摸索

The inclusion body of IFN-BLA was denatured and refolded by dialysis and purified by ion-exchange chromatography. The overall yield of IFN-BLA was about 45 mg/L with purity higher than 90%.

对包涵体的复性条件进行了摸索，建立了IFN-BLA的复性及纯化方法，纯化后的蛋白产量约为45 mg/L，纯度在90％以上。

Kneeling down beside the trunk again, he groped around in the bottom and, after retrieving an old badge that flickered feebly between SUPPORT CEDRIC DIGGORY and POTTER STINKS, a cracked and worn-out Sneakoscope, and a gold locket inside which a note signed R.A.B.

当哈利再次跪在箱子边，摸索着箱子的底部时，他找到了一个两面闪烁着&支持塞德里克·迪戈里&和&波特臭大粪&的发光的徽章、一个裂开的窥镜，还有一个金色小盒子，里面藏着那张署名为R·A·B的纸条。

Unmistakeable sound of a man rummaging down his trousers...

一阵不容置疑的一个男人在裤子里摸索的声音）（画外音：他不会真脱了裤子察看内裤吧……）蓝色。

Nigra during the experiment. Successfully established the transformation procedure Agrobacterium-mediated of Populus simonii×P nigra: cut vigorous leaves to 2-3 patches through the main vein after growing about 20 days, pre-culture time was 3~4days;centrifuge Agrobacterium when being developped to the logarithm growth timeOD_(600=0.5, then dilute Agrobacterium to the concentration of OD_(600)=0.3-0.4 with MS liquid culture medium or asepsis distilled water for infection;infection time was 6-15 minute;after being co-cultured 3 days, inoculate on the selecting medium to be filtrated in succession during inducing unsureness buds and rooting with Kanamycin(containing Kanamycin 40mg/L and Cefazolin Sodium 700mg/L).

成功摸索出农杆菌介导的小黑杨遗传转化体系：选取生长20d左右的健壮的小黑杨组培苗叶片，通过主叶脉剪2～3下，预培养3～4d；将培养至对数生长期的农杆菌OD_(600=0.5离心后用液体MS培养基或无菌蒸馏水稀释成OD_(600)=0.3～0.4浓度，进行浸染；侵染时间为6～15min；共培养3d后，转入到筛选培养基中进行诱导不定芽及生根阶段的卡那霉素连续筛选(含有40mg/L卡那霉素和700mg/L的头孢唑林钠)。

On the basis of many experiments and many systematically tests of various ventilators, one reliable calibration method has been established for ventilators, which is scientific, reasonable, and easy to use.

通过长期的实践摸索，我们对各种呼吸机进行了系统的测试和论证，初步建立了一套可靠的校准方法。

The most distinctive feature of the system is use of simple and inexpensive two-phase closed thermosyphon as the primary heat pipes.

为验证其原理上的可行性，作者分别进行了单管和配机的实验研究，并对多种制造工艺进行了摸索。

In order to exploit the application of modern design method to fan design, this paper discussed the CFD calculation and groped the theory and practice of CAA.

为了探讨现代设计方法在冷却风扇设计中的应用，本文对冷却风扇的计算流体动力学问题作了一定的探讨，同时，也对计算声学的理论与实践作了一定的摸索。

Ok Karen, Bourdain gets a good grope of you in your V neck sweater, and we get hotdogs and teases…...

好吧卡伦，伯尔顿获取你的V领毛衣的你身体摸索，我们得到热狗和开玩笑。。。。。

When the reaction was carried out under the above optimum conditions, the conversion of mandelonitrile was 42 % and e.e.

在此基础上合成氰醇类底物，运用摸索的条件对其进行动力学拆分，并探讨了芳环上的取代基R对反应结果的影响。

Now, the 2008 Beijing Olympic Games, the 2010 Shanghai Huambo has decided that China will continue to be the dominant world economy, the capital Beijing is a beautiful future, our group has also been exploring and seeking to enter Beijing opportunities.

如今，2008年北京奥运，2010年的上海万博已经决定，中国将继续成为世界经济的主导，而首都北京便是拥有无限美好的未来，我们集团也一直在摸索，寻求着进军北京的商机。

I will endeavour to find you some assistance.

我尽力帮你找人帮忙。

At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。