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MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育,MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用;RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1),以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况;Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达,以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用;免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达;激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位,并观察真菌刺激后人角膜上皮细胞骨架的变化;流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变;光学显微镜观察真菌与人角膜上皮细胞黏附数量,记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后,细胞超微结构的改变。

Exchanging security information between reliantly parties, it defines a mechanism for exchanging authentication and authorization information among different systems, Single Sign On is an effective method which implement Integrated authentication and authorization.Base on analyze traditional SSO ,this paper discussed an effective application system using SAML and PKI/PMI technology , which resolved Security information exchange among heterogeneous system through once authentication realize full access. This technology was used successful in the project of "third of CTAIS".

构,它定义了不同系统之间安全服务交换认证和授权信息的机制,单点登录技术SSO是实现集成身份认证和授权服务的有效方法,通过对传统的SSO分析,提出一种基于SAML、利用PKI/PMI的单点登录企业级的应用系统,有效解决跨域系统和异构应用平台的一次认证、全程访问的安全信息交换;成功在&金税三期&资源整合中界面整合及门户项目得到应用。

These patterns could result from REMI events, tandem integrations, or a combination of both.

这些现象可能是质粒多位点串联整合并且整合后发生重组的结果。

SSL/TLS renegotiations enable servers to fine tune the security parameters or access controls for individual clients without having to reconnect.

它减少了将SSL整合进嵌入式工程的复杂性,使用一个简单的API和安全层,用户可以很容易地将MatrixSSL整合到它们的应用程序。

It uses the term "RUrban" to highlight the sustainable integration of rural and urban communities that these changes require, with rural living standards coming to match those in urban areas, and cities becoming net producers of ecological services rather than relying on regional and global resources and waste sinks.

论文用&远郊&这个词来突出郊区和市区整合的可持续性,整合带来的变化需要郊区的生活水平达到市区的水平,并且市区不仅仅再依赖地区和全球资源供给和垃圾接受,而是变成社会生态链条中的服务提供者。

Compression and inversion action make series of traps, tectonic traps and stratigraphy traps are the primary trap types, Tectonic traps can be classified into anticlinal traps (compressional anticlinal trap, drape anticlinal trap, faulted anticlinal trap) and fault block trap (scalariform fault block trap, arcuate fault block trap, crossed fault block trap). Stratigraphy traps can be classified into stratigraphic onlap trap and stratigraphic erosion unconformity trap.

后期受挤压、反转作用的影响形成了一系列的圈闭,主要的圈闭类型有构造圈闭和地层圈闭,构造圈闭又可划分为背斜圈闭(挤压背斜圈闭、披覆背斜圈闭、断裂背斜圈闭)和断块圈闭(阶梯状断块圈闭、弧形断块圈闭、交叉断块圈闭);地层圈闭可分为地层超覆不整合圈闭和地层削蚀不整合圈闭。

After an interval of four centuries in which men were absorbed by the problems of the political intergration of Europe, the interest in the problem in language was revived with the rise of Schola.

在四个世纪的整合后,在欧洲,人们吸收了各种政治上整合的问题,因为Schola的盛行,引起了人们对语言的兴趣。

According to previously announced programme, Japan Dentsu will integrate China Power Group's three companies in the Internet advertising, online media and business services, namely the Beijing Dentsu Advertising Co., Ltd., Beijing Orient Advertising Co., Ltd., the Shanghai East camp Advertising Co., Ltd. At the same time, Focus Media will also include integration (Allyes - All Yes, Kesi WorldCom, the miracle of creation, Ka Wah's constant, constant - Guangzhou, Shanghai stockwork), its six ad Companies, and interactive network field survey company resources, both owned by sharing technology, databases, business, and so on the strengths and improve the efficiency of use of media resources, provide better customer System Solutions.

据此前公布的计划,日本电通将整合中国电通集团旗下三家公司的互联网广告、网络媒体业务和服务,它们分别是北京电通广告有限公司、北京东方日海广告有限公司、上海东派广告有限公司;同时,分众传媒也将整合包括(好耶-All Yes、科思世通,创世奇迹,嘉华恒讯,广州恒讯,上海网脉)在内的旗下6家广告公司,以及互动网络领域调查公司资源,通过共享双方拥有的技术、数据库、业务能力等方面的优势,提高媒体资源利用效率,为客户提供更好的系统解决方案。

The enterprise has to be continually challenged by globalization through integrated information, strategic thinking, future planning, and new business plans, to integrate all the above resources internally and externally.In addition, the enterprise needs to refresh workable resources, technology, product and strategy and continually make changes with re-modeling, re-engineering, and creativity to promote the competitive strength and operation excellence so as to synergize information flow, material flow, cash flow and business process flow.

企业要经得起全球化的挑战,必须以各类取得的资讯做客观的分析、策略性的思考,来规划及发展出具前瞻性、适切性的企业经营新模式,而其重点即为将企业内、外部资源作有效的整合,在技术、产品与策略上推陈出新,因时因地的调整经营策略,进而转型、再造、创新,以提升企业之竞争力及傲人的营运绩效,以促成傲人的资讯流、物流、金流与商流的整合

This paper introduces synthetically development of theory about Integration after M&A at home and abroad.

本文针对企业并购后的整合理论在国内外的发展情况进行了梳理,为企业并购后整合的深入研究奠定基础。51-论文-网-欢迎您

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推荐网络例句

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车,和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特,那个是爱朗。

Articulate the aims, objectives and key aspects of a strategic business plan.

明确的宗旨,目标和重点战略业务计划。