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The recombinant eukaryotic expression vector pcDNA3.1-Myc-His B/tPA can stable express tPAcDNA in transfected hUVEC cell strains and can secret compt tPA protein. Experiment III The Effects of Transfected tPA Gene on Vascular Smooth Muscle Cells Proliferation and Migration in VitroObjective To investigate the effects of transfected tPA gene on vascular smooth muscle cell proliferation and migration in vitro and to provide a basis for further study on preventing vein graft restenosis.

结论构建的pcDNA3.1-Myc-HisB/tPA基因新型真核表达载体，能在HUVEC中表达，其表达具有高拷贝、稳定性好的特点，获得的表达蛋白活性较高，提示本载体和稳定表达tPA基因的人脐静脉内皮株能够作为基因治疗工具用于哺乳动物的实验研究。

If this the first time you are seen this stop error screen restart your compter.

如果你是第一次看到这个错误提示窗口重新启动你的电脑。

From Conan's words, Jodie got a hint, and now knows everything that's happened.

从柯南的言词，，朱蒂得到了提示，并且现在知道一切的发生了。

Above all oneself should have popularity, can become one with with the colleague; the first pace makes acoustical call to the often buys bit of check again fellow with concern nicer with oneself first, there is bit of card to sell here, tell them to the dot blocks what price (the result that before this can be used, investigates, what colleague often gets stuck with what, tell him; compares the petty gain outside commonly straight from the shoulder some the) that they can buy, say to be clear about to there is what advantage here with them, for instance; can give card first close, the word that; economy actual strength pays to allow after is OK also lunar form, the time that if the metropolis that likes to play game more very much buys card suddenly when just sending salary to fill; to implement month of form, decides settle accounts date to the 2-3 day after sending salary (little hint: Lunar form is not frivolous do, want those who have a choice to do, often buy check with what won't the colleague of bilk does)!

首先自己要有人缘、能跟同事打成一片；第一步先向跟自己关系较好的又经常买点卡的同事打声招呼，自己这里有点卡出售，告诉他们什么点卡什么价格(这就可以用到之前调查的结果了，哪些同事经常用哪些卡，直截了当的告诉他；一般比外面的便宜些他们都会购买的)，跟他们说清楚自己这里都有哪些优势，比如；可以先给卡密，后付钱；经济实力答应的话也可以月结，很多喜欢玩游戏的都会在刚刚发了工资的时候猛买卡充；实行月结的话就把结算日期定到发了工资后的2-3天的时间(小提示：月结不要随随便便的都做，要有选择的去做，跟哪些经常买卡又不会赖帐的同事做)！

A549 cells are more sensitive to CDDP-induced apoptosis(P.01) and less sensitive to THP andβ-Ele-induced apoptosis than A375p(P.01, P.05 respectively). Condensation and crack of nucleus and apoptotic bodies appeared in apoptotic cells of A549 and A375p cell lines in all treated groups and necrocytosis were to be seen in some groups. Fluorescence imaging experiments demonstrated that accumulation of iASPP in cytoplasm but little distribution in nucleus in all groups. untreated groups in two cell lines presented a bright-green colour,followed by CDDP-treated groups,and THP-treated groups is the darkest one in all groups.

免疫荧光显示，未加药物处理的A549和A375p细胞胞质染色均匀呈亮绿色，核区较暗；CDDP及β-Ele处理组细胞为暗绿色，其中可见皱缩的凋亡细胞；THP处理的细胞胞质荧光弱呈灰绿色，由于THP嵌入DNA自发红色荧光与二抗的绿色荧光中和，胞核被染成桔红色，结果提示药物处理后A549和A375p细胞中iASPP的表达有不同程度的下降。

I want to eat some shave ice covered with lots of condensed milk.

提示1：译典可返回英文单词的不同语法变体，如果你不需要变体，请在查询词前加引号。

Right before the line the conditional jump at

在004201C5这行右侧的条件跳转提示我们

The meeting conveningDialing in the system, the convener, imports to relevant information and called out by the systemConferees join the meetingWhile conferee joining the meeting, he can inputs his individual message voluntarily according to system pronunciation so that system report to other conferees or host while he wants to make a speech or other things.

与会成员加入会议与会成员主动加入会议时，可以按照系统提示语音录入个人信息，以便在其请求发言等操作时，系统将向其他与会者以及主持人播报。

System Configurator—This module prompts you to fill in all pertinent set-up information to initiate motion.

系统配置——这个模块提示您填写所有相关初成立信息启动议案。

Histological evaluation revealed diffuse collagen deposition with patchy areas of more confluent scarring and associated alveolar contraction.

组织学评估提示，弥散的胶原沉积伴有较多的融合瘢痕的斑片状区域及相关肺泡收缩。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！