效价

Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection. A signal significantly greater than background binding was observed from the third to the fifth round of selection on CHOEGFRGFP1 and CHOK1 cell, but a part of polyclonal scFv bound EGFR specially. About 45% of the selected clones contained a fullsized insert of 1 kb. One unique human antiEGFR scFv (F4scFv) was isolated by analyzing with cell ELISA and DNA sequencing.

结果 经过5轮筛选，细胞裂解液中洗脱出噬菌体效价有500倍以上增长；细胞ELISA结果显示多克隆pscFv与CHOEGFRGFP1细胞和CHOK1细胞均有明显结合，但有部分特异性结合EGFR；菌落PCR显示约45％克隆中含有完整的1kb scFv片断；经细胞ELISA、 DNA测序检测共获得1株EGFR特异性单链抗体，命名为F4scFv。

Cell ELISA was used to determine EGFR binding specificity of monoclonal pscFv on EGFR positive and negative cell. The number of individual EGFRbinding clones was determined with nucleotide sequencing. Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection.

以稳定转染的CHOEGFRGFP1细胞和未转染的CHOK1细胞分别作为EGFR阳性和阴性细胞，采用负筛选的方法进行筛选；通过比较每轮投入及洗脱出噬菌体的效价比以及细胞ELISA检测多克隆pscFv与阳性、阴性细胞结合情况对筛选过程进行监测；采用菌落PCR挑选含有全长scFv片断的菌落，进一步用细胞ELISA检测单克隆pscFv与EGFR阳性及阴性细胞结合特异性；挑选EGFR特异性单克隆pscFv采用DNA测序法确定克隆多样性。

Objective: To establish a turbidimetric method for the determination of tyrothricin buccal tablets of compound dequalinum chloride.

目的：建立比浊法测定复方地喹氯胺含片中短杆菌素的效价的方法。

To mensurate the titer of the specimen, this method compares size of the bacteriostatic rings to the vaccinal test organisms produced by the standard and the tested by using the diffusion effect of antibiotics in agar culture medium.

本法是利用抗生素在琼脂培养基内的扩散作用，比较标准品与供试品两者对接种的试验菌产生抑菌圈的大小，以测定供试品效价的一种方法。

We find that the changing laws of antibodies in egg yolk and serum of laying hen are almost consistent, and the antibody levei in egg yok are always (more than 15 days after first immunization) high than that in serum. When several bacterins are used simultaneously, they can work in cooperation with each other.

并对这些抗体的动态变化规律进行观测，发现鸡血清抗体IgG与卵黄抗体动态变化规律基本一致，且蛋黄抗体IgY效价在基础免疫15天后总是略高于同期鸡血清抗体IgG；另外发现多株菌在同时免疫时具有协同免疫作用。

We find that the changing laws of antibodies in egg yolk and serum of laying hen are almost consistent, and the antibody Ievei in egg yok are always (more than 15 days after first immunization) high than that in serum. When several bacterins are used simultaneously, they can work in cooperation with each other. The quadratic rotary combination design is used to study the isolation of water-soluble proteins from the Egg Yolk by simple dilution with water.

并对这些抗体的动态变化规律进行观测，发现鸡血清抗体IgG与卵黄抗体动态变化规律基本一致，且蛋黄抗体IgY效价在基础免疫15天后总是略高于同期鸡血清抗体IgG；另外发现多株菌在同时免疫时具有协同免疫作用。

The reacting tite with NDPK-A was 1:800 and theantisera was specific.Conclusions: The purified rhNDPK-A protein exists mainly in the form of hexamer;rhNDPK-A protein can promote the survival of spleen cell and may take part in theadjustment of cell growth by the way of changing cell cycle.

ELISA法检测所制备的抗体的反应效价为：1：800，western blotting试验结果证实，该抗血清具备较好的特异性，能与rhNDPK-A蛋白发生反应，结论：rhNDPK-A蛋白可能主要以六聚体形式存在，直接观察的蛋白结构与文献报道的X衍射结果相似；rhNDPK-A蛋白对正常小鼠的脾细胞的存活有促进作用，推测很可能以信号转导的方式调控细胞周期从而达到对细胞生长的调节作用。

The agglutinating titers in serum of immunized mice and turtle could be as high as 29 and 27, respectively, on 21-35 days and 15 days after inoculation.

小白鼠注射疫苗后21～35 d，对这四株菌的血清凝集抗体效价最高达29，免疫保护率达100%，且对四株菌均有保护作用。

It was experimentally found that the average titer of antiserums was more than 12800 by indirect ELISA.

以PA-OVA为包被抗原，采用间接ELISA检测抗血清，其效价达到

Eventually, the highly specific anti-melamine polyclonal antibodies were acquired from antiserums through the purification of affinity chromatography.

采用酶联免疫吸附法测定各血清的效价，最终以亲和层析法纯化血清获得高特异性的抗体。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。