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In order to further explore the relationship between this effect and the synthesis of RNA and DNA in the neurons, an autoradiography of 3~H-uridine and 3~H-thymidine was used.

本文应用3~H-尿嘧啶核苷和3~H-胸腺嘧啶核苷放射自显影术,进一步研究该作用与神经元合成RNA和DNA的关系。

Different drug|resistance related genes in sgc7901/vcr were screened with staining silver and autoradiography mrna differential display method.

同时应用银染和放射自显影的mrna差异展示方法筛选胃癌sgc7901细胞和耐药细胞亚系sgc7901/vcr之间mrna表达的差异。

Methods Rat models of ischemic reperfusion in vivo were established. With autoradiography, the uptake of 99Tc-HL91 was observed in myocardium in control group, ischemic reperfusion group and ischemic group.

建立大鼠心肌在体缺血再灌注模型,采用体外放射自显影检测正常对照组、缺血再灌注组及无再灌注组心肌对99Tc-HL91的摄取。

In order to further explore the relationship between this effect and the synthesis of RNA and DNA in the neurons, an autoradiography of 3H-uridine and 3H-thymidine was used.

本文应用~3H-尿嘧啶核苷和~3H-腺胸嘧啶核苷放射自显影术进一步探讨此作用与神经元合成RNA和DNA的关系。

In vitro cleavage reaction was performed at 37℃ for 1h under the presence of Mg++. The cleavage product was analyzed by polyacrilamide gel electrophoresis. After autoradiography, the cleavage rate was counted by image analysis.

体外切割作用在Mg++的存在下,37℃反应1小时,进行聚丙烯酰胺凝胶电泳,放射自显影后经图像分析计算出切割率。

The transcription localizations of these polymerases have been studied since early 1960s. The results of these early research works showed that the transcription of RNA polymerase I was localized in the nucleoli while that of RNA polymerase Ⅲ in the nucleoplasm.

自上个世纪六十年代初期,人们相继运用细胞化学染色、电镜放射自显影等进行研究的结果表明:RNA聚合酶Ⅰ的转录发生在核仁中,RNA聚合酶Ⅲ的转录发生在核质中。

Autoradiographic studies have demonstrated that adenosine A1 and A2 receptors are expressed in the spinal dorsal horn, both on the post-synaptic SG neurons and the central terminals of the primary afferent neurons.

放射自显影的结果表明A_1和A_2受体都分布在脊髓背角,包括突触后SG神经元和初级传入神经元的中枢突,而A_1受体集中分布于胶状质。

The result of the SDS-PAGE showed that the protein content of Cereuisine fermentum cell dramatically decreased in the present of 20μg/mL NCS. It was only 68% of the control.

将酵母细胞培养在含S~(35)标记的甲硫氨酸培养基培养后,经放射自显影及细胞中S~(35)含量检测发现NCS处理后,酵母细胞中蛋白质合成受到抑制。

All of them were labeled with [32P] orthophosphate (2.03×10^5 Bq/mL) for 2 h and then stimulated with CCK8 l0^(-7 mol/L and DMEM respectively for different time. Reactions were terminated by freezing neurons in liquid nitrogen. The 32P-labeled lysates isoelectrically focused on IPG Drystrip pH 4~7 linear gels and subsequently separated by second-dimensional SDS-PAGE.

培养乳小鼠神经元,随机分为CCK8刺激组和对照组,32P-NaH2PO4标记后分别给予CCK810^(-7 mol/L和等量无磷培养基,作用不同时间后液氮终止反应,经双向电泳、放射自显影,获得磷酸化蛋白质的电泳图谱。

Methods: Immunoautoradiography method was used to reveal SERT immunoreactive intensity and the results were compared with those of healthy controls.

应用免疫放射自显影的方法,显示SERT免疫反应强度在酗酒人脑标本脑干头侧中缝核群的变化,并与健康人脑标本比较。

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