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It has been reported that F185K and C280S mutations of HIV1 integrase would improve the enzyme solubility, and the catalytical activity of the enzyme was the same as that of the wildtype enzyme in vitro.

coli中进行整合酶基因表达,SDSPAGE鉴定表达产物,亲和层析纯化蛋白,酶联免疫吸附实验方法测定整合酶的生物学活性。

With research results from foreign scholars', a questionnaire of entrepreneurial organizational learning assessment has been established. Based on the 193 qualified samples from more than 53 entrepreneurial enterprises, confirmative factor analysis was conducted.

第一个子研究结合了March(1991)组织学习理论中探索式学习和开发式学习概念界定和分类方法,以及认知学习和行为学习的理论基础,提出一个整合的组织学习内容构思框架,将研究一得到的五种组织学习要素整合到这个构思框架中。

This conclusion is based on the common description of the contacts between and within "Tiaojishan" and "Tuchengzi" units as being disconformable and concordant.

这一结论是基于以往的髫髻山组和土城子组之间为假整合或平行不整合的观点所得出的。

NET Web Service, dissertate the feasibility of integration through Web Service.Then the best project is made up.

NET平台整合分布式数据库信息系统的可行性,并结合公司需求做出最优整合方案。

It is safe to believe the falune of cultural integrity contribute to those mergered enterprises' low efficience and effectiveness.

忽视企业文化整合、文化整合失败是企业兼并低绩效的一个重要原因。

1,After transfected the mutated mtDNA of colorectal carcinoma,the mtDNA D-loop region of the transfected cells displays new mutation points.2,The external source pieces of the mutated mtDNA can integrate to nuclear genome after transfection.3,There's no differences in apoptosis between combinations after transfected the mutation of mtDNA in NIH3T3 and LST cells.4,The mutated mtDNA may affect the action mechanism of occurrence and development in colorectal carcinoma through affecting its mtDNA mutation or integrating exogenetic mtDNA to its nuclear which may cause the abnormal expression of oncogene or anti-oncogene.

(1)转染突变的大肠癌细胞mtDNA后转染细胞的mtDNA均可发生多处的突变位点。(2)通过转染后突变的外源性的mtDNA可以整合到核基因组内。(3)突变的mtDNA转染LST细胞及NIH3T3细胞后,不影响转染细胞的凋亡改变。(4)mtDNA的突变可能通过影响体细胞mtDNA的突变和通过外源性mtDNA在核内的整合从而影响癌基因或抑癌基因的表达异常,从而参与肿瘤的发生发展。

1,After transfected the mutated mtDNA of colorectal carcinoma,the mtDNA D-loop region of the transfected cells displays new mutation points.2,The external source pieces of the mutated mtDNA can integrate to nuclear genome after transfection.3,There's no differences in apoptosis between combinations after transfected the mutation of mtDNA in NIH3T3 and LST cells.4,The mutated mtDNA may affect the action mechanism of occurrence and development in colorectal carcinoma through affecting its mtDNA mutation or integrating exogenetic mtDNA to its nuclear which may cause the abnormal expression of oncogene or anti-oncogene.

(1)转染突变的大肠癌细胞mtDNA后转染细胞的mtDNA均可发生多处的突变位点。(2)通过转染后突变的外源性的mtDNA可以整合到核基因组内。(3)突变的mtDNA转染LST细胞及NIH3T3细胞后,不影响转染细胞的凋亡改变。(4)mtDNA的突变可能通过影响体细胞mtDNA的突变和通过外源性mtDNA在核内的整合从而影响癌基因或抑癌基因的表达异常,从而参与肿瘤的发生发展。线粒体DNA;D―环区;突变;质粒;pcDNA3.1;转染

Under the guidance of DPRIF integrating strategy, we set up an integreted SOFM-SVM model. Then we analyzed and optimized the model from the aspects of operating mechanism, data interface and function expandedness. The PCA method was introduced to reduce dimensionality and extract features, then to strengthen the clustering explanation; defined a CMI index, to ascertain the most effective or the best clustering number; A new Anti-NO algorithm was proposed to recognize and to filter the suspecious data in the sample; The medium result of SVM model was used to extract the borderline datas between two classified groups. This research compensated for the achievements of data recognition including pattern data, noises data and borderline data.

在DPRIF整合策略指导下构建了一个整合的SOFM-SVM模型,对该模型从运作机制、数据接口、功能扩展几个方面进行分析和改进:引入PCA方法进行数据降维和特征提取,用以加强聚类解释;结合统计聚类中的聚类误差概念定义一个聚类数有效性指标,利用SOFM算法中间结果进行指标求解,以筛选出有效或最佳聚类数;提出一种新的噪声识别算法用于对样本中的异常数据进行甄别和过滤;利用SVM模型的中间结果提取分类边界数据;进一步充实了包括模式类、噪声集和边界在内的模式识别成果。

In addition, we also found that fluorene increased integrin αvβ3 protein expression by transcription.

此外,芴会藉由促进整合素αvβ3 mRNA的表现,来增加整合素αvβ3蛋白表现。

In this thesis, we design and implement a Heterogeneous network Integration Support Node for the integration of heterogeneous wireless networks integration. Then, we propose a distributed HISN network architecture.

在这篇论文中,我们设计并实作了一异质网路整合支援节点用以整合异质网路,此外我们并提出了分散式的HISN网路架构。

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Shop assistant: Please check at the door.

店员:请您到门口柜台交费。

In the end, transportation of PAHs in the environmental matrix was preliminarily investigated and discussed according to PAHs data of soils and sediments from a certain region in Shanghai. The result indicated that there are two or more pathways on PAHs transportation in the environmental matrix.

最后,对上海特定地区内PAHs在环境介质中的迁移径途进行了分析讨论,结果表明上海市土壤和沉积物等环境介质中PAHs的迁移存在多种途径。

Quick stop! AIDS doctors while the side and say hello and colleagues mention a blood sample box, the Red Cross had sent blankets and so on, with patients有说有笑to go home.

快停车!防艾医生们一边招呼一边和和同事们提着血液采样箱,抱着红十字会送来的被子等,有说有笑地同患者往家走。