操作结束

The fundi were irradiated with a energy fluence of 20, 30 and 45 J/cm within 5 minutes after the drug injection of 2.5, 5 and 10 mg/kg.

光斑功率密度200、300和450mW／cm~2，能量密度20、30和45J／cm~2，分别在注射光敏剂结束后10s、5、10和15min行PDT操作，每组PDT方案10个光斑。

Animation is based on simplemotion tween concept: set-up start and end look of objects and Giotto does the rest by itself.

动画基于简单的动作理念：设置开始和结束点对象的外观，然后 Giotto 自己处理其余的操作。

After inserting the start point, you need to insert the end point at the end of definingtransaction operation.

插入事务的开始点后，在需要定义事务的操作后面插入事务的"结束点"。

This kind of operation led to the low quality works and it nearly drew Ukiyo-e prints to its end fifty years in advance. Fortunately, Katsushika Hokusai and Utagawa Hiroshiga save this backwardness of Ukiyo-e prints.

浮世绘的商业操作模式，导致了一些作品的低俗粗陋和模仿抄袭之风，这类不良品质差点提前五十年结束了浮世绘的生命，幸亏葛饰北斋和歌川广重的出现给了浮世绘一次挽救。

Brand manager Pavla Vernerova of Soma said: 'We are keeping the specification of the new Soma Flex Imperia under wraps until drupa where it will be shown in operation running live printing jobs.

品牌经理pavla维尔内罗娃的索马说：'我们正保持规格的新索马flex的因佩里亚下结束，直到德鲁巴的地方将显示在操作运行Live印刷工作。

The nonsolvent process of the preparation for oxamyl is suitable for mass production due to its low production cost, high purity, no methyl isocyante remains, easy operation and lower equipment cost.

水相法合成杀线威不需催化剂和溶剂，产品制造成本低，纯度高，反应结束异氰酸甲酯无残留，出料环境好，操作方便，设备投资少，是一种适合大生产的工艺方法

Over the past few years, we have adhered to the margins pilot, while summing up the experience in the dark improve, and we invite the four mapping units, according to the "phased implementation of the subregional mapping and cadastral mapping project, a map, several related databases" for the pilot, and gradually establish and perfect the mapping system : First, develop Fuzhou mapping management, Fuzhou administrative districts in operational and management activities in the real estate mapping units and individuals, thus greatly enhancing the management of our real estate mapping, mapping for the development of the cause.

为加强房地产产权产籍管理走出了一条新路。我市房产测绘体制改革是伴随着社会主义市场经济的建立和发展逐步铺开的。80年代中期，我市房屋产权登记开始恢复时，尚未建立专业的测绘队伍，在房屋产权确认中作为重要依据的图、档以及房屋面积都是由产权登记人员用皮尺到现场手工丈量确定。1985年房屋普查结束后，原普查人员组建成市测绘处，负责全市房产测绘工作，人员经简单培训后上岗，测量停留在手工皮尺操作上，技术没有规范，人员缺乏专业知识。

It is impossible for the repository to end up in a state with only half of the operations done.

在配置库中操作进行了一半的时候就结束是不可能的。

The method has the advantages that: the high-concentration substrate inhibition is eliminated and nutritive materials are also supplemented through controlling the pH value in the fermentation environment and supplementing a carbon source and the substrate, so the thalli can grow in the suitable environment, the content of phenyllactic acid reaches 6 to 20g/L after the fermentation, the process is simple, the environmental pollution is less, the operation is convenient, and the output of phenyllactic acid is high.

本发明的优点是：通过控制发酵环境的pH，并补加碳源和底物，既解除了高浓度的底物抑制，又补充了营养物质，使得菌体能够在适宜的环境下生长，发酵结束后，苯乳酸的含量达到6～20g/L，工艺简单、环境污染小、操作方便、苯乳酸的产量高。

Part II The experiment study on anti-tumour effect of dendritic cells derived from peripheral blood monocyte of patients with oral cancer after pulsed by tumour antigen in different ways. Collected 50ml peripheral blood from each patients and volunteers to induced DC in vitro. We parted the test group and control group into three subgroups respectively, untreated group A, freezed and thawn group B, RNA transfected group C. To group A, after induced DCs for five days , we added 200U/mlTNF-α in each hole and cultured on. To group B, we added protein antigen (DC/ tumor cells = 1 : 1 ,each hole) for 4h at 5d, and then added 200U/mlTNF-α in each hole and cultured on. To group C, transfected immature DC with tumor cell RNA(DC:RNA=1×105/ml: 5ug)at 5d, operated completely with the specification of invitrogen DMRIE-C reagents. After transfection we replaced transfection agent with complete medium, and then added 200U/mlTNF-α in each hole and cultured on.

第二部分口腔癌患者外周血来源的树突状细胞体外负载抗原后抑瘤作用的实验研究无菌采集20例口腔癌患者和20例健康志愿者外周血50ml，分离单个核细胞体外诱导培养DC，并将实验组和对照组细胞各分为三组，A组为未处理组，B组为冻融组，C组为RNA转染组。A组DC在培养5d后，每孔加入TNF-α200U/ml；B组DC培养第5d后，每孔中加入癌细胞蛋白抗原，使DC/肿瘤细胞=1：1，继续培养4h后，每孔加入TNF-α200U/ml；C组将培养至第5d的未成熟DC用肿瘤细胞RNA进行转染(DC：RNA=1×105/ml： 5ug，按照invitrogen DMRIE-C试剂说明书进行操作)，转染结束后用完全培基取代转染剂，每孔加入TNF-α200U/ml。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了