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Methods The circulating testosterone level of BPH patients and rats was decreased by administration of medroxyprogesterone and stilbestrol as well as castration. Using RNA dot blot hybridization technic, we detected the Bcl-2mRNA expression in human BPH tissue and rat prostate under different level of androgen.

分别通过服用药物和切除睾丸改变BPH患者和大鼠机体雄激素水平,采用mRNA斑点杂交技术,检测不同雄激素水平下的人BPH组织和大鼠前列腺组织Bcl-2 mRNA的表达情况。

Only a few conditions are known to cause this feature, which is called basophilic stippling.

据了解,只有少数情况会导致这种特征的斑点,被称为嗜碱性点彩。

Model was stitched over two threads on 32-count Stoney Point linen by Wichelt Imports.

效果图是两股缝在32ct大理石斑点进口Wichelt亚麻布上的。

She had short stumpy legs and long floppy ears, with a fawn-colored coat and tiny freckles sprinkled across her muzzle

她有粗短的腿,松软下垂的长耳朵,长着浅黄褐色的毛,嘴上有小斑点

Polyclonal antibody of Sulphamethoxazole,Sulfanilamide,Sulfadimethoxine were prepared, The establishment of dot-immunogold filtration assay for rapid dection of sulfamethoxazole residues would be a base for the use of DIGFA in dectecting residues of sulfanomides.

本研究制备了三种磺胺药的多克隆抗体,建立了快速检测磺胺甲噁唑的斑点免疫金渗滤法,为该技术在畜产品中磺胺药残留检测上的应用奠定了基础。

Results (1) The forward and reverse subtracted cDNA libraries of different metastastic potential large cell lung cancer cell lines were successfully constructed;(2) With blue-white colony and dot blot, 307 positive clones in the forward subtracted library and 78 positive clones in the reverse subtracted library were obtained;(3) 55 clones were successfully sequenced in the forward subtracted library. Homolog analysis confirmed 23 differential expression segments, which were similar to the human genes already known, including NME2, NPM1, MT 2A, HSPE1, TAFIA, EPRS, PX19 and EIF3S9 et al;(4) 31 clones were successfully sequenced in the reverse subtracted library. Homolog analysis confirmed 16 differentially expressed segments. 15 of them were similar to the human genes already known, including ANXA2, TUBB, PKN2, GNAS, EEF1A1, SSR2 and RPLPO et al. Only one segment had partial homology to known human genes. This segment was supposed to be the new EST segments which might not have been cloned.

结果(1)成功构建人大细胞肺癌高低转移株差异表达基因正向消减cDNA文库和反向消减cDNA文库;(2)经蓝白菌落筛选和斑点杂交,正向消减文库获得307个阳性克隆,反向消减文库获得78个阳性克隆;(3)正向消减文库挑选55个克隆成功测序,经同源性分析最终确定差异表达基因片段23个与已知人类全长基因具有很高相似性(95%~100%),这些基因包括NME2、NPM1、MT2A、HSPE1、TAFlA、EPRS、PX19和EIF3S9等;(4)反向消减文库挑选31个克隆成功测序,经同源性检索和比对最终确定差异表达基因片段16个,其中15个与人类全长基因具有很高相似性(95%~100%),包括ANXA2、TUBB、PKN2、GNAS、EEF1A1、SSR2和RPLPO等基因。1个片段和己知人类基因仅有部分相似性,表明它可能是未被克隆的人类基因EST片段。

Some of the breeds you will see include Longhorn Cattle, White Park Cattle, Wensleydale Sheep, Tamworth Pigs and Gloucester Old Spot Pigs etc.

其中我们在这儿可以看到的一些品种包括长角牛,白角牛,温斯利代长毛羊,塔姆沃思猪(一种皮毛呈红棕色的英国种肉用猪)和格洛斯特斑点猪等等等等。

Large feline of African and Asian forests usually having a tawny coat with black spots.

非洲和亚洲森林中的大型猫科动物,皮毛通常是茶色带黑色的斑点

A cat with a gray or tawny coat mottled with black.

有黑色斑点的灰色或棕色猫。

In wheat, during mitotic division H3 phosphorylation started in special domains of the nuclei from early prophase and vanished at telophase, remaining mainly in the pericentromeric regions of chromosomes at metaphase and anaphase.

有意义的是分裂末期这列荧光斑点的衰退在时间上与动物细胞的胞质分裂和染色体的解凝集相偶联。

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I didn't watch TV last night, because it .

昨晚我没有看电视,因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来,在北京的很多小区里,电梯液晶电视被撤了下来。

I'm running my simile to an extreme.

我比喻得过头了。