提取

In order to cutting down the cost, flocculation of recombined Erwinia carotovora-Eccl3B cell from broth, influence of temperature, pH and oxidants on the separation of PHB were studied; and the method which measure the molecular weight of PHB by intrinsic viscosity was also studied; furthermore, a phenomenon was found that insolubility of PHB in chloroform can be changed by pretreated at over 120℃ for the fist time, and the mechanism of this phenomenon was explored.

为了降低成本，提高生产效率，本文针对发酵法生产PHB过程中存在的，发酵液难以分离沉淀，非有机溶剂提取PHB时分子量严重降低等问题，进行了发酵液的絮凝，及温度、pH、氧化剂对提取PHB影响的研究。并对粘度法测定PHB分子量的方法进行了研究，同时，对于研究过程中发现的，加热后PHB在三氯甲烷中的溶解性变化及其原因进行了探索。

Methods The SpaA was purified by electroelution from NaOH-extracted antigen of Erysipelothrix rhusiopathiae strain C43311. The rSpaA-N was expressed in E. coli BL21 as a soluble protein by IPTG inducing,and purified with GST affinity chromatography. Mice of each group were subcutaneously immunized three times with 50μg or 100 μg of native SpaA, rSpaA-N or NaOH-extracted antigen with in complete or incomplete Freund adjuvant at 2-week intervals.

将猪丹毒丝菌C43311株SpaA-N以可溶形式表达在大肠杆菌BL21中，用GST Bind Resin纯化试剂盒纯化rSpaA-N，采用电洗脱法从猪丹毒丝菌C43311株NaOH提取抗原中纯化天然SpaA，将rSpaA-N、天然SpaA和NaOH提取抗原制成亚单位疫苗，同时设GST及生理盐水对照组，间隔2周分3次皮下免疫小鼠，第3次免疫后2周用100LD50猪丹毒丝菌C43065株进行腹腔攻毒，采用间接ELISA方法检测免疫组小鼠血清的抗体动态变化。

Under ultrasonic extraction, comparing the ratio of total alkaloid with 95% ethanal, hloroform, methanol.

用超声波提取法比较了以95%乙醇、氯仿、甲醇为溶剂的提取率，结果依次为1.21%、1.57%、1.54%。

The powder of eucommia was extracted twice with 60% ethanol-water solution at 60℃, for 60 min each time.

结果表明：杜仲粉用体积分数为60%的乙醇水溶液，60℃，提取2次，每次1 h，PDG提取率可达90%以上。

Under the guidance of DPRIF integrating strategy, we set up an integreted SOFM-SVM model. Then we analyzed and optimized the model from the aspects of operating mechanism, data interface and function expandedness. The PCA method was introduced to reduce dimensionality and extract features, then to strengthen the clustering explanation; defined a CMI index, to ascertain the most effective or the best clustering number; A new Anti-NO algorithm was proposed to recognize and to filter the suspecious data in the sample; The medium result of SVM model was used to extract the borderline datas between two classified groups. This research compensated for the achievements of data recognition including pattern data, noises data and borderline data.

在DPRIF整合策略指导下构建了一个整合的SOFM-SVM模型，对该模型从运作机制、数据接口、功能扩展几个方面进行分析和改进：引入PCA方法进行数据降维和特征提取，用以加强聚类解释；结合统计聚类中的聚类误差概念定义一个聚类数有效性指标，利用SOFM算法中间结果进行指标求解，以筛选出有效或最佳聚类数；提出一种新的噪声识别算法用于对样本中的异常数据进行甄别和过滤；利用SVM模型的中间结果提取分类边界数据；进一步充实了包括模式类、噪声集和边界在内的模式识别成果。

The research aimed to study the extraction of effective components from Nepeta pratti Lèvl by supercritical CO2, and provide the basis for exploring a best extractible method and analyzing the extracts accurately.

［目的］利用超临界CO2萃取方法提取康藏荆芥中的有效成分，为找出康藏荆芥中有效成分的最佳提取方法并对提取物进行准确分析奠定基础。

A pivotal problem to recognize of the precise position of laser facula has been solved in the process of design work: Firstly the method of background-subtraction is used to eliminate or weaken the affect of background light. Secondly the image threshold segmentation operation and morphologic operation are used to locate the sketchy position of laser facula ; Then the new method of Extracting Edge are designed based on character of laser facula , by using this method in the search area the edges of laser facula can be extracted effectively; At last the optimum picture of laser facular is selected by using the triggering function of hardware and function of software. The exact result can be obtained only by image processing to optimum picture.

在设计过程中解决了精确确定激光斑中心位置这一关键问题：首先利用减背景法，消除或减弱背景光的变化对确定激光光斑中心的影响；再对减完背景后的图片进行阈值分割和二值化处理以确定激光光斑的粗略位置；根据本文激光光斑的特征，设计了一个有效的边界提取算法，对指定区域进行提取激光光斑边界操作，精确的确定激光斑中心位置；最后利用硬件的触发功能和软件编程实现了激光光斑最佳图片的判读，只有对最佳图片进行图像处理得到结果才是准确的。

The optimization of extraction method of total flavonoids from Fagopyrum tatarium was obtained by using orthogonal test design,and the content of total flavonoids was determined from ten breed Fagopyrum esculentum Moench 'seeds.

采用正交设计实验，以荞麦中总黄酮得率为考察指标，对影响荞麦总黄酮提取率的因素进行了探讨，得出了荞麦总黄酮提取的优化条件，并测定了10种荞麦种子总黄酮的含量。

The extraction condition of gardenia pigment from gardenin fruit and dyeing condition of wool fiber were defined.

优选了栀子色素的提取条件和提取液对羊毛纤维的染色条件。

The process of extracting flavonoids from the residue of glycyrrhiza was introduced and the conditions of extraction were studied by orthogonal design in this paper.

本文研究了甘草渣中总黄酮的提取工艺，用正交分析法，找出了热回流法最佳的提取工艺条件，并经过放大试验，得到很好的效果。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。