支气管肺泡的

Methods Models of severe thoracic trauma were established,alveolar macrophages were obtained by collecting broncho alveolar lavage fluid,and then separated and cultured.TLR4 mRNA and the expression of TLR4 protein were measured by northern and western blot before and 2,4,8,16,24h after trauma.

建立大鼠严重胸部创伤模型，支气管肺泡灌洗分离、培养肺泡巨噬细胞，利用Western、Northern分子生物学技术检测创伤前、创伤后2、4、8、16、24小时肺泡巨噬细胞TLR4的表达水平。

FPLT can be obtained after PLE was injected to the target lung and the target bronchia was obstructed with broncho occluder by catheter.

向靶肺支气管肺泡灌注PLE并用支气管封堵器堵塞靶支气管可达到功能性肺叶切除的目的，该方法安全、简便，术后对肺循环无明显不良影响。

Results: Amang 128 patients, 56 cases were diagnosed as pulmonary carcinoma (43.75%), of which 30 cases were squamous carcinoma (53.57%), 22 cases were adenocarcinoma (39.29%), 4 cases were small cell undifferentiated carcinoma (7.14%); 37 cases were bronchial pneumonia (28.91%), in which 30 cases were given brush sampling, bacterial culture of BALF, the detectable rate of pathogenic bacteria were 50%(15 cases) and 40%(12eases) respectively; 12 eases were pulmonary tuberculosis (9.38%), of which 3 eases (25%) had concurrent endobronchial tuberculosis, smear of brush biopsy and BALF found Mycobacterium tuberculosis were 11 eases (91.67%) and 5 eases (41.67%) respectively, 2 eases were diagnosed as caseous tubereulous granuloma (16.67%); 2 eases were idiopathic pulmonary fibrosis (1.56%), lease was lymphoma of long.

结果：128例患者确诊为肺癌56例(43.75%)，其中鳞癌30例(53.57%)，腺癌22例(39.29%)，小细胞癌4例(7.14%)；支气管肺部炎症病变37例(28.91%)，其中30例经防污毛刷采样、支气管肺泡灌洗液行细菌学培养，病原菌的检出率分别为50%（15例）和40%（12例）；肺结核12例(9.38%)，其中合并支气管内膜结核3例(25%)，刷检涂片和支气管肺泡灌洗液找到结核杆菌分别为11例(91.67%)和5例(41.67%)，病理确诊为结核性乾酪样肉芽肿2例(16.67%)；特发性肺间质纤维化2例(1.56%)，肺淋巴瘤1例(0.78%)。

We did bronchovesicular lavage with 10 mL normal saline, then collected all cells in BALF, stained cells by Giemsa's stain and counted the total number of cells and differential cells numbers for observing the inflammatory changes in lungs after RSV infection.

以10 ml生理盐水进行支气管肺泡灌洗，收集肺泡灌洗液中细胞，常规吉姆萨染色，行细胞分类计数，观察RSV感染对肺脏的炎症影响。

The results showed:(1) MP polymerase chain reaction test showed positive results in the bronchoalveolar lavage fluid from all of the MP-infected rats (infectious group, n=4), while they were all negative in BALF from the control group (n=4,P＜0.05) and from those rats both infected with MP and, at the same time, treated with erythromycin (treating group n=4,P＜0.05). Bacterial cultures of the bronchial and lung tissue were negative in all three groups. Using transmission electron microscope the widened interalveolar septa with increased amount of collagen were found in the infectious group, while there were no obvious abnormalities in the other two groups.

结果显示：(1)感染组动物(n=4)支气管肺泡灌洗液肺炎支原体-PCR检测均为阳性，而对照组(n=4)和感染加红霉素治疗组动物(n=4)均为阴性(P＜0.05)；三组动物的支气管和肺组织常规细菌培养结果均为阴性；感染组动物透射电镜检查见肺泡间隔增宽，其中有较多胶原纤维堆积，其余两组则未见明显异常。

Methods: Forty mice were divided into four groups randomly:normal group,model group,ligation group and treatment group.The model was made by the method of partly ligating the recta outside the body.The level of endotoxin in blood plasma was measured by dynamic nephelometric method and the content of TNF-a in blood serum was detected by the method of radioimmunity.(2)After the mice were killed,their left lungs were taken out for bronchi-alveoli fluid, then the content of MDA in BALF was tested by TBA method.(3)The vitality of PAM in BALF was countied on the counting set of erythrocyte,and the blue-dyed cellswere counted, then the percentage of PMN was worked out.(4)The pathological features of the mice\'s lung tissue were observed with routine and ultramicro pathological mechanism.

①将40只大鼠随机等分为正常组、模型组、解扎组、治疗组，以体外直肠不全结扎法造模，以动态浊度法检测各组大鼠血浆内毒素水平，放射免疫分析法检测各组血清TNF-α含量；②大鼠处死后，取左肺进行支气管肺泡灌洗，以TBA法测定支气管肺泡灌洗液中丙二醛含量、白蛋白含量及肺表面活性物质水平；③在红细胞计数盘上作BALF中肺泡巨噬细胞活力计数，计数兰染的细胞数，求出死亡百分率；在光镜下计数BALF沉渣200个细胞，计算中性粒细胞所占百分比。

Results:The following phenomena were observed:the Ph, PaO2 of the model rabbits descended sharply, the PaCO2 increased lightly, the HR slowed down obviously, but the BR increased, the SBP and DBP of the model rabbits descended markedly, and the PS content decreased dramatically. The pneumonic pathology changes showed pneumorrhagia, atelectasis, shrink of alveolus and pulmonary hyaline membrane. The above results indicated that it accorded with the standard of I-type of acute respiratory distress syndrome.

结果：整肺灌洗后模型兔的血气pH、PaO2急剧下降，PaCO2 轻度上升，心率明显减慢而呼吸频率增加，SBP和DBP均明显下降；支气管肺泡灌洗液中PS含量明显下降；病理检查提示出现肺出血、肺不张、肺泡萎缩、透明膜形成等病变，符合Ⅰ型重症呼吸衰竭临床诊断标准。

Methods: Twenty-four rabbits were randomly divided into three groups:① the control group(group C, n=8): received sternotomy only and no ischemia;② the ischemia/reperfusion group(group I/R, n=8): the left lungs of rabbits were rendered ischemia by ligating the left pulmonary hili for 60 minutes followed by 60 minutes reperfusion;③ the edaravone group(group E, n=8): the left lungs were rendered ischemia for 60 minutes followed by 60 minutes reperfusion, and 10 mg/kg edaravone was administered intravenously 5 minutes prior to ischemia. Blood MDA and SOD were measured. Protein concentrations, WBC count, and PMN percentage in broncho-alveolar lavage fluid were determined. Samples of left lung tissue were sent for determining the left lung dry-to-wet weight ratio and evaluating the pathologic changes.

采用兔肺原位热缺血再灌注损伤模型进行研究。24只大白兔随机分为三组：①对照组(C组， n=8)，开胸后不阻断肺门，静脉缓慢推注生理盐水5ml/kg；②缺血再灌注组(I/R组， n=8)，左肺接受60min的缺血，然后接受60min再灌注，于缺血前5min静脉缓慢推注生理盐水5ml/kg；③依达拉奉干预组(E组， n=8)，于缺血前5min静脉缓慢推注依达拉奉10mg/kg（5ml/kg），左肺接受60min的缺血，然后接受60min再灌注。120min实验结束时，留取各组动物血液标本，测定血浆丙二醛含量及超氧化物歧化酶活性；收集支气管肺泡灌洗液，检测支气管肺泡灌洗液中白细胞计数、中性粒细胞百分比及肺通透性指数；留取肺组织标本，测定肺组织湿、干重量，计算干／湿重比及行肺组织病理检查。

Fiberoptic brochoscope is a useful instrument to diagnose lung cancer. Bronchial lavages using fiberoptic bronchoscope could not only improve the diagnosis of lung cancer, but also cost little. In addition, bronchial lavages, called"fluid biopsy", is helpful to diagnose primary of secondary carcinoma locating in respiratory tract, including peripheral lung cancer, bronchioalveolar carcinoma, pulmonary metastatic carcinoma and lymphoma.

纤维支气管镜检查是诊断肺癌的有力武器，支气管灌洗不仅能稳定地提高肺癌检出率，而且价格便宜，此外，能研究常规支气管镜未见异常的肺部病变，有助于诊断呼吸道原发性或继发性恶性肿瘤，包括周围型肺癌，细支气管肺泡癌，肺转移癌，癌性淋巴管病，肺部淋巴瘤等，被称为&液相活检&。

After 1hour,4 and 6hours,bronchoalveolar lavage was done,and the function of PAMs,the amount of PAMs and the lung relative weight were determined.Results Lycopene could increase the phagocytic function of PAMs significantly .

以05，10，30mg/的番茄红素连续给雄性Wistar大鼠灌胃30d，用脂多糖制造大鼠急性肺损伤模型，分别在1，4，6h后支气管肺泡灌洗，检测肺泡巨噬细胞吞噬功能、细胞计数以及肺相对重等指标。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。