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Only one cell line named OHe which could secrete McAb stably was obtained.The isotype of the McAb was IgGi,the average number of chromossme of the hybridoma cell line was 97 respectively.

经鉴定该单抗为IgG_1亚类,杂交瘤细胞的平均染色体数目为97条,杂交瘤细胞培养上清及小鼠腹水单克隆抗体的ELISA效价分别为1:1024和

After the supplement of L-Leu, extracellular isovaleric acid was accumulated in vitro and then reabsorbed for eutilization, the ultimate relative content of isovalerylspiramycin II, isovalerylspiramycin III was increased by 41% and 50% respectively, and the relative content of total isovalerylspiramycins increased by 41.9% with the biological titer of biotechspiramycin being equal to that of the control group.

添加Leu对初级代谢影响不大,异戊酸在胞外积累,随后被重新吸收利用,最终异戊酰基螺旋霉素II和异戊酰基螺旋霉素III组分分别提高了41%和50%。同时总异戊酰基螺旋霉素相对含量也比对照提高了41.9%,效价和对照基本一致。

The results showed that capsule enhanced the ability of lymphocyte multiplication and macrophagocyte phagocytosis,and also increased the number of plague forming cell and the titers of hemagglutination against sheep red cell at 250 and 750 mg/kg BW.

实验结果表明,250和750 mg/kg剂量的富硒胶囊可提高小鼠淋巴细胞增殖能力、单核巨噬细胞吞噬能力、脾脏抗体形成细胞数和血清中抗绵羊红细胞抗体效价

Methods right sciatic nerve compression model of 120 male icr mice was employed to observe the effects of aerobic exercise on weight of immunity organs,abdominal cavity the phagocytosis rate of macrophagus in abdominal cavity,antibody potency and ear swelling index.

雄性icr小鼠120只,采用右侧坐骨神经卡压模型,观察有氧运动对小鼠免疫器官重量、腹腔巨噬细胞吞噬率、抗体效价和耳肿指数的影响。

To improve the ferment output of agricultural antibiotics 120 through mutagenic breeding.

通过诱变育种提高农抗120生产菌株的发酵效价

Results The IgY solution obtained from water dilution method has specific binding with Naja atra and the titration increased coinciding with purity.

结果鸡卵黄经水溶法的粗提物与中华眼镜蛇毒即有较明显沉淀反应,其效价随着纯度的提高而增强。

The acute virus necrobiotic disease virusC AVND viruswas isolated from diseased scallop Chlamys farreri collected in the Taiping Bay of Qingdao. Using purified virus as antigens, the polyclonal antibody with high titer was prepared in rabbits. The polyclonal antibody as the first antibody, IgG-HRP as the second antibody, indirect ELISA technique was set up through selecting the best interactional conditions among the first antibody, the second antibody and antigen.

以患病栉孔扇贝组织中提纯的急性病毒性坏死症病毒作为免疫抗原,注射新西兰白兔制备出效价高达1:32000的多克隆抗体作为第一抗体,第二抗体为辣根过氧化物酶标记的羊抗兔IgG,通过交叉稀释法确定抗原、一抗及二抗的最优反应条件,建立酶联免疫吸附分析方法体系,病毒检测灵敏度达30ng。

The preparation of monoclonal antibody of anti epithelial carcinoma of ovary by the cytomix method of oophoroma correlated antigen (CA 925 )was studied The antibody used was of IgG1 type.

采用自制卵巢癌相关抗原CA925经细胞融合方法,制备抗卵巢上皮癌单克隆抗体OC4D9,该抗体呈IgG1型,抗体效价ELISA104,抗体特异性较好。

Methods: Eighty mice were randomly divided into high, middle, and low dosage groups which were orally given 1.5 g/kg, 0.75 g/kg, 0. 375 g/kg of ant powder respectively, twice a day for 10 days. A blank control group was set up which was given water instead. Body weight, thymus weight and spleen weight were measured. Thymus indexes and spleen index were calculated. Neutrophil phagocytose rate, Ea and Et rosette formation rate of T lymphocytes, and hemolysin titre were detected. QHS values were achieved with spleen cell mediated sheep red cell spectrophotometer quantitative assay.

根据黑蚂蚁的用药量将小鼠分成高、中、低剂量及空白对照组,黑蚂蚁粉给药量按小鼠体重1.5g/kg、0.75g/kg、0.375g/kg计算,连续10d灌胃给药后,测定各组小鼠的体重、胸腺和脾脏重量,计算胸脾指数;检测中性粒细胞的吞噬率、T淋巴细胞E花环形成率及小鼠的溶血素效价和脾细胞介导的羊红细胞分光光度计定量测定法。

A blank control group was set up which was given water instead. Body weight, thymus weight and spleen weight were measured. Thymus indexes and spleen index were calculated. Neutrophil phagocytose rate, Ea and Et rosette formation rate of T lymphocytes, and hemolysin titre were detected. QHS values were achieved with spleen cell mediated sheep red cell spectrophotometer quantitative assay.

根据黑蚂蚁的用药量将小鼠分成高、中、低剂量及空白对照组,黑蚂蚁粉给药量按小鼠体重1.5 g/kg、0.75 g/kg、0.375 g/kg计算,连续10 d灌胃给药后,测定各组小鼠的体重、胸腺和脾脏重量,计算胸脾指数;检测中性粒细胞的吞噬率、T淋巴细胞E花环形成率及小鼠的溶血素效价和脾细胞介导的羊红细胞分光光度计定量测定法。

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