敲

Thereafter,DNA fragment of Hyl-1-Amp-Hyl-1 was subcloned into pBR322 vector,the resulting construct was then checked by PCR and restriction analysis for the proper configuration of the knockout vector pBR322-SA.

该载体与质粒pBR322分别用EcoRⅠ、PstⅠ双酶切后进行连接，得到基因敲除的重组载体pBR322SA，PCR 及限制性酶切分析，构建的敲除载体与设计结果相符。

I tapped the melon lightly, the way my mother does in the supermarket, listening for the hollow sound of the freshness: Pung, pung.

像母亲在市场挑瓜那样，我也在瓜上轻轻地敲了敲，听里面有没有空洞洞的瓜熟的声音。

I knocked at your door for a quarter of an hour.

我敲你的门敲了15分钟。

France knocked on the russet color door and a small whine answered,"Germany~, is that you?"

法叔敲了敲那棕褐色的门，里面传来一个细小的呜咽声，"路德~，是你么？"

He went to the tattered screen door and knocked. A small boy very close to his age opened the door.

他敲了敲那扇破破烂烂的门，一个和他年龄相仿的小男孩开了门。

So, I took a small hammer and dented the slide of the trombone enough so that it wouldn't slide (I guess I really wanted to play drums!).

所以，我拿了个小锤子敲我伸缩喇叭的伸缩端把它敲陷下去了这样就伸缩不起来了（我想我那时应该是想去打鼓吧～）。

When nothing seems to help,I go and look at a stonecutter hanmmering away at his rock.

当一切都无济于事的时候，我去看一个石匠敲石头，他一连敲了100下石头仍然纹丝不动。

When nothing seems to help,I go and look at a stonecutter hammering away at his rock perhaps a hundred times without as much as a crack showing in it.

当一切都看起来无济于事的时候，我去看一个石匠敲石头。他一连敲了100次，石头仍然纹丝不动。

Joseph played the drums, and sometimes the tambourine, in a band called Fields of Grass.

约瑟夫在一个名叫"草地"的乐队敲鼓，有的时候敲手鼓。

In the midst of all these convulsions of the bell mingled with the revolt, the clock of Saint-Paul struck eleven, gravely and without haste; for the tocsin is man; the hour is God.

在这一片狂敲猛打的钟声和喧腾哗乱的人声中，圣保罗教堂的时钟庄严舒缓地敲着十一点，警钟是人的声音，时钟是上帝的声音。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。