效价

Titers and affinity constant of McAb were determined with indirect ELISA, and cross reactivity of McAb with indirect competitive ELISA.

用间接ELISA检测该McAb的效价和亲和性，用间接竞争ELISA检测与其他结构类似物的交叉反应率。

The specific McAb against dimethoate is obtained in this experiment, but its titer and affinity constant are low so that it is not up to standard enough to prepare kit using the McAb.

通过杂交瘤技术，获得1株特异分泌抗乐果McAb的杂交瘤细胞，但McAb效价、亲和力都较低，与制备试剂盒的要求尚有较大的差距。

The McAb against dimethoate showed certain cross reactivity for omethoate and weak cross reactivity for other several analogous compounds, and it displayed low titers and a low affinity constant. The titers of McAb in cells supernatant and ascites were 1:32 and 1:1024, respectively. The affinity constant of McAb was 1.4×10^4 L/mol.

通过杂交瘤技术，获得1株特异分泌抗乐果McAb的杂交瘤细胞，其培养上清与腹水效价分别为1：32和l：1024， McAb亲和常数为1.4×10^4L/mol，与氧化乐果的交叉反应率为10.6%，与其他所测结构类似的农药交叉反应率均小于0.5%。

The immunological traits such as titer,affinity,sensitivity and specificity of this mAb were characterized.The results showed that the conjugation ratio of SM to BSA in SM artificial antigen was about 25∶1.The titer of supernatant hybridoma cell lines of 3F9-C4 was 1∶3.2×10~2 by indirect ELISA.The isotypes in ascites was IgG_(2a)/κ,the affinity constant was 8.4×10~(11)L/mol,IC50 was 8.99μg/L,cross-reactivity to dihydrostreptomycin was 109.6% and little cross-reactivity to other compounds.

结果表明，BSA-SM人工抗原分子结合比为1∶25；筛选出3F9-C4敏感特异的杂交瘤细胞1株，间接ELISA测定细胞培养上清，效价为1∶3.2×102，同种型为IgG2a/κ；腹水的亲和常数为8.4×1011L/mol；IC50为8.99μg/L，与双氢链霉素交叉反应为109.6%，与其他SM结构相似物和功能近似物无交叉反应性。

The immunological traits such as titer, affinity, sensitivity and specificity of this mAb were characterized. The results showed that the conjugation ratio of SM to BSA in SM artificial antigen was about 25:1. The titer of supernatant hybridoma cell lines of 3F9-C4 was 1:3.2×10^2 by indirect ELISA. The isotypes in ascites was IgG(subscript 2a)/k, the affinity constant was 8.4×10^11L/mol, IC50 was 8.99μg/L, cross-reactivity to dihydrostreptomycin was 109.6% and little cross-reactivity to other compounds.

结果表明，BSA-SM人工抗原分子结合比为1：25；筛选出3F9-C4敏感特异的杂交瘤细胞l株，间接ELISA 刚定细胞培养上清，效价为l：3.2×10^2，同种型为IgG(下标 2a)/k腹水的亲和常数为8.4×10^11L/mol；IC50为8.99μg/L，与双氢链霉素交叉反应为109.6%，与其他SM结构相似物和功能近似物无交叉反应性。

A competitive ELISA kit for detect ENR was developed with ENR mAb and its traits were tested.Two hybridoma lines were filtered and the best one was 4G1-B3,which secreted ENR mAb with indirect ELISA titers of 1∶1.024×10~6 in ascites.Isotype of the two mAb was IgG_1.ENR mAb had a high affinity constant with 9×10~(10)L/mol.The ENR-Kit had the linear detection range of 0.05～101.6μg/L,the sensitivity of 0.05 μg/L and a good sensitivity with an IC_(50) of 1.1μg/L to ENR,cross-reactivity to other compounds less than 0.01%.The average recoveres of ENR spiked in chicken,liver,fish and milk were 81.5%,87.6%,84.3% and 95% respectively.The coefficient variation was below 10%.

结果表明：筛选出的2株杂交瘤细胞，单抗亚类均为IgG1型，其中4G1-B3株的ENR mAb间接ELISA效价为1：1.024×106，亲和常数为9×1010L/mol；竞争ELISA试剂盒的线性检测范围为0.05~101.6μg/L、灵敏度0.05μg/L、半数抑制浓度(IC50)1.1μg/L，与其他化合物的交叉反应率均小于0.01%，鸡肉样、鸡肝样、鱼肉样和牛奶样的平均添加回收率分别为81.5%8、7.6%、84.3%和95%，不同基质添加恩诺沙星标准品做竞争ELISA对ENR-Kit检测结果影响小，试剂盒保存期6个月以上。

Factors involved in the expression of IFN -αwere studied. These included multiplicity of infection, cell inoculation concertration and cell growth phase. The results show that lower mulitiplicity of infection (M01.4PFU/cell) is beneficial to IFN-α production.The optimal cell inoculation concentration was 〓 cells/flask. The high efficient replication of BmNPV-IFN-α and expression were also due to the proper inoculation time of BmNPV-IFN-α, which was at 48-60 house after cell passage.

研究结果表明，重组病毒感染复数的改变对α-干扰素的产量的影响是时限性的，在一定低感染复数时（Mo1.4PFU），α-干扰素的产量较高；对于重组病毒的复制，最适的细胞接种密度为3.6～7.0×〓细胞/瓶；在细胞指数生长前期（48～60小时）接种重组病毒，对于获得较高的重组病毒效价和α-干扰素的产量都是有利的。

Under the optimum concentration of 1.5% glycerol in fermentation medium and feeding of 1% glycerol as precursor after 60 h of incubation, the titer of 2,668 μg/ml was obtained in shake flask culture, an increase of 7.4 times compared with the original strain.

以XC-5-48菌株为试验菌株，当培养基中甘油初始浓度为1.5%，并且在发酵60h时补加1.0%的甘油，摇瓶发酵的效价可达2668μg/ml，较出发菌株(318μg/ml)提高了7.4倍。

The ELISA was conducted to detect the antisera of mouse and presented good results.

间接ELISA检测结果显示，Tc-BSA、Tc-HSA两种连接物在四免后抗体效价均达到1：3200以上。

Results: The two rabbit antisera had a title of 1:20,000 and 1:200,000, respectively, and had good cross-reactions with manmade human PC-1 protein N terminal 46 amino acids.

结果：两个兔多克隆血清的效价分别为1：20，000和1：200，000，并能与人工合成的人PC-1蛋白N端的46个氨基酸发生良好的交叉反应。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。