搜索过程

Total RNAs from KAx-3 cells and AK127 cells(developed for 14h) were isolated. After the reverse transcription and PCR reaction, two distinct differential fragments were acquired., fragment A was from KAx-3 cells and fragment C was from AK127 cells. After retriving and reamplifying the differentially expressed fragments, white-blue plaqueselection, the fragments were purified. Northern blot proved that fragment A was from KAx-3 cells and fragment C was from AK127 cells. The results of sequencing and researching for NCBI database have been showed: part sequence of fragment A shows 91% similarity to the gene encoding DhkA, 92% similarity to the gene encoding DhkF, 91% similarity to the gene encoding STATc, 97% similarity to the homoeobox gene encoding protein. These genes play important part in controlling cell differentiation and cell proportion in Dictyostelium discoideum.

本研究通过提取盘基网柄菌发育14小时的野生型KAx-3细胞和突变型AK127细胞的总RNA，运用mRNA差异显示法分离出了两条明显的差异表达片段，其中片段A来自野生型KAX-3细胞，片段C来自突变型Ak127细胞；并通过凝胶回收差异片段、对差异片段进行再次PCR、蓝白斑筛选克隆、提取质粒、酶切电泳纯化差异片段；接着进行Northern杂交的结果表明，片段A只与野生型KAx-3细胞的总RNA有杂交信号，片段C只与突变型AK127细胞的总RNA有杂交信号，这就排除了差异片段假阳性的可能；最后通过测序，搜索NCBI BLAST数据库发现：片段A的小部分序列与编码组氨酸激酶DhkA基因中一段序列的相似性高达91%，与编码组氨酸激酶DhkF基因中的一段序列相似性高达92%，与编码STATc蛋白基因的一段序列相似性达91%，以及与编码同源框蛋白的基因中的一段序列相似性达97%，这些基因在盘基网柄菌细胞分化和细胞比例调控过程中起着相当重要的作用，这些数据进一步说明了突变细胞不能完成发育的原因。

During the procedure for searching k nearest neighbors of tested sample, the particle swarm moves jumpily, and those document vectors that are impossible to be the k closest vectors are kicked out quickly.

在搜索k近邻的过程中，粒子群跳跃式移动，掠过大量不可能成为k近邻的文档向量，从而可以快速找到测试样本的k个近邻。

During the procedure for searching K nearest neighbors of tested sample, the particle swarm move jumpily and randomly, and save largely the classification time. PSOKNN has the same classification performance as that of KNN classification algorithm.

PSOKNN算法在搜索K近邻的过程中，粒子群跳跃式移动，可以快速的找到测试样本的K个近邻，使得在保持K近邻法分类性能不变的情况下，分类的效率得到很大的提高。

The method is applied to the system reliability assessment of a long-span cable-stayed bridge.

在β约界法搜索失效模式的过程中，需计算各个单元的可靠度指标。

Want to had made the rank in this process, the search when wanting basically to be being searched gets assuring a lot of people to come in, as the forum sending a station of game, also be in slowly development.

在这个过程中要把排名做好，基本要在搜索时搜得到保证很多人进来，随着游戏的发站论坛，也在慢慢的发展。

During this lecture Chen surprised us a lot. He told us what the little world theory was and he introduced application of it in the Internet. Such as Web2.0, self-evolution of the Internet, communication net , searching and so on.

整个演讲过程中陈争光同学给我们介绍了什么是小世界理论，以及小世界理论在互联网世界中的应用，例如 Web2.0、网络的自我进化、社交网络以及搜索等等。

The method of obtaining time delay parameters with the reverse access channel is discussed. The modified algorithms of classic correlation methods of TOA estimation are proposed. Noncoherently combining algorithm and group coherent combining algorithm are given. They decrease the computational complexity and are more suitable for implementations in practical systems. The leading edge detection algorithm is proposed to mitigate the effect of multipath in TOA estimation. Then the secondary search method is proposed, it can solve the matching issue of PN code in CDMA systems. The location algorithms based on LS are discussed; include circular trilateration with LS, hyperbolic trilateration with reference differencing, hyperbolic trilateration with sequential differencing and circular trilateration with TOA differences. The design and implementation of wireless location system based on TOA/TDOA in CDMA2000 systems are given. The location process is discussed, and the hardware and software implementation of TOA estimation are given. Finally, the wireless location system which uses TOA estimation and LS location algorithms above is tested in the real environment.

文中讨论了采用CDMA2000反向链路中的接入信道来获取时延的方法和过程；对TOA估计的传统的相关法进行了改进，提出了非相干合并相关法和分组合并相关法，减少了基于相关的时延估计算法的运算量，提高了算法的实用性，对算法进行了仿真和性能分析；采用边缘检测技术有效减少了TOA参数估计中的多径干扰，提高了时延参数估计的准确度；提出了一种TOA估计的二次搜索法，解决了多个定位测量单元同时进行TOA估计的PN码匹配问题，进一步了提高TOA估计的精度；在采用时延值的定位算法中引入了基于LS的圆周法、基于参考差的双曲线法、基于相邻差的双曲线法和基于TDOA的圆周法，讨论了各算法的应用条件，进行了算法性能的仿真分析；给出了CDMA2000系统中的TOA/TDOA的无线定位的系统方案和实现流程，并对TOA值估计算法进行了软硬件实现，还对采用TOA估计算法、LS定位算法和定位系统设计方案实现的定位系统的进行了现场定位性能测试。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。