插入的

If the caret appears anywhere else, it is simply regarded as a caret with no special meaning.

如果插入符号出现其他地方，它只是被视为插入符号，没有特殊的含义。

Because positive clones utilizing nitrate or nitrite as sole nitrogen source werentdirectly isolated from the genomic library, random miniTn5 insertion mutant library wasconstructed via biparental conjugational transfer between E.

由于不能从文库直接筛选到利用硝酸盐的阳性克隆，因此，通过Tn5插入突变构建了随机插入突变库，采用E。

Clicking on the Insert Column icon on the Table toolbar inserts a column after the selected one.

点击工具栏上的插入列图标，在表中选定列后插入一列。

Clicking on the Insert Row icon on the Table toolbar inserts one row below the selected one.

点击工具栏上插入行图标，在表中选中行的下面插入一行。

If you use the Insert Footnote Directly or Insert Endnote Directly icon, the footnote or endnote automatically takes on the attributes previously defined in the Footnote Settings dialog box .

如果您使用直接插入脚注或直接插入尾注图标，脚注或尾注自动采用之前在脚注设置对话框里定义的属性。

In Microsoft Word 2007, users can insert Lorum Ipsum Latin text by simply typing "=lorem" follow by Enter in the document, with n determines number of paragraphs that will be inserted, and n can be null mean nothing is insert between bracket, ie (, or 1, 2 and other number.

在Microsoft Word 2007中，用户可以插入lorum ipsum拉丁美洲的文字简单地输入"= lorem的"的后续由输入在文件中，与N确定的一些段落，将插入，和N可以空（毫无意义的是插入之间的支架，即，或1， 2及其他号码）。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

On the safe side, we should use active soil pressure and passive soil pressure to calculate the support strength before T increased. That is RA=EA-EP Among them: RA ………… holding strength EA ………… composite force of active soil pressure EP ………… composite force of passive soil pressure Immediately, flexural torque distribution of enclosure wall can be counted. Mmax is the location of shear force zero. Equivalence beam method abridges. No matter which kind of supporting structure form, the key problem lies in the establishment depth of catch point, severity of support and enclosure wall, penetration depth and pit bottom quality of soil property.

单道锚杆支护结构计算分别采用静力平衡法及等值梁法，根据试算法插入深度t值需先进行假定墙的插入深度确定后来计算支撑力，为了安全起见，可按插入深度增大前的主动与被动土压力合力计算支撑力即 RA=EA-EP 式中：RA …………支撑力 EA …………主动土压力合力 EP …………被动土压力合力随之可求围护墙的弯矩分布，Mmax为剪力为零点的位置，等值梁法的计算略述。

The results indicate that the relative atom content of Nd islow contrasted to that of Fe, which results from the weak competitive reaction ofNdCl〓 because of the strong bulk effects. The results of x ray photoelectronspectroscopy reveal that other valence of Fe could be in NdCl〓-FeCl〓-GIC.It is considered as the result of the charge shift between FeCl〓,〓 and graphitein intercalating process. Structural model of RECGIC was supplemented after thinking of the chargeshift between the intercalates and graphite. The characteristic layer distance, I〓, ofstage-1 RECGIC were calculated by the model.

利用能谱分析测定了NdCl〓-FeCl〓-GIC中插入剂的含量，结果表明， Nd的相对含量低于Fe的相对含量，这是因为NdCl〓的空间效应较大，而致使其竞争插入反应处于劣势的缘故。X光电子能谱研究表明，Fe可能有其它价态存在，这是由于在插入反应过程中，FeCl〓、NdCl〓以及石墨间发生电荷转移等作用的结果。

The authors give the definition of insertion loss of electric locomotive in inductive communication through trolley wire in a mine tunnel and compute insertion loss and transmission loss. After contrasting the two losses, we know that the insertion loss is very large and it is necessary to use dedicated induction line.

给出了在矿井中利用电机车架空线进行感应通信时，架空线负载引起的插入损耗的定义，计算了插入损耗和无负载时的电磁波传输损耗并对比了这两种损耗，可知插入损耗是非常大的，这说明了使用专用感应线的必要性。

It seems likely that Kraft will return with a better offer.

看起来卡夫要重新考虑一个更好的出价了。

But he'd rather burn off my feelers and watch me squirm.

可他却放任那个家伙对我为所欲为！