效价

Capture ELISA method was used to determine isotypes of the mouse monoclonal antibody and the indirect ELISA method was performed to determine the titers of McAbs.

捕获ELISA法测定小鼠免疫球蛋白亚型，间接ELISA法测定腹水效价；用两步硫酸铵法和G蛋白亲和层析法来纯化腹水。

The isotypes of the five anti-CT mAbs were identified as IgM heavy chain and λ light chain. For the purification of anti-CT mAb, Hitrap IgM Purification Column was used.

其中CT-3A与CT-9A单株抗体经大量生产以Hitrap IgM Purification Column纯化后，经间接型ELISA免疫分析抗体效价在1：15，625。

To prepare the phosphorylated PRAS40 (Ser183) antibody, We chosen 10-amino acid including Ser183 as antigen peptide through antigenicity and hydrophobicity analysis, hinged on keyhole limpet hemocyanin, and used the KLH-peptide to immunize rabbits. After antibody serum titer detection by enzyme linked immunosorbent assay, the antibody was purified with rProtein A sepharose fast flow and dephosphorylated antigen membrane. The antibody titrate reached 1:10 000 after purification and its special property was enhanced with absorption treatment of dephosphorylated antigen membrane. In addition, we used rabbit anti-PRAS40 antibody and the phosphorylated PRAS40 (Ser183) antibody to detect PRAS40 expression in several cell lines, including the normal cells HL7702, HEK293, tumor cells HepG2, A549 and S180. There were no quite difference among these cells; otherwise, we observed the decreased phosphorylation level of Ser183 after amino acid withdrawal treatment.

为了制备PRAS40(Ser183)磷酸化多克隆抗体，本实验通过蛋白疏水性抗原性分析设计多肽抗原，用其免疫家兔获得抗血清， ELISA检测其效价为1：10 000； Western blotting法检测发现，通过rProtein A Sepharose亲和层析纯化并经非磷酸化的抗原条吸附处理后的抗体可以明显提高磷酸化抗体的特异性；用PRAS40抗体及PRAS40(Ser183)磷酸化抗体对正常细胞HL7702、HEK293及肿瘤细胞HepG2、A549、S180的检测显示：磷酸化的Ser183在不同细胞中表达差异不显著，而在经细胞饥饿处理的HEK293细胞中却明显观察到了S183磷酸化水平随氨基酸含量降低而减弱的现象。

His arsenal of shots is terrifying for precision, potency and versatility, equally dangerous from short-range, long-range, dead-ball, first-touch or aerial situations, left-foot and right-foot indiscriminately.

他是阿森纳队的投篮可怕的精确度，效价和多功能性，同样危险的，由近程，远程，死球，第一次接触或空中的情况下，左脚和右脚不分青红皂白。

The microcolumn gel assay can measure the valences of serum IgG in pregnants rapidly and acc...

结论微柱凝胶技术能够快速准确的检测孕妇血清当中的IgG抗体效价，是产前预报母子血型不合而引起HDN的有效方法。

The titres of erythromycin in original strain and recombinant were 3.98 and 5.15g/L, respectively, i.e.

红霉素效价，原始菌株和重组菌株分别为3.98和5.15g／L，相当于重组菌株提高红霉素体积产率约29％。

The light chain of the McAb was indentified to be κ. Its titres of ascitic fluids were up to 1×10^(-6) by indirect ELISA.

制备单克隆抗体腹水，腹水的间接ELISA效价在1×10^(-6)以上。

If they choose to Thiazide diuretics or β-blockers as initial treatment of patients with complex, will be significant cost savings, resulting in better price efficiency.

若选择噻嗪类利尿剂或β—受体阻滞剂作为病人的初始治疗物，将节约大量成本，产生较好的效价比。

Objective To compare the effect of SSA antigens in human and calf spleens.

目的 比较人脾及牛脾中干燥综合征A型抗原的效价，为临床检验及研究提供价廉方便的组织原料。

Rabbit was immunized by the conjugated alginate-BSA (1.0mg/kg) for 40-days routine immunity method; ELISA method was used to examine the titration of anti-alginate serum; The first generation parotid acinar cells were chosen to divided five groups (group A :contrast, group B: BAS, group C: alginate, group D: anti-alginate serum, group E: alginate +anti-alginate serum), We examined the proliferation of each part by MTT method at four times (lh, 6h,12h and 24h); In the meanwhile, The growth and shape of parotid acinar cells were observed under phase contrast microscope and scanning electron microscope.16 of the immunized rabbits by alginate were divided equally into Al and Bl groups, 12 normal rabbits were divided equally into A2 and B2 groups as control groups, Each rabbit was affused by 0.3ml(40fil/ml)alginate though parotid duct once time two days.

将藻酸盐与BSA偶连形成具有较强免疫原性的Alginate-BSA交联物免疫兔，按抗原量1.0mg/kg接种于新西兰白兔皮内，间隔10d加强免疫1次，共加强4次后，利用间接ELISA法检测藻酸盐抗体效价：将原代培养的兔腮腺腺细胞按10~4个/ml接种到96孔板上，置入细胞培养箱继续培养3d，分为5组：A空白对照组、B牛血清白蛋白组、C藻酸盐组、D抗藻酸盐血清组和E抗藻酸盐血清+藻酸盐组，分别加入PBS、牛血清白蛋白、藻酸盐或新鲜抗藻酸盐血清继续培养，分别于1h、6h、12h和24h不同的时间点，取出96孔培养板，采用MTT法检测5组腮腺腺细胞增殖情况

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。