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DNA extraction ; Gut microflora ; Denaturant gradient gel electrophoresis

DNA提取;肠道菌群;变性剂梯度凝胶电泳

METHODS:Using Dual-wavelength TLC-scanning,the sample was prepared through the column full of D101 macroporous resin with n-butanol extract from the aque ous solution of the granules which had been washed by ether,developer is chloroform-methanol-water(13∶10∶7)(stored at less than 10℃,the lower),detecter is 510nm,reference is 700nm.

双波长薄层扫描法,经乙醚洗涤、正丁醇提取和D101大孔吸附树脂柱层析法制备样品,以氯仿-甲醇-水(13∶7∶2)下层液为展开剂,检测波长为510nm,参比波长为700nm。

Soaps are emulsify ing agents commonly used for cleaning; they have long been made from lye and fat.

肥皂是一种乳化剂,常被用于洗涤,一直以来是从碱液和脂肪被提取出来。

In this paper, ethanol is choosed as the entrainer to the solanesol supercritical CO2 extraction experiments by Soxhlet extraction.

本课题采用索式提取方法,确定了乙醇为超临界CO2萃取茄尼醇的夹带剂。

Methods The effects of pressure, temperature, time, CO2 flow rate types, and volume of entrainer on the urosolic acid extracts were studied. The optium conditions for SFE CO2 was determined. The technology of SFE CO2 was compared with that of traditional solvent extraction.

探讨萃取压力、萃取温度、萃取时间、CO2流量、夹带剂类型及用量对熊果酸收率的影响,确定了超临界CO2萃取山茱萸中熊果酸的适宜条件,并将超临界CO2萃取法与传统的有机溶剂提取法进行了比较。

Using water distilled the Dark Brown Pigment from Walnut epicarp in the sample of differend sampling point and same means dry .

对不同地方采集的核桃外果皮用同样的方法进行干燥后,以水为萃取剂,提取棕褐色色素并对其稳定性进行了测试和比较。

Objective In order to search for new tumor cell differentiation inducer, the antitumor effects of the tadpole ether extract (T8712) were studied.

目的 为了寻找新的肿瘤细胞分化诱导剂,探讨蝌蚪醚提取液(T8712)的抗肿瘤作用。

Methods The effective components of Eugenia caryophyllata Thunb were extracted by steam distillation and water - extraction methods.

方法应用蒸馏法及水提法提取丁香中有效成分,采用分散法制备凝胶剂;以红霉素软膏为对照品,临床观察该制剂治疗口唇疱疹的疗效。

Under the guidance of the principle of independence and self-reliance, thesubstituted amide-type A-101 extractant has been successfully synthesized.

本文叙述了在独立自主、自力更生的原则指引下,研制成功酰胺型萃取剂,以及应用于稀有金属的提取和分离的许多有成效的示例。

Methods:The Chitosan was used for the clarification of the extraction,and the flocculation conditon was studied.

本文以壳聚糖作为絮凝剂对提取液进行澄清处理,并对絮凝条件进行优化。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?