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Based on it,the extraction efficiency of sinapine were compared between microwave-assisted extraction ultrasonic-assisted extraction and hot alcohol extraction .

方法采用正交实验,考察提取时间、提取温度、料液比3个因素的影响,建立芥子碱热醇提取的最佳提取条件。

During the extracting process, three main factors including ratio of solid to liquid,ultrasound power and ultrasound extracting time were studied by using single factor analysis method .

提取过程中,通过单因素法分析了几个主要因素:料液质量比、超声波提取功率及超声波提取时间对提取率的影响。

Regarding the content of total sapnins in Snap Bean as the index of investigating, the factors affected extractive content of the sapnins from snap bean was studied.

研究了提取溶剂浓度、提取温度、固液比、提取时间、提取次数等因素对总皂苷含量的影响。

The result showed that the highest extraction rate of polysaccharides from Sophora japonica could be obtained with a ratio of solid to liquid as 1:25, 80℃ for 6 h and 4 times of extraction.

提取槐花多糖的工艺条件为:料液比1:25,提取温度80℃,提取时间6 h,提取次数4次。

Draw the effective composition of the red red-spotted stonecrop through drawing the law in ultrasonic wave at first, learn through the experiment that drawing time , density , firm liquid in supersound will have remarkable influence than on the drawing rate of supersound; 60% ethanol solution of solution, than remarkable ultrasonic reciprocation of time firm liquid have; The best experiment condition is: Solvent solution, firm liquid than for 1:10, draw 45min for 60% ethanol..

首先通过超声波提取法,来提取红景天的有效成分,通过实验得知超声提取时间、浓度、固液比对超声提取率均有显著的影响;溶液中的60%乙醇溶液,固液比及超声时间的交互作用显著;最佳实验条件为:溶剂为60%乙醇溶液、固液比为1:10、提取45min。。

Analysis indicated that the ratios of the Cd forms extracted by ethanol and by deionized water in root, and the ratios of soluble Cd in root cell showed accordancy with the ratio of Cd transported from root to aboveground parts, and also the ratios of the Cd forms extracted by ethanol and by deionized water in stem, and the ratios of soluble Cd in stem cell showed accordancy with the ratio of Cd translocated from rice straw to grain.

分析表明,两供试品种根组织中乙醇提取态和去离子水提取态Cd的比例及根细胞中可溶性Cd的比例与根系吸收的Cd向地上部的分配比例存在一致性;茎组织中乙醇提取态和去离子水提取态Cd的比例及茎细胞中可溶性Cd的比例与植株地上部积累的Cd向籽粒分配的比例间存在一致性。

The steam distillation. 2.Rotary evaporation distillation, 3.supercritical extraction for the extraction of volatile oil oriental arborvitae, the experiment results showed, 1.the best conditions for the extraction by rotary evaporation are NG:water:ethanol=l:4:4 sawdust under the conditions 50 ° C in temperature, atmospheric pressure 0.1 Pa, extract maximum extraction rate of 90 minutes.

对不同方法提取得到的侧柏挥发性物质的粗提物进行了的触角电位测定和行为测定,确定了利用水蒸气蒸馏、旋转蒸发蒸馏、超临界萃取三种方法进行侧柏挥发油的提取,并通过试验得到旋转蒸发仪萃取的最佳方法为:在木屑:水:乙醇=1:4:4;50℃温度;0.1个大气压的条件下,提取90min的提取率最高。

Isolations of the s.aureus insoluble peptidoglycan:(1) the s.aureus insoluble peptidoglycan was gained by cold, hot and hypersound affection repetat of s.aureus(ATCC25923);(2) the s.aureus insoluble peptidoglycan was gained by 10% trichloracetic acid, 5% trichloracetic acid,NH4HCO3 buffer with trypsin digestion of s.aureus(ATCC25923) in order. 2. Isolations of the s.aureus soluble peptidoglycan and quality control:(1) the s.aureus soluble peptidoglycan was released by short dose benzylpenicillin sodium and purified by sephadex G-100.(2) The quality control in the process of isolation: the soluble peptidoglycan was detected by SLP reagent kit; the LPS was detected by kinetic turbidimetric limulus test;and the protein was detected by improved LOWRY reagent kit.

金黄色葡萄球菌可溶性肽聚糖的分离提取及其质量控制(1)采用青霉素钠诱导法和葡聚糖G-100(Sephadex G-100)凝胶柱分离提取金黄色葡萄球菌可溶性肽聚糖;(2)金黄色葡萄球菌可溶性肽聚糖提取过程中的质量控制:采用SLP试剂盒对提取物定性检测,应用动态浊度法鲎试验测定提取物中内毒素含量,采用改良LOWRY试剂检测提取物中蛋白的含量;(3)采用青霉素诱导和Sephadex G-100凝胶柱分离提取金黄色葡萄球菌可溶性肽聚糖的条件:青霉素使用量、加入青霉素后孵育时间和菌液浓度等优化选择。

Results indicate that the optimum extraction conditions are as follows: ultrasonic power is 600W, temperature is 53℃, the ratio of liquid to solid is 8︰1, ultrasonic treating time is 70min, reflux extraction time is 120min, the extraction rate of oleanolic acid is 3.000 mg/g; XDA-1 resin is favorable in purification of OA in Chaenomeles sinensis Koehne, the optimum purification conditions are found to be 0.794 mg/mL of OA concentration, the flow rate of adsorption is 0.5 mL/min; the desorption solvent is 90% ethanol, and the flow rate of elutant is 1.0 mL/min.

结果表明,超声波辅助提取最佳工艺条件为:超声功率600 W、提取温度53℃、液料比8:1、超声波提取70 min,回流提取120 min,齐墩果酸得率为3.000 mg/g;XDA-1型大孔树脂对齐墩果酸具有较好的吸附和解吸效果,最佳纯化工艺条件为上柱液浓度0.794 mg/mL,吸附流速0.5 mL/min;以90%浓度乙醇洗脱,洗脱流速1.0 mL/min,纯化后得提取物中齐墩果酸纯度可达26.55%。

Result The optimal condition was as follow: Raw materials was 0.500 g Chimonanthus praecox Link. dry powder, the extraction agent was 70% alcohol solution, the ratio of raw material to extraction agent was 1:60, the microwave power was 288W, and the extraction time was 450 s for three times.

结果]原料0.500 g的腊梅花粉末,以70%的乙醇溶液作提取剂,提取剂的用量为1:60,微波功率为288 W,在提取时间为450 s下提取次数3为最佳工艺。

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