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Result The flexibility of the scaffold material was powerful. The load-elongation curve of the mechanical testing was similar to that of ACL, the maximum load, the ultimate stress and the Young's modulus of the scaffold materials were 52.61 N, 14.96 MPa and 202.08 MPa respectively. Human ACL cells displayed the representative characteristercs of the ligamentous fibroblasts, and synthesized extracellular matrix, such as type Ⅰand Ⅲ collagen protein. The scaffold materials had no cytotoxicity. Human ACL cells adhered, grew and proliferated well both on the surface and in the holes of the scaffold materials.

结果]韧带支架材料的柔韧性强,拉力测试的负荷-拉伸曲线与韧带的拉伸曲线相似,其最大负荷、极限应力和弹性模量分别为52.61N、14.96MPa和202.08MPa;体外分离培养的人前交叉韧带细胞呈典型的成纤维细胞特征,能在体外分泌Ⅰ、Ⅲ型胶原等细胞外基质;支架材料无细胞毒性,人前交叉韧带细胞可在支架材料上黏附、生长并分泌细胞外基质。

The obtained composite scaffold materials must have great biocompatibility, which could influence neuraxon regeneration and structure. An evaluation of permeability in varying molecules was a key factor to determine input of nutritive materials and output of metabolic waste.

不论用哪些材料,所制成的复合支架都要具有生物相容性,这在宏观和微观上都影响神经轴突再生的能力和结构;对不同大小分子的渗透性是评估营养物质进入支架和代谢废物排出的关键性能;要清楚支架在脊髓水环境中的膨胀程度,使其保持在合适的位置而不压迫再生的神经。

The obtained composite scaffold materials must have great biocompatibility, which could influence neuraxon regeneration and structure. An evaluation of permeability in varying molecules was a key factor to determine input of nutritive materials and output of metabolic waste. Expansion of scaffold should be understood under spinal cord water environment, because the expansion might press regenerated nerve.

不论用哪些材料,所制成的复合支架都要具有生物相容性,这在宏观和微观上都影响神经轴突再生的能力和结构;对不同大小分子的渗透性是评估营养物质进入支架和代谢废物排出的关键性能;要清楚支架在脊髓水环境中的膨胀程度,使其保持在合适的位置而不压迫再生的神经。

The iliac bone defect moulds were prepared and the compound scaffolds were implanted. The compound scaffolds were taken out after four and eight weeks separately, bone healing status and appearance of scaffolds were observed by eyes, the osteoplast growth status in compound scaffolds were viewed by HE and chinalizarin dyeing separately, the degradation of the compound scaffolds in vivo were observed by SEM.

制作髂骨骨缺损模型,在骨缺损处植入骨支架复合体,植入后4周、8周取材大体观察材料与周围骨愈合情况,HE染色及茜素红染色观察支架复合体内成骨细胞生长及扫描电镜观察支架体内降解情况。

According to the Wakitanis standard, the group A has the best plerosis effect on the repairing of articular cartilage defect compared with the other groups. It had been proved to have the significantly difference from all the control groups by statistics disposal.

按照Waki tani标准修复关节软骨效果由好至差的顺序为有生长因子BMP一2的细胞改良FG支架复合物组、无生长因子的细胞改良FG支架复合物组、单纯改良FG支架、空白对照组。

Trans-Blot Cells and Systems 170-3825 Trans-Blot Cell With Wire Electrodes and PowerPac HC Power Supply, 100120/220240 V, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm), power supply, power cord, instructions 170-3850 Trans-Blot System With Plate Electrodes and PowerPac HC Power Supply, 100120/220240 V 170-3853 Trans-Blot System With Plate Electrodes, Super Cooling Coil, and PowerPac HC Power Supply, 100120/220240 V, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm), power supply, power cord, instructions 170-3910 Trans-Blot Cell With Wire Electrodes, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm) 170-3939 Trans-Blot Cell With Plate Electrodes and Super Cooling Coil, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm) 170-3946 Trans-Blot Cell With Plate Electrodes, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm) Trans-Blot Cell Accessories 170-3912 Super Cooling Coil, required for all high-intensity transfers 170-3913 Gel Holder Cassette, includes 2 fiber pads 170-3914 Fiber Pads, 15.5 x 20.5 cm, 6 170-3920 Trans-Blot Standard Wire Electrode Card, cathode 170-3921 Trans-Blot Standard Wire Electrode Card, anode 170-3922 Trans-Blot Cell Buffer Tank 170-3923 Trans-Blot Cell Lid With Power Cables 170-3943 Trans-Blot Platinum Anode Plate Electrode 170-3944 Trans-Blot Stainless-Steel Cathode Plate Electrode 170-3945 Trans-Blot Plate Electrode Pair, platinum anode and stainless-steel cathode 16 规格:说明: Trans-Blot Plus

电泳转印槽组件 1。缓冲液槽及带有电缆的盖 2。凝胶支架转印夹 3。纤维衬垫 4。电极丝 5。电极板 6。特级冷却芯 Trans-Blot 转印槽是功能灵活的转印设备,可理想地用于多种转印应用。Trans-Blot 转印槽特点包括:*能进行多胶转印,可容纳3 块PROTEAN I xi 凝胶、6块Criterion 凝胶、12块Mini-PROTEAN 3 或Ready Gel 预制胶*多组参数灵活可设,可调节的电压设置(从30 V 的过夜转印到200 V 的1 小时快速实验)*电极间距设置为8 cm 用于标准印迹杂交,或设置为4cm 用于高强度印迹杂交*可选择板式电极:涂有铂金的钛作为正极,不锈钢为负极,能提供高强度电场和比其它电极更高的电流密度。或选择较经济的铂金电极丝*通过特级冷却芯和水循环仪来调节温度―是天然酶(4°C)或高强度转印的理想选择,随着转印时间增加(多达24 小时),不会引起缓冲液耗竭(在高强度转印中必须使用冷却芯,也推荐用于所有板式电极的应用)*带铰链的凝胶支架转印夹能避免滑动,确保凝胶与印迹膜间的紧密接触;每个转印夹都有颜色标记以保证在转印槽中的正确定位 Trans-Blot 转印槽的锁闭凝胶支架转印夹系统。转印夹(1)支撑凝胶(2)印迹膜(3)两侧有纤维衬垫和滤纸(4)确保凝胶三明治内的完全接触。凝胶夹垂直插入缓冲液槽中(5)。

Their results showed a "sustained reduction in the need for reintervention after the use of sirolimus-eluting stents," and a risk of stent thrombosis no lower than observed with bare-metal stents.

结果表明&使用西罗莫司洗脱支架后介入治疗的需求持续减少&,并且和使用裸露的金属支架相比,病人发生支架血栓的风险性也不低。

The amount of neuron at 2st week for EG and CC were 36.50±1.78/EF and 19.70±3.71/EF respectively, with the percentage of (43.60±4.83)% and (39.04±3.87)% for EG and CG, which had significant difference (P.05) Conclusion: Self-assembled IKVAV peptide nanofibers, which can degrade the neuron mortality as well as promote neurite sprouting and growth, is a bioactivity scaffold, it can serve as a superordinary nerve tissue engineering scaffold.

自组装IKVAV多肽纳米支架能降低神经元的死亡率,并诱导轴突的发生和生长,具有支架及生物活性双重作用,可作为神经组织工程支架材料。

DATA SYNTHESIS:①Based on the material, tracheobronchial stents are generally of two types, metallic and non-metallic.

资料综合:①根据材质,气管内支架可分为金属支架和非金属支架两种。

The design and fabrication of bone tissue engineering scaffolds are crucial for the study of thermodynamics and Dynamic mechanical performance ofβ-tricalcium phosphate.

本项研究从骨组织工程支架设计与制造入手,研究β-磷酸三钙/聚乳酸复合支架材料的热力学性能和动态热机械性能,寻求最合适支架作为骨组织替代物。

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