提取

Results:The best extraction technology conditions were as follows: the material was pulverized, refluxed three times of one hour each, and 95% ethanol aqueou...

结果：蔓荆子药材粗粉，用20倍量的95％乙醇作提取溶剂，回流提取3次，每次1 h为最佳工艺。结论：优选出的工艺稳定，方法可行。

The DNA of Yak PrP expressed plasmid extracted from the recombinant expressed bacterium was identified by PCR amplification and two-ezyme digestion. The expressed product was obtained from the recombinant bacterium inducted under the optimized expressed condition (37℃, 1 mmol/L IPTG, 6 h), and it was determined by SDS-PAGE and Western blotting. The GST-BoPrP(23—242) fusion proteins were collected by two ways: the first is to purify and renature from the inclusion bodies; the second is to separate by SDS-PAGE.

将保存的含有牦牛PrP基因重组表达菌提取质粒DNA，进行PCR扩增和双酶切鉴定，并在优化诱导表达条件(37℃，1mmol/L IPTG，6h)下，获得的表达产物进行SDS-PAGE和Western blotting检测；将鉴定之后的重组表达菌诱导表达后，通过两种方法回收GST-BoPrP(23～242)融合蛋白：其一，是从包涵体中提取和复性GST-BoPrP(23～242)融合蛋白；其二，是通过SDS-PAGE直接分离并纯化GST-BoPrP(23～242)融合蛋白。

METHODS: Total RNA and protein were extracted by Trizol and RIPA Buffer from heart, brain, kidney, skeletal muscle and spleen tissues of C57BL/6 and BALB/c mice.

取C57BL/6和BALB/c小鼠心脏、大脑、肾脏、骨骼肌和脾脏组织，以Trizol提取总RNA， RIPA Buffer提取组织总蛋白。

Methods: The three lung cancer cell lines-A549(highly invasive human lung adenocarcinoma cell ), 95-D(highly metastatic human lung carcinoma cell), and SPC-A-1(lowly metastatic human lung adenocarcinoma cell) were cultured conventionally in vitro. When the cells covered the bottom of culture flask, we abstracted the protein with lysis solution containing RIPA and PMSF, and obtained RNA with RNeasy Mini Kit.

将三株肺癌细胞：高侵袭的肺腺癌细胞(A549)、高转移的肺癌细胞(95-D)、低转移的肺腺癌细胞(SPC-A-1)做常规细胞培养，待细胞长满瓶底后，用RIPA和PMSF组成的细胞裂解液提取细胞蛋白质，RNeasy Mini Ki提取RNA。

Roentgenographic and histologic observation confirmed that bBMP had efficient bone morphogenetic activity.

论文第一部分参照杨连甲的方法从小牛骨基质中提取bBMP，经SDS-PAGE电泳分析，bBMP保留有BMP活性蛋白成分，植入小鼠股部肌肉后的X线及组织学观察发现，所提取的bBMP具有较高的骨诱导活性，诱导成骨的时间和过程与以往的报道一致。bBMP诱导骨形成过程呈现连续又具显著的阶段性，本研究结合组织学和组织化学观察的特点，将其全过程分为四个时期。

Firstly, we processed hierarchy structure partition to the video, and analyzed the necessity of events as the retrieval units from the point of rogatory granularity in video retrieval; Secondly, from the video editing, we proposed a new technique that extracted the events based on the representational shots in order to low the computational complexity and prepare for the last work.

首先对视频进行了层次化的结构划分，从视频检索的查询粒度角度分析了以事件作为检索单元的必要性；其次从视频编辑特点出发，利用了基于代表性镜头的镜头组聚类手段，通过对视频数据进行聚类提取事件进而为提交片段查询作好准备，此法节省了在事件提取过程中的计算量。

One-step extraction with polar and nonpolar mixed solvents was a method of effective comprehensive extraction for antioxidant and essential oil of rosemary (Rosmarinus officinalis L.). The method was simple on operation and was easily put into practice.

极性和非极性混合溶剂一步提取法是迷迭香抗氧化剂和精油综合提取的有效方法，操作简单，容易实施。

Result In PDA culture medium to add 10%-20%, 20%-30%, 20%-40% of the Tenebrio molitor Linnaeus extracts, the mycelium of Russula, Coprinus comatus, Pleurotus and Pleurotus geesteranus growth had increased markedly, but the mycelium of Hericium erinaceus had not significantly growth, and when the Tenebrio molitor Linnaeus extracts concentration was more than 20%, it was inhibition.

结果］在PDA培养基中加入10%~20%、20%~30%、20%~40%的黄粉虫粪提取液时分别对红菇、平菇和鸡腿菇、秀珍菇菌丝丝生长具有明显的促进作用，但对猴头菇菌丝生长影响不显著，且黄粉虫粪提取液加入量达到20%以上时出现抑制现象。

The apoptosis induced by extract of Russula subnigricans Hongo was investigated in little white rat liver and kidney cells by agarose gel electrophoresis.The result showed that agarose gel electrophoresis of DNA extracted from poisoned little white rat liver and kidney cellsrevealed typical 180 ~ 200bp integer-fold "ladder" bands.

亚稀褶黑菇提取液能诱导小白鼠肝肾细胞凋亡，亚稀褶黑菇提取液诱导肝肾细胞凋亡是亚稀褶黑菇中毒后机体病变死亡的重要表现形式之一 5。

In this paper, several methods, such as remote sensing digital image processing, airborne γ spectrum remote sensing data processing, quantitative extraction of redox transitional belt etc, are recommodated to extract the fossil stream channel sandrock uranium information.

本文探讨利用遥感数字图像处理、航空γ能谱遥感数据处理、定量提取氧化还原过渡带等方法来提取古河道型砂岩铀矿的信息，并在该区进行了验证，效果较好。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失