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The antibodies were purified and the purity and specificity were identified by SDS-PAGE and Western-blot,respectively.Lung tissues from five pigs with PRRS were tested with the established method,which clearly demonstrated the abundant presence of viral antigens in the cytoplasm of alveolar macrophages,and to less extent in alveolar and bronchiole epithelial cells.

SDS-PAGE和Western-blotting检测结果表明,制得的兔抗PRRSV IgG纯度较高,具有良好的特异性,可用于原位检测病猪肺组织中PRRSV抗原的分布;Envision法的检测结果显示,PRRSV抗原的阳性表达产物主要出现在肺巨噬细胞胞浆内,其次为肺泡上皮细胞和细支气管黏膜上皮细胞;通过对5份经RT-PCR检测确认的PRRS病猪肺组织进行检测,均有较高的阳性细胞表达,平均阳性细胞率为46.5%。

Ravan Napas can reduce the expression of ICAM 1 and HO 1 on broncho epithelial cells and thus inhibits the aggregation of the inflammatory cells and the release of the inflammatory media, as a result leads to less inflammation of bronchia. Such inhibitory way might be one of the possible mechanisms of Ravan Napas in treating asthma.

纳气平喘颗粒通过调节支气管上皮细胞ICAM 1和HO 1的表达,可抑制炎性细胞在气道内聚集及其炎性介质的释放,减少气道变应性炎症,这可能是纳气平喘颗粒治疗哮喘病的部分机制。

The Sprague-Dawley rats were intratracheally instilled with lipopolysaccharide(LPS,2 mg·kg-1 per day) for two days to induce acute lung injury.The rats were sacrificed at 72 hours after LPS instillation.Lung morphology was studied.

气管内注入LPS复制大鼠急性肺损伤模型,观察72 h后的病理变化、支气管肺泡灌洗液中细胞总数的变化、免疫组织化学特点以及蓝桉油的影响。

METHODS Low-dose Peth 0.01, 0.1 and 1 mgkg^(-1) were given iv 10 min before guinea pigs inhaled 75% cigarette smoke 60 mL. Then, the changes in airway resistance R(subscript aw, dynamic lung compliance C(subscript dyn and vascular permeability in airway tissues were observed. The total leukocyte and the differential leukocyte in bronchoalveolar lavage fluid and nitric oxid e content in BALF were investigated after guinea pigs given iv Peth 0.1 mgkg^(-1) 10 min before cigarette smoke inhalation and consecutively inhaled 360 mL smoke in 2 h.

观察Peth 0.01,0.1和1mgkg^(-1)对豚鼠自主吸入75%香烟烟雾(含25% O2)60mL后,气道阻力和肺动态顺应性变化的影响及气道组织血管通透性变化的影响;观察Peth 0.1mgkg^(-1)对豚鼠2h内分6次吸入(共360mL)75%浓度的香烟烟雾后,支气管肺泡灌洗液中白细胞总数和分类计数改变的影响,测定BALF中一氧化氮含量。

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