收集

In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2～0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%～80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0～0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各种纯化方法，将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合，建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺：胰酶用pH4.5醋酸缓冲溶液提取后，粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5，洗脱缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5)含0.2～0.35mol／LNaCl分两步洗脱，收集激肽释放酶的洗脱峰；DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液：0.01mol／LTris-HCl缓冲液(pH7.5，洗脱液：0.5mol／LNaCl溶液，一次洗脱，收集胰蛋白酶洗脱峰；最后，亲和层析未吸附液用30％～80％硫酸铵分级盐析处理，沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5含1mol／L(NH_4)_2SO_4，洗脱缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5)含0～0.6mol／L(NH_4)_2SO_4，分两步洗脱，收集糜蛋白酶的洗脱峰。

Famous Westlake is located in the southwest of the City of Hangzhou,the Capital of Zhejiang Province,used to be the capital of the Souther Song Dynasty. It became wellknown for its beautiful scenery with the culture of Chinese history, Buddhis

个别论坛/博客软件屏避汉语词汇的违法行为必须纠正 5242 个字符大庆广播电视大学商江收集整理大庆广播电视大学商江收集整理大庆广播电视大学商江收集整理 2009 年 7 月 16 日

Primer 325 RAPD showed all COS-PA from SICU in 2003 were identical (SICU-A type), but the Burn-A type and SICU-A type were distinctly different. Most of the non-COS-PA from SICU also showed the trend of prevalence, which was defined as SICU-B type in RAPD typing, including the strain found in the bottom of the washstand.

SICU 2003年收集的COS-PA，经RAPD（引物325）分型为同一型别；在SICU收集的non-COS-PA也多数呈同一型别，自SICU水池下水口收集到的1株non-COS-PA也属于此型。

The major direction of the research within the first year will be outlined as following:( 1 )The background study, collection, analysis, and identification of the possible metabolites such as protease, hemolysin, hemagglutinin, cytotoxin, leukocidin, and lipopolysaccharide;( 2 )The collection of the pathogenic factor such as outer membrane protein of vibrio spp.;( 3 )The analysis of pathogenic factor through the application of SDS-PAGE technique; and ( 4 )The activity assay of outer membrane protein on substrate to characterize the possible function or study the reaction mechanism.

致病因子之收集：将利用生物化学之方法，以弧菌属细菌外膜蛋白质为目标，收集与溶血素相关之致病因子。3。致病因子之分析：将利用SDS-PAGE电泳，对所收集得到之致病因子进行分析，并估算其可能之分子量。4。致病因子之活性测试：将利用所纯化之外膜蛋白质进行对受质活性之测试，以确定其功能或可能之作用机制。

To examine HHV-8 DNA in PBMCs, bone marrow mononuclear cells, bone marrow biopsy samples and biopsy samples of lymph node and lymphomatous tissue from patients with lymphoma, leukemia and multiple myeloma, to investigate the possible ...

材料和方法 1。标本收集(1)献血员和正常对照：采集正常献血员标本110份，并从中分离PBMCs110份，抗凝血30份，血清30份。收集反应性增生淋巴结标本20份。非血液系统疾病患者BMMCs15份。正常肋骨骨髓活检标本15份。(2)淋巴瘤患者：收集44例淋巴瘤患者外周血36份，BMMCs12份，淋巴瘤病。。。

According to the result showed at 280nm and at 490nm,in the comparison of whether protein absorption top and sugar quantity top overlapped,glycoproteins would be detected preparatorily,and as a result,tubes in two distinct areas had glycoproteins by this method. Proteins were precipitated with trichloroaceticacid and with cold acetone,and glycoprotein was determined from SDS-gel.

再从各收集管的收集液中，用三氯乙酸沉淀蛋白法、冷丙酮沉淀蛋白法相结合浓缩、制备蛋白样品，进行SDS—PAGE，对SDS胶进行PAS糖链染色鉴定糖蛋白，并从茶树叶分级蛋白中准确地鉴定出两个区域的收集管中含有多种糖蛋白。

Gravure press is not discharge on down during the mode of production, the per-color business card printing and membership card after harvest cylindrical insertreleased, offline after the subject with a single eyefor a cross-cutting machine rolled business card printing and membership card making for easier and more precise printing long broken sected collection, and then set the broken sected collected leaflets business card printing and membership card making products in spinons convex, indentation, die-cutting, waste, collection, testing, packaging; business card printing and membership card making products for stamping or compact label positioning easy-care, and the increase in the operation, the disadvantage is that the above process, the operation covers an area of large plant, to make more, and sometimes the drum to the materials offline operation in cross-cutting precision |interrupted Zhang superstandard, it will be a large number of categorizing people and a large area of the workspace.

凹印机是采用上料下料不停机的生产方式，进行逐色制卡和会员卡制作后收成卷筒状取下，离线后通过一台识标横切机把卷状制卡和会员卡制作品以单幅印长断切收集，再将断切收集来的单张制卡和会员卡制作品进行棘凸、压痕、模切、剔废、收集、检验、包装；制卡和会员卡制作品如需要烫金或镭射标定位烫，还需增加工序，以上工艺缺点是工序多，占地面积大，使用机台多、人员多，有时卷筒印材离线作业在横切过程中断张精度超标，还需大量的分检人员和大面积的工作空间。

This one integrated solution is compositive simple automation mechanism comes installation, configuration and processing network equipment, enea can offer top-ranking XML network to administer software for supplier of global network equipment from this.

这一整合解决方案集成了简单的主动化机制来安置、设置装备摆设和治理收集行动措施，Enea由此可为寰球收集行动措施供给商供给一流的XML收集治理软件。

Enea of advanced software firm and the leader Tail-f Systems that are used at XML network to administer announce global network equipment jointly today, both sides had signed contract of a strategy, the equipment network that leaves generation tall usability for telegraphic industry research and development together administers software.

寰球收集行动措施高级软件厂商Enea和用于XML收集治理的带领者Tail-f Systems明天连系公布，双方已经签定了一份策略合同，联手为电信行业研发下一代高可用性的行动措施收集治理软件。

This paper designs overall frame of Internet information collection and processing technology,proposes a search engine called IA,which is knowledge-based and adopts some techniques of data mining,filtrates and classifies collected information.

该文对互联网公开情报收集与处理技术的总体框架进行了设计，通过一个基于知识库的主动式专题搜索引擎完成专题情报的收集，并过滤与分类收集信息。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。