整合

The protein expression of 3 integrin subunits also started to change from 24 h of rotation in clinostat.

整合素亚单位蛋白和基因表达的变化相似。

RESULTS: The gene expression of 3 integrin subunits started to change from 24 h of rotation in clinostat but not in stationary cultures.

结果： 从模拟失重24 h开始，整合素亚单位的基因表达即发生改变，但在对照组中，基因表达无明显变化。

AIM: To investigate the expression of integrin subunits in osteoblasts during simulated weightlessness induced by clinostat.

目的：观察回转器模拟失重对成骨细胞整合素亚单位表达的影响。

METHODS: Calvarial osteoblasts of neonate rats were cultured under conditions of normal gravity(1G) and simulated weightlessness induced by clinostat respectively. The total RNA in cells was isolated in different time points (24, 48, 72 h). Reverse transcription PCR analysis was made to examine the gene expression of α5,αv and β1 integrin subunits.

培养的大鼠乳鼠颅骨成骨随机分为对照组和失重组，在实验的24， 48和72 h提取细胞总RNA，进行反转录PCR，检测整合素的α5，αv和β1亚单位mRNA表达，并计算与内参照β肌动蛋白 beta？？？？

METHODS: Calvarial osteoblasts of neonate rats were cultured under conditions of normal gravity(1G) and simulated weightlessness induced by clinostat respectively. The total RNA in cells was isolated in different time points (24, 48, 72 h). Reverse transcription PCR analysis was made to examine the gene expression of α5,αv and β1 integrin subunits. Each value was normalized against that of β actin mRNA.

培养的大鼠乳鼠颅骨成骨随机分为对照组和失重组，在实验的24， 48和72 h提取细胞总RNA，进行反转录PCR，检测整合素的α5，αv和β1亚单位mRNA表达，并计算与内参照β肌动蛋白 mRNA水平的比值。

Advertising Awards, as well being the only Chinese agency last year to win at Cannes, Clio and

且在整个2006年更加聚焦于提高策划和整合行销服务能力。该公司依旧在包括中国广告节在内的一系

Development in the industry and supply chain forming with: the worth system constituted by supplier, enterprise, closed path constructs the core competencies of supply chain integration.

在产业发展及供应链形成以：供应商、企业、通路所组成的价值系统，建构供应链整合的核心竞争。

Wi-Fi speed, high reliability, in an open area, communication distance up to 305 meters, in the closed region, communication distance of 76 meters to 122 meters, to facilitate comparison with the existing wired Ethernet network integration, network the advantages of low cost.

Wi-Fi具有速度快，可靠性高，在开放性区域，通讯距离可达305米，在封闭性区域，通讯距离为76米到122米，方便与现有的有线以太网络整合，组网的成本低的优势。

In the field of art collecting, and it always takes form of closed-door discussion even the

艺术推介：本届论坛的两个展览都是以东西方文化之间的整合与碰撞为主题，把中国

Among them,the companies of chemical fiber,clothing,the wool spinning,and the integrated operations companies possess relatively large rate,which are 19.4％,14％,and 13.1％ respectively.

在他们、组化学的纤维、衣服，羊毛纺织和整合的操作公司之中持有相对大的比率，分别是 19.4％，14％，和 13.1％。

What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢？

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我，一个心中布满虚荣的女孩子真实的想法。