斑点

The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

The experiments showed that arcing of sub-spots depends on emission of the first spot.

实验表明，次级斑点产生机制与首发射存在较强的依赖关系。

RESULTS In the thin-layer chromatogram of Rhizoma Arundo donax L., the spots of the main alkaloids were round, clear, and well separated.

结果 芦竹根药材薄层色谱图中，各主要生物碱类成分斑点圆整、清晰、分离度好。

Low temperature dispersivity of ashless dispersant TEPA was analyzed by the sludge spot distribution test.

以油泥斑点分散试验法对合成产物聚异丁烯丁二酰亚胺无灰分散剂进行低温分散性能测试。

Totally 31 patients with bacteriologically or histologically confirmed extrapulmonary tuberculosis in Peking Union Medical College Hospital received T-SPOT.TB assay to detect early secreting antigen target 6 or culture filtrate protein 10 peptides-specific T cells in the peripheral blood mononuclear cells.

对北京协和医院根据细菌学或／和组织学诊断的肺外结核患者31例，应用酶联免疫斑点技术检测6kD早期分泌靶向抗原和10kD培养滤过蛋白肽段库刺激后外周血中释放γ-干扰素的特异性T细胞。

Evergreen shrub or small tree whose foliage is conspicuously blotched with red and yellow and having small black fruits.

常青灌木或小树，它的叶子带着明显的红色和黄色的斑点，有小黑果实。

Any of various pale blotched snakes with a blunt snout of southwestern North America.

北美洲西南部的各种钝鼻并带有灰白色斑点的蛇。

It was the two children who finally routed the nurse by reminding her of a desiccated carcass, blotched with brown, streaked with yellow, scarred by knives.

正是这两个孩子迫使护士联想起那个带着棕褐的斑点、灰黄的采纹和刀伤疤痕的干枯躯体。

They spend too much time indoors and too much in the direct sun, and their skin has a pallid, blotched, flabby, exhausted look to it.

他们要么时时呆在屋里，要么暴晒太阳，以至于他们的皮肤毫无血色，斑点累累，松弛而又疲惫不堪。

Flowers pale to mauve-purple, blotched darker, perianth tube 5.5--6 cm, inner segments erect

花对淡紫色紫色，斑点的深色的，花被筒5.5浅--6厘米，内部裂片直立 57 IRIS kemaonensis 库门鸢尾

The reasons of iron ions content overproof in grade Ⅱ desalting water system,such as variation water quality,contamination of regenerant , operation adjustment of pretreatment system and switching operation of bed were discussed.

对二级脱盐水系统中铁离子含量超标的原因，如来水水质发生波动、再生剂受到污染、预处理系统操作调整、床体运行切换等进行了论述。

You were hired to drum up new business, so go and do it.

公司雇你招徕新业务，你就做你的事好了。