敏感

Chenopodium album,Solanum nigrum, and Amaranthus retroflexus were very susceptible to the herbicides. Polygonum persicaria and Abutilon theophrasti were relatively less susceptible to the herbicides, and Lycopersicon esculentum was not susceptible to it. The relationship between reduction rates of weed biomass and PPM values of weed leaves 2,4, and 6 days after treatment was established.

供试的6种杂草对该混剂的敏感性存在显著差异：红心藜Chenopodium album、龙葵Solanum nigrum和反枝苋Amaranthus retroflexus对该混剂最敏感，ED90值分别为47.65、71.67和29.17g/hm2；春蓼Polygonum persicaria和苘麻Abutilon theophrasti敏感，ED90值分别为96.91、114.20g/hm2；而番茄不敏感。

At 0-60 ℃, the maximum fluorescence intensity of GCS-EPF decreased on heating and the relationship between fluorescence intensity and the change of temperature was linear; At pH=0-13.5, its fluorescence intensity increased with basification and completely disappeared at very acidic pH.

结果表明，修饰后水溶性壳聚糖的水溶液、固体粉末和薄膜在520 nm附近具有较强的荧光发射，其荧光强度不仅在0-60 ℃时对度有较快敏感响应，同时在pH=0-13.5时对pH也有较快敏感响应，具有双重敏感响应，因此可将其作为温度荧光探针和pH荧光探针的高分子材料。

In view of practical problem during Sulige gas field development, this paper complete follow study content. Study material balance theory of two district compound gas reservoir. Carry out stress sensitivity experiment on matrix rock and labor created fracture core from Sulige gas field with America Core Company high pressure high temperature flow experimental instrument to study stress sensitivity change rule of different permeability matrix rock and created fracture core at the condition of time and again switch on and off well and different drawdown pressure. Carry out depletion experiment on matrix rock of different permeability serial combination with different kinds of allocation, using double core clamper serial connected experimental apparatus, to study gas well production performance of bi-zonal compound low permeability reservoir during low pressure production stage. At the basis of experimental study, establish single well simulation model to analyze development index infection caused by stress sensibility and production performance infection rule caused by inner formation and outer formation of bi-zonal compound gas reservoir permeability variation while considering stress sensitivity.

针对苏里格低渗气田开发中存在的实际问题，完成以下研究内容；研究了两区复合气藏物质平衡原理；利用美国岩芯公司高温高压流动实验仪，对取自苏里格气田基质岩芯和人工造缝岩芯进行应力敏感实验，研究不同渗透率基质岩芯和造缝岩芯在多次开关井和不同生产压差条件下的应力敏感变化规律；采用双岩芯夹持器串联实验装置，对不同渗透率串联组合的基质岩芯进行各种配产条件下的衰竭实验，研究两区复合的低渗储层气井在低压生产阶段的生产动态特征；在实验研究的基础上，建立单井模拟模型分析储层应力敏感性和非均质性对开发指标的影响，研究考虑应力敏感时两区复合气藏储层中内区和外区渗透率变化对开发动态的影响规律。

The experiment identified weed control spectrum of toxin and sensitivity to main crops. The result showed that dayflower and goosefoot were most sensitive to toxin with injury response of level 4, followed by redroot pigweed and eragrostioid with injury response of level 3 and level 2, respectively. Among the main crops, corn and sorghum were susceptible to toxin while wheat, soybean and peanut were insensitive.

粗毒素杀草谱和对作物安全性测试结果为：鸭跖草和藜对毒素最敏感，伤害反应为4级；反枝苋和虎尾草伤害反应分别为3级和1级；玉米和高粱对粗毒素敏感，小麦、大豆和花生对粗毒素不敏感。

The result showed that dayflower and goosefoot was most sensitive to toxin which injury response reached to level 4,then redroot pigweed and eragrostioid which injury response was level 3 and level 2 respectively.Among main crops corn and sorghum were susceptible to toxin while wheat,soybean and peanut were insensitive to toxin.

粗毒素杀草谱和对作物安全性测试结果为鸭跖草和藜对毒素最敏感，伤害反应为4级，反枝苋和虎尾草伤害反应分别为3级和1级；玉米和高粱对粗毒素敏感，小麦、大豆和花生不敏感。6。

Of the patients tested using CDC technology, 17 (3.8%) had a panel reactive antibody higher than 10% and 8 patients (1.83%) had a PRA higher than 25% for class I antibodies. Class I sensitized patients, defined as patients with a PRA higher than 10%, had significantly lower survival rates than class I nonsensitized patients ( P =.0035). Class I nonsensitized patients had survival rates of 79.4% at 1 year posttransplant, 63.2% at 3 years, and 45.4% at 5 years. Sensitized patients had 1, 3, and 5 year survival rates of 64.7%, 28.8%, and 9.6%, respectively.

使用CDC检测的病患之中，有17位(3.8%)群体反应抗体第一类抗体高於10%、有8位(1.83%) PRA超过25%，第一类抗体敏感病患定义为PRA高於10%者，相较於第一类抗体不敏感病患、存活率显著降低(P =。0035)；第一类抗体不敏感病患移植后一年之存活率为79.4%、3年存活率为63.2%、5年存活率为45.4%；第一类抗体敏感病患移植后一年、3年、5年之存活率分别为64.7%、28.8%、9.6%。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

To investigate the response of plant growth and antioxidant system to changes of root temperatures, roots of six cucurbit plants were exposed to three different root-zone temperatures: low (14℃), middle (24℃) and high (34℃), while aerial parts of plants were maintained at the same natural ambient temperatures (23-33℃). The six cucurbit plant species could be classified into three groups with figleaf gourd and turban squash being I group (heat-sensitive but cold-tolerant), cucumber and melon being II group (heat- and cold-sensitive) and bitter melon and wax gourd being III group (heat-tolerant but cold-sensitive), i.e.

为了探究植物生长与活性氧代谢对根际温度变化的响应，以六种温度敏感性不同的瓜类作物为试材(组Ⅰ，黑籽南瓜与美洲南瓜，耐冷但热敏感型；组Ⅱ，黄瓜与甜瓜，冷敏感与热敏感型；组Ⅲ，苦瓜与冬瓜，冷敏感但耐热型)，分别将其根系置于不同的温度环境中：低(14℃)、中(24℃)与高(34℃)三种温度条件下，而地上部保持与外界空气相同的温度(23-33℃)环境中。

Selecting identification index of low-potassium tolerance The correlation between the grain weight per ear stress index and sensitive character stress index is appraised in detail by the numbers based on the principle of highly actuating, simply manipulating, early identification and saving field.

根据穗部敏感性状和穗粒重之间胁迫指数的关系，可以分为不敏感和敏感两类，敏感类型有穗粒数、百粒重、穗轴重三种，各类型存在明显差别。

The stage which is most sensitive to high temperature is trachea colouring embryo(ji-3), while the stage sensitive to dry condition is body pigmentation embryo(ji-5), so these two development stages have been called high temperature sensitivity stage and dry condition sensitivity stage respectively. The gene of high temperature and dry condition sensitivity is defined as sex-linked temperature sensitivity gene, which exists in 21.5 location of Z chromosome linking closely to sch gene, and presents concealed heredity.

其中对高温最敏感的时期是气管显现期(己_3)，对干燥最敏感的时期是转青期(己_5)，这两个时期分别称为高温敏感期和干燥敏感期，高温干燥敏感性基因定义为伴性温敏基因，t与Z染色体上21.5位置的sch基因紧密连锁，呈隐性伴性遗传。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

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如果有任何问题，你可以随时联系我。