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In order to search for a suitable method for the tissue preservation and DNA isolation of marine animals, the adductor muscle of bay scallop was treated with different methods: frozen at -20 ℃, fixed with 70% ethanol and 10% formaldehyde more than 10 days.

以海湾扇贝为材料,研究了鲜活样品以及采用冷冻保存、70 %乙醇固定和10 %福尔马林固定等方法保存的闭壳肌样品用于DNA提取的不同效果,并分析了不同保存条件下所提得DNA用于RAPD扩增的结果。

Methods There were 26 patients(52 eyes) who all have accepted the treatment with resection of the superior obicularis muscle, repair for the aponeurosis of levator muscle and plasty of lateral canthus.

2003年8月~2005年8月共收治26例(52眼)眼睑痉挛的患者,均行上睑轮匝肌大部切除、提上睑肌腱膜修补、外眦成形等局部三联手术治疗。

The operation on the skin and the conjunctiva was performed without using microscope, the dermis and the aponeurosis of the levator muscle of upper eyelid were limited within the ring of the ligature by the line of suture ligatured to form firmly heal which keeps the formed double-fold eyeli...

其法的特征是皮肤面和结膜面均在直视下操作,增加了可信度,缝线结扎后保证了将皮肤的真皮层和提上睑肌的腱膜层均拦在结扎线环内,形成了牢固的粘连愈合,使之形成的重睑沟牢固持久,永不消失。

To examine the microvascular actions of tanshinone IIA researchers applied it topically to the hamsters' cheek pouch or mice cremaster muscles to achieve the final concentration of one mcg/ml or five mcg/ml.

研究微血管行动丹参酮IIA研究者应用于外用向仓鼠'颊囊或小鼠提睾肌,以达到最终的浓度一mg / ml时或5 mg / ml时。

AIM To study apoptotic gene expression of the endothelial cells in cremaster ischemia-reperfusion of the rat.

目的 研究大白鼠提睾肌缺血再灌后血管内皮细胞的凋亡基因表达。

METHODS: Nine cases(12 eyes), male 4 cases (5 eyes), female 5 cases (7 eyes), suffering from posteriority ptosis , 1/2 1/3 pupil covered by upper palpebral margin, the upper eyelid muscle strength more than 10mm, accompanied by the orbital zone depression with the upper eyelid fold increased. Neostigmine test were used to exclude myasthenia gravis.

本组病例共9例12眼,男4例5眼,女5例7眼,均表现为后天性上睑下垂,上睑缘遮盖1/2~1/3瞳孔,提上睑肌肌力均在10mm以上,伴有上眶区凹陷,上睑皱壁增高,部分患者行新斯的明试验排除重症肌无力。

In addition to zygomatic arch ligament observation, mandibular ligament, ligament platysma ears, before the ligament platysma skin, SMAS-zygomatic cheek cruciate ligament and the mandible, in the mandibular angle, lateral numbness of the mouth, etc.

除观察致颧弓韧带、下颌骨韧带、颈阔肌耳韧带、颈阔肌皮肤前韧带、SMAS-颧颊韧带和下颌骨韧带外,在下颌角、口角外侧、外眦外侧等处亦有皮肤支持带存在结论面部皮肤支持带粗、细不等,长、短不一,除皱术中需剪断和重建某些韧带,以达到最大程度的松解和提紧效果。

After staining with Congo Red,the absorption spectrum of the glyceri- hated toad sartorius muscle and honey bee indirect flight muscle is slightly different from that of the Congo Red solution.

本文报告甘油抽提的蟾蜍缝匠肌和蜜蜂间接飞翔肌经刚果红染色后的二向色性。

This study was conducted to examiune the fibrotic effect of Ni-Ti and 317L al loys in esophagus.The extract fluid from Ni-Ti,317L alloys was made according t o the ASTM standards of U.S.A. The Fb of esophageal scar was cultured primarily ,then incubated with alloy abstract fluid. The proliferating activity of Fb was measured by MTT at 4, 24, 48, 72 hours in the course of culturing. The esophagu s embedding test of Ni-Ti,317L alloys was made according to ASTM standards of U .S.A.The tissue around the alloys was taken at weeks 2 and 12,and the pathologi c changes were analysed.

为探讨新型支架材料Ni-Ti、317L合金在食管局部的致纤维化作用,按美国ASTM标准制备NiTi、317L合金的金属浸提液;&组织块培养法&原代培养食管壁疤痕的成纤维细胞,传代后以金属浸提液进行培养,分组后分别培养4、24、48、72 h,MTT法检测不同培养时间后Fb增殖功能的变化;按美国ASTM标准进行NiTi、317L合金试件的食管壁内包埋实验,即将金属试件经表面处理后直接置入食管壁粘膜层与肌层之间,术后2、12周取出包埋组织,分析试件周围组织的病理变化,并进行胶原纤维染色,观察纤维形成状况。

After overcoming the flux shift during the process, the production ability of strains could be the limiting factor for further increasing the production. To avoid the random modification on strains, the comprehensive enzymatic study of nucleoside producing-pathway was conducted on four strains with different producing ability. And the results could be used to direct further reconstruction of strains. It was also found that nucleotidase was of much importance to nucleoside production.

在成功克服了代谢流的迁移之后,菌株的代谢能力可能成为了进一步提高产率的限制因素,为了避免对生产菌的进一步改造中的盲目性,对四株不同菌株间嘌呤途径关键酶活力的系统测定,对肌苷、鸟苷生产菌种的酶学特征有了全面的了解;并发现了核苷降解酶对于核苷生产能力至关重要;酶学特性研究对肌苷、鸟苷生产菌的进一步改造提供了方向。

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐,因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。

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关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。