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An improved CTAB method was used to extract winter buds and tender leaf of ten Species of Spiraea DNA and factors which affect the RAPD amplification were optimized and selected.

试验以10种绣线菊属植物为材料,采用改良CTAB法进行冬芽和嫩叶的总DNA提取并对影响RAPD扩增效果的因素进行优化。

Having used 10 kinds of Spiraea resources for the material,respectively SDS method,high-salt low pH method and the improved CTAB method for DNA of young leaves of the extraction,detection and comparison;the use of tested template for RAPD primers screening.

以10种绣线菊属植物资源为材料,分别用SDS法、高盐低pH法及改良的CTAB法对幼嫩叶片进行DNA提取并进检测比较,利用经过检测的模板对RAPD引物进行筛选。

The spores of Cheilolejeunea ventricosa X.L.He were cultured on MS media (without acid and vitamin, pH=6.2, with 1% agar and 1% sucrose). The sporeling development and oil body ontogeny were observed. After a 55-day-long axenic culture, young plants were obtained successfully. Sodium hypochlorite solution was used to sterilize the capsules. Our results revealed that the sterilizing treatment with 0.1% Sodium hypochlorite solution for a period of 5 seconds was optimal. Oil bodies in primary protonemata are nearly homogeneous, but distinctly compound in the leaf cells of the primary leafy shoots.

首次研究了膨叶唇鳞苔的孢子萌发、原丝体和油体发育及配子体发生的过程,结果表明:(1)使用经过改良的MS培养基(不加氨基酸和维生素,pH值为6.2,琼脂和蔗糖浓度均为1%)能在55 d左右的时间利用孢子培养获得植物体;(2)用0.1%的次氯酸钠溶液,消毒5 s是消毒孢子的最佳方案;(3)油体在孢子萌发初期为均一型,到幼叶发生后,成为可见的聚合型,但聚合的程度比野生材料要弱。

In this paper, we overviewed the relationship between male gametophytic selection and phenotype of its sporophyte, genetic differentiation and its application in plant genetic improvement, so it would be provided reference information for correlated research of male gametophytic selection.

本文主要概述雄配子体选择与孢子体表型的关系、遗传分化效应及其在植物遗传改良中的应用,以期为雄配子选择相关研究提供参考

Main results shown as follows:Three methods—SDS method, simple CTAB method and improved CTAB method were used to extract genomic DNA from sporophyte of Laminaria.

主要结果如下:对分别用SDS法、常规CTAB法、改良CTAB法三种方法提取的海带基因组DNA,进行紫外吸收检测、电泳检测和RAPD扩增检测。

Glass doors; closer; hinge; bathroom glass doors; to spring; bolt; Hinge

碧辉五金是一家专业生产玻璃门夹、地弹簧、闭门器、玻璃门锁,浴室玻璃夹配套,不锈钢合页,幕墙五金配套,幕墙玻璃接驳爪等建筑装饰五金系列的制造商,公司在"不断改良,不断创新"的道路上打造了自己的品牌,并以其"优胜的质量,诚信的服务"取决于市场,深受广大使用者的信赖与支持;赢得广大顾客的青睐。

Some suggestions on improving spring wheat quality with high quality subunits and their combinations in Ningxia province were proposed.

提出了利用优质亚基及其组合在宁夏春小麦品种品质改良上的看法。

REAL SLUM AND squatter upgrading changes urban poor groups' relationships with city author-ities and other city actors.

真实的贫民窟和擅自居住者改良,改变了城市贫民群体和城市权力者及其他城市执行者之间的关系。

James Brindley of Staffordshire started his self-made career in 1733 by working at mill wheels, at the age of seventeen, having been born poor in a village.

斯塔福郡的詹姆斯布林德雷,出身于一个贫苦的农村家庭;1733 年,他才 17 岁,就着手改良磨房的车轮,从而开始了他自我奋斗的生涯。

Newly-introduced Star Apple is an example. Star Apple, also called Milk Apple, tastes like persimmon. It came from Caribbean Sea originally, and became very common in South-East Asian countries. Because its persimmon-like flavor, which is agreeable to most of Taiwan people, added is that it is easy to plant, NPUST decides to introduce and improve Star Apple in Taiwan.

最近研发种植成功的星苹果就是一例,星苹果又名牛奶果,原产地在加勒比海,现在在东南亚国家相当容易看见,因为吃起来像柿子,适合台湾人的口味,加上栽种容易,才会开始引进台湾,并著手改良、研发。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。