效价

The results showed that the lower agglutination titers were observed at day 7 and up to higher levels over 〓 or 〓 at day 21 induced by the vaccine of recombinant pilin and the recombinant fused protein respectively. Above results suggested that the good immunogenicity of the two recombinant vaccines could match with both the D.

结果纤毛蛋白基因工程疫苗在免疫7天后，被免兔血清中出现抗E型节瘤拟杆菌凝集抗体，21天后被免兔的血清凝集效价达到〓，与节瘤拟杆菌菌体灭活疫苗，尤其与纤毛蛋白基因工程疫苗对照免疫试验相一致。

Nodosus pilin and the killed D. nodosus vaccines. It also means that the IL-2 in the fused Pilin-IL2 construct may also have a potential role in stimulating vaccine antigen .Key words: D. nodosus Recombinant Pilin gene Recombinant Ovine IL-2 gene Fused Pilin-IL2 gene

用Pilin-IL-2融合基因工程疫苗免疫健康家兔的初步免疫试验结果表明，2l天后产生较高的凝集效价，达到〓，甚至稍高于加佐剂的前三种疫苗，证明绵羊IL-2基因与Pilin基因融合后，可起到一定的免疫佐剂作用。

Differences in prechallenge virus–specific antibody titers, demographics, season of the year, body mass, socioeconomic status, psychological variables, or health practices did not explain the observed associations.

给予病毒特定抗体效价前、人口统计学因素、季节、身体质量指数、社会经济状态、心理变项、健康实务都存有差异，无法解释前述关联；感觉到有休息的天数与临床感冒无关。

Differences in prechallenge virus–specific antibody titers,demographics, season of the year, body mass, socioeconomic status,psychological variables, or health practices did not explain theobserved associations.

给予病毒特定抗体效价前、人口统计学因素、季节、身体质量指数、社会经济状态、心理变项、健康实务都存有差异，无法解释前述关联；感觉到有休息的天数与临床感冒无关。

Immunohistochemistry analysis with Abl demonstrated the high expression level of CMTM4 in human testis, which was consistent with the previous resuit of Northem blot.

用多肽免疫家兔制备的2种抗CMTM4抗体不仅具有较高的效价以及特异性，并且Ab1还可以特异识别小鼠Cmtm4，为CMTM4的功能研究提供了有力的支持。

Methods Four hundred and sixty four patients from July 2006 to June 2007 who had been serologically tested for MP-IgM by using particle diameter agglutination were enrolled. The positive rate of MP-IgM in patients in different months, with different age and different clinical manifestations were compared.

用颗粒凝集法检测2006年7月至2007年6月我院儿科住院病房464例呼吸道感染患儿的MP抗体效价，比较不同月份、不同年龄及不同临床表现的患儿的MP-IgM阳性率。

The purified protein induced as antigen was used to immunize the rabbit, and the antiserum of WMV CP was prepared whose titer measured by ELISA was 1:6400; western blot analysis indicated that the antiserum could serologically react with CP.

以诱导的蛋白为抗原免疫家兔，制备了WMV CP抗血清，ELISA法测其效价为1/6400，Western blot分析能与CP发生血清学反应。

Members of the two types of families were compared for both HSV serological characteristics and CPT performance, and familial correlation in HSV infection was examined in tetrachoric correlation for categorical serostatus and in intraclass correlation coefficient for continuous antibody titers.

统计分析方面，分别比较精神分裂症病患家族成员及对照组家族成员之单纯？疹病毒血清学特徵与持续注意力。家族内成员的共同感染情形，藉由合适的相关系数指标予以量化：类别性的感染状态之相关程度以tetrachoric correlation呈现，而连续性的抗体效价则以intraclass correlation coefficient呈现。

Plasma specific antibodies IgA, IgG, IgM to Cpn were measured by microimmunofluorescence test. A group of individuals with Cpn specific IgA (titler:32) suggested chronic Cpn infection were compared with a group of control subjects with IgA (titler:32). Blood specimens were drawn for determination of serum lipids and Cpn antibodies. And the serum lipids profile were compared in the two groups.

横断面研究方法；采用微量免疫荧光法检测血浆中肺炎衣原体特异性抗体，以肺炎衣原体IgA升高（滴度≥1：32）的125名健康体检者为研究组，以性别、年龄近似但IgA必抗体效价不升高（滴度：32）的64名同期体检者为对照组，比较2组血清总胆固醇、甘油三酯水平的差异。

The efficiency of selection was monitored by comparing the number of phage recovered from the acid elution and cell lysate in each round,and by testing EGFR binding specificity of polyclonal phagescFv on CHOEGFRGFP1 and CHOK1 cell with cell ELISA. Bacterial PCR was used to select clones containing a 1 kb insert. Cell ELISA was used to determine EGFR binding specificity of monoclonal pscFv on EGFR positive and negative cell. The number of individual EGFRbinding clones was determined with nucleotide sequencing. Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection.

以稳定转染的CHOEGFRGFP1细胞和未转染的CHOK1细胞分别作为EGFR阳性和阴性细胞，采用负筛选的方法进行筛选；通过比较每轮投入及洗脱出噬菌体的效价比以及细胞ELISA检测多克隆pscFv与阳性、阴性细胞结合情况对筛选过程进行监测；采用菌落PCR挑选含有全长scFv片断的菌落，进一步用细胞ELISA检测单克隆pscFv与EGFR阳性及阴性细胞结合特异性；挑选EGFR特异性单克隆pscFv采用DNA测序法确定克隆多样性。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。