效价

Objective To study comparatively the different of micro?column gel,improvement and classic Coombs,test in the determination of anti?

目的：比较经典法、改良法和微柱凝胶法抗人球蛋白试验检测孕妇血清中IgG抗A效价的差异。

Objective To study comparatively the different of microcolumn gel,improvement and classic Coombs,test in the determination of antiAIgG titers of the pregnant women.

目的：比较经典法、改良法和微柱凝胶法抗人球蛋白试验检测孕妇血清中IgG抗A效价的差异。

The improvement and classic Coombs,test showed good agreement in their result ,but MCG has higher positive rate than classicCoombs,test ,which is necessary to decide reference value ofits results.

改良法和经典法检测孕妇血清中IgG抗A效价的结果具有良好的一致性，而微柱凝胶法阳性率明显高于经典法，需重新设定临床意义决定值。

Then, the hapten LBc and LBs was conjugated to bovine serum albumin with CDI method to form immuno-antigen; the haptens LBc, LBs and LBy was conjugated to ovalbumin with mixed anhydride method to form the the coating antigens.The new zealand rabbits were immunized by conjugate of LBc-BSA and LBs-BSA, and titres of anti-bifenthrin serum (5.12×10~4, 3.2×10~5) were determined by non-competitive indirect enzyme-linked assay procedure.

通过碳二亚胺法将LBc和LBs交联于牛血清蛋白作为免疫抗原，通过活泼酯法将分别LBc、LBs和LBy交联于卵清蛋白作为包被抗原，LBc-BSA和LBs为免疫原制备了联苯菊酯的兔抗血清，非竞争间接酶联免疫吸附分析方法测得其效价达5.12×10~4和3.2×10~5。

Pseudosciaena crocea were immunized with formalin-killed and heat-killed trivalent vaccines by immersion and injection, which was made up of four experimental groups.

采用0.5%福尔马林4℃灭活和65℃热灭活2种不同的方法制备三联疫苗作为抗原，以多次腹腔注射或浸泡的方式免疫大黄鱼，通过测定受免大黄鱼血清中抗体凝集效价变化并进行攻毒实验以评估保护效果。

Following that, the hybridoma cells were injected into the peritoneal cavity of BALB/c mouse.

间接ELISA法测定抗体的效价，Western-blot检测抗体的特异性。

Methods The titer and affinity of anti-A secreted by hybridoma were tested by tube method and slide method.

通过试管法和玻片法检测抗A抗体的效价和亲和力。

The titer degree of this hybridoma cell line remained unchanged after frozen in liquid nitrogen for three months.

将杂交瘤细胞株在液氮中冻存3个月后复苏，其分泌的单克隆抗体效价不变。

The potency of antigens were higher than 6.0 IU/mL. The antigen is suitable immunising horse for producing rabies immunoglobulin.Keywords : Vero cells;Horse serum;Antigen

第5，8天收获病毒滴度可稳定在7.0logLD50/ mL以上，灭活抗原具良好的抗原性，用NIH法测定效价达6.0IU/ mL以上，可用作抗原生产抗狂犬病血清。

Isotypes, titer and affinity of the Mabs belong to G1 subclass withκlight chain.

利用ELISA试剂盒对单抗效价、亚类、亲和常数进行分析，4株单抗都属于IgG1，轻链为κ链。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。