄糖：糖蛋白葡糖基转移酶基因、与挪威鼠类似尿苷二磷酸-葡萄糖：神经酰氨葡糖基转移酶1(Ugcgl1)基因有较高同源性；DEN-2 与原鸡、斑马鱼、人、挪威鼠、家鼠和狗的眼缺乏同源框4(eya4)基因的同源性均较高。

The acute toxicity test was conducted for lindane upon zebrafish, and histopathological effects of lindane on gill and liver of fish exposed to three concentrations of lindane 0.01μgL^(-1, 1.0μgL^(-1), 100.0μgL^(-1) for 36 days were determined by light microcopy.

以斑马鱼为受试生物，初步探讨了林丹对斑马鱼的急性毒性以及不同浓度0.01μgL^(-1、1.0μgL^(-1)、100.0μgL^(-1)的林丹暴露36d后对斑马鱼鳃和肝组织结构变化的影响。

Part IIMorpholino-mediated translational inhibition and overexpression oftbx2 in zebrafish cardiogenesis Objective To elucidate the role of tbx2 in zebrafish cardiogenesis via morpholino-mediated translational inhibition and overexpression on the base of expression pattern of tbx2. Methods Gene-specific antisense morpholino oligonucleotides were designed to against ATG region of zebrafish tbx2 to knockdown endogenous tbx2 gene.

设计tbx2-MO和对照MO，在斑马鱼受精卵的单细胞期，进行不同浓度的显微注射，并通过荧光蛋白融合标记实验和tbx2-mRNA、tbx2-MO共注射拯救实验，验证tbx2-MO抑制tbx2基因表达的有效性和特异性；基因过表达是通过给斑马鱼受精卵注射体外合成的tbx2-mRNA，来研究tbx2基因功能获得对斑马鱼胚胎心脏发育的影响。

Based on the study on cytogenetics of zebrafish, the genomes were compared between zebrafish and human, and the zebrafish Hox gene clusters were mapped to its chromosomes.

本文在斑马鱼的细胞遗传学研究的基础上，对斑马鱼与人类基因组进行了比较研究，同时对斑马鱼Hox基因簇进行了染色体定位研究。

The mutagenicity of seed powder and seed oil of transgenic CryIA cotton to mammals and fish was evaluated in this study. The raw cotton seed powder or seed oil was mixed into mice and fish's diet. Cyclophosphamide and NaF were used as positive controls respectively. Regular cotton seed powder and seed oil were used as negative controls. The micronucleus frequency in mice bone marrow polychromatic erythrocytes and in eripheral erythrocytes of Zebrafish were assayed in this study. The sperm malformation rate in mice was also investigated.

摘 要：分别以环磷酰胺和氟化钠作为阳性诱变剂，以普通棉的棉籽粉及棉籽油作为对照物，同时设阴性对照组，以成年封闭群昆明种雄性小鼠和斑马鱼为实验材料，将棉籽粉或棉籽油掺入饲料中饲喂小鼠或斑马鱼，检测小鼠骨髓嗜多染红细胞（Polychromatic erythrocytes， PCE）微核率（Micronucleus frequency， MN‰）和精子畸变率，以及斑马鱼外周血红细胞微核率。

The mutagenicity of seed powder and seed oil of transgenic CryIA cotton to mammals and fish was evaluated in this study. The raw cotton seed powder or seed oil was mixed into mice and fish's diet. Cyclophosphamide and NaF were used as positive controls respectively. Regular cotton seed powder and seed oil were used as negative controls. The micronucleus frequency in mice bone marrow polychromatic erythrocytes and in eripheral erythrocytes of Zebrafishwere assayed in this study. The sperm malformation rate in mice was also investigated.

分别以环磷酰胺和氟化钠作为阳性诱变剂，以普通棉的棉籽粉及棉籽油作为对照物，同时设阴性对照组，以成年封闭群昆明种雄性小鼠和斑马鱼为实验材料，将棉籽粉或棉籽油掺入饲料中饲喂小鼠或斑马鱼，检测小鼠骨髓嗜多染红细胞（Polychromatic erythrocytes， PCE）微核率（Micronucleus frequency，MN‰）和精子畸变率，以及斑马鱼外周血红细胞微核率。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。