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A human transforming growth 〓 cDNA was inserte in reverse orientation into the retroviral vector, pLXSN. Restriction en zyme analysis was used to confirm the antisense orientation of 〓〓 in the pLXSN vector from individual transfomants.

将人的转化生长因子-〓cDNA(1467bp)反向插入逆转录病毒载体pLXSN,筛选转化菌,挑取抗性克隆,用限制性内切酶酶切鉴定重组子。

Abjective to express the Hepatitis A virus genome cDNA attenuated salmonella Typhimurium and to find the immunity to hepatitis A virus after administrated that salmonella Typhimurium with hepatitis A virus gemome to the mousehepatitis A virus coding region cDNA were inserted expressive plasmid PBV221 and then were transform into E coli DH5α.

使甲型肝炎病毒基因组cDNA能够在减毒伤寒杆菌中表达,从而通过口服此种减毒伤寒杆菌达到对甲肝免疫的作用。将甲肝病毒编码区cDNA插入高效表达质粒PBV221,转化大肠杆菌DH5α,筛选并鉴定阳性克隆。

A portable target stand having one or more targets mounted on a target backer which is adjustably supported by two vertical support posts partially inserted into the ground.

一种便携式靶子支架,具有一个或多个靶子固定于一个靶子垫座之上,该靶子垫座由两根竖直方向的,一端部分插入地面的支撑杆支撑,并且其高度可调。

Background: Abduction weakness and limping is a well-recognized complication of closed antegrade insertion of femoral nails.

研究背景:外展无力和跛行是闭合顺行插入股骨髓内钉普遍公认的并发症。

Higher concentration of barbaloin could convert DNA configuration of hepatoma carcinoma cell Bel?

结果与结论芦荟大黄素以插入方式与DNA分子结合;较高浓度的芦荟大黄素苷能够使Bel?

Corner of the eye opening is the largest plastic surgery under local anesthesia downlink outer canthal angle cut, cut the length according to the need to enlarge the extent of blepharophimosis with blunt scissors inserted from the incision in the conjunctiva stealth testimony was stripped.

1眼角开大整形手术是在局麻下行外眦角切开,切口长短根据睑裂需要放大的程度,用钝头剪刀从切口插入,在球结膜下作潜行剥离。

PCR , Southern blotting and selection on kanamycin medium indicated that BoCAL gene had single-sited integrated into cauliflower and conformed to the segregation of single-dominant gene by the law of Mendelian Inheitance.

PCR、Southern杂交和抗卡那霉素筛选表明,外源BoCAL基因已经转入受体植物并且是单位点插入,并且符合孟德尔单显性基因的遗传。

Methods We used the automatic DNA sequencing machine (Model 377) to detect the nucleotide sequence of the inserted part of the recombinant plasmid pBX1 from Borrelia burgdorferi B31 strain.

方法采用377型DNA自动测序仪对莱姆病螺旋体重组质粒pBX1的插入片段进行DNA序列测定,并通过计算机软件对其进行限制性内切酶酶谱分析。

A confluence between the internal jugular ein and the brachiocephalic ein facilitates cannulation at this location.

在这里,颈内静脉和无名静脉的汇合使套管插入更容易。

Early 16th-century Franco-Flemings moved away from the complex systems of canonic and other mensural play of Ockeghem's generation, tending toward points of imitation and duet or trio sections within an overall texture that grew to five and sic voices.

他们也开始,甚至在宗教改革之前,插入了比以往任何时期都要长的主调段落,以强调重点部分或衔接点。

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The objective is to subjugate and discourage the people, because that allows the elite to continue to rule unopposed.

其目的是压制和打击人民的积极性,因为这可以让实权派继续统治不会沦为反对派。

GOD,this is the second time you vanquished me!

天啊,这是第二次你打败了我!

So a kind of strong antagonism permeated in the relations of two countries.

所以两国这一时期的政治关系始终弥漫着一种强烈的对抗情绪。