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In order to isolar high quality DNA from Trichosanthes kirilowii Maxim leaves enriching polysaccharides and polyphenols, the effects of DNA extraction were studied with four different methods including CTAB regular method, CTAB subarea method, SDS regular method, Low pH extraction medium with high salts.

为了从富含多糖和多酚等次生代谢物质的栝楼叶片中分离出高质量的基因组DNA,以栝楼叶片为材料,对4种不同的DNA提取方法(CRAB常规法、CTAB区室法、SDS常规法、高盐低pH法)进行了试验研究,并首次采用CTAB区室法提取栝楼基因组DNA。

The method is simple,convenient,and this extraction procedure is helpful for further study of extraction technology of triterpene acids of Herba euphorbiae Humifusae.

提取方法操作简单、方便,为地锦草槲皮素提取工艺的进一步研究提供了依据。

The optimum conditions were achieved and demonstrated as follows: ethanol solution (volume fraction as 60 %) as extraction solution, the ratio of materials to solution as 1:20, microwave power as 600 W and 2 mm extraction time. By the optimum conditions, the yield (83.7%) of flavone from Trollius chinensis Bunge was obtained at 83.7%.

实验结果表明:微波辅助提取金莲花黄酮的最佳工艺条件是体积分数60%乙醇为溶剂,每克料浸提剂体积为20mL,微波功率600W,温度60℃,提取时间2min。

Supplemented with 5% coconut milk or 0.2% tryptone in the medium markedly promoted the hypocotyl callus growth, while 0.2% yeast extract had no important effect. Supplemented with 10% coconut milk, 2% tryptone or 2% yeast extract all inhibited the hypocotyl callus growth.

添加5%椰子乳和0.2%胰蛋白胨显著地促进生长,添加0.2%酵母提取液则未表现出显著的促进作用,而10%椰子乳、2%胰蛋白胨和2%酵母提取液均抑制愈伤组织的生长。

The size of round bottom flask, temperature and water addition were the key of volatile oil extraction of C. uncinata, and addition of N-hexane was helpful for the extraction and collection of volatile oil.

圆底烧瓶的大小、温度及加水量是柱果铁线莲挥发油提取的关键,而添加正己烷有利于挥发油的提取与收集。

In this paper,an improved genetic algorithm is proposed to overcome the shortage of early convergence and stagnation in the traditional genetic algorithm,which is based on unconvertible rate of crossover operator and mutation operator.Moreover,the user page interest measure threshold is introduced into the association rules mining.

针对传统遗传算法在提取关联规则问题时常采用固定染色体交叉概率和染色体变异概率,容易出现早熟、收敛速度较慢的问题,提出了改进的遗传算法,并在关联规则的提取中增加了用户页面兴趣度这一阈值,成功地运用到某商业网站服务器日志挖掘。

This article brings forward the technology and method of extracting xylose from the black corncon by natural zymotechnics and hydrolyzati unden the condition of diluted acid and ordinary pressure.

提出了用自然发酵和稀酸常压水解法从黑玉米芯中提取木糖的工艺和方法,通过红外光谱和紫外光谱确证结构,其产率达到28.65%,并讨论了影响木糖提取率的因素。

The crucial procedure of contour detection for microscopic image of sprayed droplets and measurement of granularity is to segment droplets from background by producing binary images. The singular threshold is uneffective because the image is very different in illumination. In this paper, a fast maximum entropy image thresholding algorithm based on local gray feature is presented to exact sprayed droplets from background and preserve contours of droplets.

雾滴图像的颗粒提取与测量关键是分割出雾滴与背景,生成二值图像,由于雾滴图像光照不均匀,用单一阈值无法兼顾亮区与暗区,本文提出了一种基于局部灰度特性快速最大熵动态阈值选择算法分割出雾滴微粒并提取雾滴轮廓。

The infrared image has blur edges and many noise points. Traditional methods need to calculate the grads, so they are sensitive to unregular details and noises. When the contrast is low, departure may appear for extracted transition region, thus will affect the judgement of segmentation threshold value and the segmentation result.

红外图像边缘模糊、噪声点多,而传统的过渡区提取算法需计算梯度因而对非规则细节和噪声敏感,尤其当对比度较低时极易导致提取的过渡区发生偏离,影响分割阈值的判定和分割结果的准确性。

Steeped corn endosperm and unsoaked corn endosperm were used respectively for zein extraction. The results indicated that steeping was helpful for the extraction.

分别用浸泡过的整粒玉米和胚乳及未经浸泡的胚乳提取玉米醇溶蛋白,结果表明,浸泡有利于醇溶蛋白的提取

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。