效价

A new method for the biological assay of vegetable purgatives based on the numbers of wet faeces excreted by groups of mice after dosing is described.

1本文详述植物性泻药的一种新的生物测定法，此法系用小白鼠在服药后所排出的湿粪数为基础，以定泻药的效价。

Pneumophila serogroup 5 antibody to develop two immuo-colloidal gold tests respectively. Main works listed below as:1、Preparation and identification of polysaccharide antigen of L. pneumophila Bacteria of LP1 ~ 7,9 and 10 were cultured on yeast agar buffer activated carbon for three to five days at 37℃, 5%CO2. Protein-free polysaccharide antigens were obtained after harvest in cells, extraction, deproteinization, dialysis and other steps. Their immunogenicities were verified by ultraviolet spectrophotometer full-wavelength scanning and Western blotting.2、Preparation and identification of rabbit anti-LP1 antibodies and rabbit anti-LP5 antibody Rabbit anti-LP1 and anti-LP5 antibodies were purified after rabbits were immuned with antigens isolated as described above. The purities of both antibodies were above 80% and the titer of blood serum 1:32 tested by double antibody sandwich assay.3、Development of colloidal gold immunochromatographic assay kit The size of colloidal gold particles in the kit was 25nm. The optimal concentrations for antibodies were 30μg/ml and the sensitized concentrations of NC membrane were 5 mg/ml.

主要研究工作从以下几个方面进行：1、LP1~7、9和10型多糖抗原的制备与鉴定将LP1~7、9和10型菌株分别接种在缓冲活性炭酵母琼脂培养基上，37℃、5%CO2的条件下培养，3~5天后洗下菌苔，经抽提、除蛋白、透析等步骤后得到基本无蛋白的LP多糖抗原，经紫外分光光度仪全波长扫描及Western blotting验证其抗原良好。2、兔抗LP1抗体和兔抗LP5抗体的制备与鉴定分别用LP1、LP5型多糖抗原免疫家兔，采用琼脂糖双向扩散试验检测，两种抗体血清效价均为1：32；饱和硫酸铵法提取抗体，SDS-PAGE检定其抗体纯度均达到80%以上。3、胶体金免疫层析检测试剂的初步研制采用柠檬酸三钠还原法制备约25nm大小的胶体金颗粒；分别制备兔抗LP1抗体、兔抗LP5抗体的金标探针，两种抗体的最适标记量均为30μg/ml；选择适当孔径的微孔滤膜为载体包被两种抗体，NC膜包被浓度均为5mg/ml。

After 6 times booster immunization, collected vein serum and then test titer.

每次免疫后7天，兔耳缘静脉取血，测抗体效价。

The system was also used for the detection of components ratio combining with titer test. The result was highly according to that of the HPLC.

另外，将该TLC体系与微生物效价检定法结合，可以较准确地得到各组分的含量比，通过与HPLC检测结果进行比较，发现结果基本一致。

Mothods: Cock embryo was used to isolate influenza viruses, and the virus titer was determined through hemagglutination test. The gene was amplified by RT-PCR technique from the isolated strain and sequenced, and the gene phylogenetic tree was analyzed.

采用鸡胚进行病毒分离，采用血凝试验初步确定病毒效价，用逆转录聚合酶链反应法扩增分离株的部分片段后进行克隆和测序，然后进行进化树分析。

We made all-round serological tests to the two RhD-- individuals,including identification of blood group、Coombs test、antibody screening and identification、antibody titer and HDN test,together with investigation of genealogical blood group to all family members by classic hemagglutination methods.

我们采用经典的血细胞凝集法，对2个体进行了血型血清学检测，包括血型鉴定、抗人球蛋白试验、抗体筛选与鉴定、抗体效价检测与新生儿溶血病检测，并对两个家系所有家系成员进行家系血型调查。

Currently, the anti-influenza antibody is measured by the hemagglutinin- inhibition test. Although it is simple, but the result is easily influenced by the quality and species source of the red blood cells used. Antibody titer determined by HI test cannot reflect the protection titer either.

血球凝集抑制试验(hemagglutinin- inhibition test，HI test)是一至今仍普遍使用来测定抗流行性感冒病毒抗体的方法，此方法虽然快速、简便，但测定结果容易因为红血球的品质及来源而受影响，且血球凝集抑制试验所测定出的抗体效价亦不能反应真正的保护能力。

METHODS The killing effects of NDV-L on cancer cells and normal cell were observed by means of plaque assays, HA titer test and MTT test. The inducing effects of NDV-L on apoptosis were observed by means of Giemsa's dying test, phase contrast light microscopy, transmission electron microscope, DNA extraction, and flow cytometry.

通过噬斑形成单位、HA效价、MTT法等方法检测NDV -L对细胞的杀伤作用；通过Giemsa's染色法、倒置相差显微镜、透射电镜、DNA凝胶电泳、流式细胞仪检测NDV -L诱导肿瘤细胞凋亡的作用。

The invention provides a human McAb to tetanus toxin, being a human antibody, which consists of an antibody variable region and an antibody constant region or not, having the ability of neutralizing the tetanus toxin, with the variable region gene and protein sequence indicated as the sequence table 1. The invention is characterized in that the antibody can not induce obvious allergic reaction, has higher titer and longer effect without any animal virus pollution, and can be produced in an unlimited quantity, which also provides the relating biological functions, the testing methods, the manufacturing method and the application.

A 本发明提供一种人源抗破伤风毒素单克隆抗体，其为人源抗体，包括抗体可变区，该抗体包括或不包括抗体的恒定区，该抗体具有中和破伤风毒素的能力，抗体可变区的基因和蛋白质序列如序列表1所示；其具有不会诱发明显的过敏反应，有较高的效价且长效，产品无动物病毒污染，可以无限量地生产；本发明还提供了其生物功能、测定方法、生产制造方法及其应用。

Indeed, it may be said, in general, that the peculiar excellence of the Scholastics as systematic thinkers consisted in their ability to take hold of the profoundest metaphysical distinctions, such as matter and form, potency and actuality, substance and accident, and apply them to every department of thought.

事实上，可以说，在一般情况，特殊卓越的scholastics作为系统构成的思想家，他们有能力把握最深刻的形而上的区别，如此事和形式，效价及现状，实质和意外，并将之适用到每一个部门的思路。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。