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PAP-C23, amplified from mature pokeweed antiviral protein gene by PCR, was cloned into the expression vector PET101, and then the recombinant plasmid was transformed into Escherichia coli BL21(DE3). The recombinant mutant PAP was expressed high-levelly induced with IPTG.

用KCL溶液染SDS-PAGE胶,切下表达的目的蛋白,冰浴研磨后用磷酸缓冲液溶解,再加入等体积的福氏不完全佐剂,乳化完全后,免疫家兔,制备抗PAP蛋白的多克隆抗体,间接ELISA法测定所制备的抗血清的效价为1:1000。

The coexpression pattern of these antigens provides a theoretic foundation for the development of a polyvalent bladder cancer vaccine.

同时,多种CTA的联合表达为多效价CTA疫苗在膀胱癌免疫治疗中的应用提供了理论基础。

And includes the following aspect: 1 The synthesis of dendritic polyvalent glycol-conjugates ligand were concluded which can provide a new method to find new medicine.

本文包括以下几个部分: 1 合成树枝状多效价糖苷配体是找到新的抗感染、抗病毒、抗HIV药物以及人体疫苗等的重要途径。

The list shall contain with regard to each herbal substance the therapeutic indication, the specified strength and the posology, the route of administration and any other information necessary for the safe use of the herbal substance.

该目录应包含每个草药物质的治疗适应症,指定的效价和剂量,给药途径和任何其他与草药物质的安全使用有关的必要资讯。

Immunomodulation therapy can shorten the time of mechanical ventilation and the use of pressor agent, and it does not increase the length of stay and the cost in ICU, and therefore the cost effectiveness is high. It also can prolong the long?term survival time.

免疫调理治疗可以缩短患者机械通气和使用升压药的时间,而不增加ICU的住院时间和费用,有良好的效价比,可以延长远期生存时间,充分显示免疫调理治疗是危重病抢救中成功的治疗策略之一。

The purgative biopotency determination can be used in quality control and evaluation of rhubarb.

致泻效价检测方法可用于大黄的品质评价和质量控制。

Result: There wasn't a good linear relationship between the purgative biopotency and content of anthraquinone.

结果:不同大黄样品的致泻效价总体趋势与总蒽醌( R 2=0.653 7)、结合蒽醌( R 2=0.553 6)的含量有关,但均未呈现良好的线性关系。

Different biological and ecological characteristics, virulence, sporulation, conidia bourgeon, Protea-Bse activity leve and colony growth of 6 strains of Beauveria bassiana were measured respectively. The results were calculated by grey relational analysis and the optimum sequence was built from the professional comparison of each strain's characteristics. Two strain's have a potential of appling in mass production, BD-B015 and BD-B038 were abtained. The revisory mortality to Laphygma exigua Hubner were 88.33% and 86.70%.The infected rate were 86.67% and 85.27%.

测定初筛获得的6个球孢白僵菌菌株的毒力、产孢量、萌发率、胞外蛋白酶活性及菌株生长速度等多个生物、生态学指标,从专业角度构建最优序列,经灰色分析和不同菌株毒力效价显著性比较,筛选出了两株具有较大生产潜力的优良菌株BD-B015和BD-B038,对甜菜夜蛾的校正死亡率分别为88.33%和86.70%,僵虫率分别为86.67%和85.27%。

Objective: To test if self-reference can enhance remembering negative emotional valence words more than semantic processing.

目的:检验自我参照加工是否比语义加工更能促进抑郁症患者对负性效价词的记忆。

Objective:To explore the relationship between the valence of antibody of pregnant woman in serum and Hemolytic Disease of Newborn.

目的 探讨夫妇血型、孕妇血型抗体筛选及抗体效价与新生儿溶血病之间的关系。

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But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊!不用提了。提到肉,真是糟透了。

Tristan, I have nowhere to send this letter and no reason to believe you wish to receive it.

Tristan ,我不知道把这信寄到哪里,也不知道你是否想收到它。