接种后的

Coli mainly in a form of inclusion body. The expressed product contained about 25% of total somatic protein and showed good reactogenicity as proved by Western blot. The IgG level against GAMP induced by GAMP-CRM197 conjugate was significantly higher than those by GAMP and by the mixture of CAMP and rCRM197. Repeated immunization with the conjugate induced immunopotentiation, indicating the effect of CRM197 as a carrier protein.

结果重组CRM197在大肠杆菌中主要以包涵体形式表达，表达量占菌体总蛋白的25%左右；Western blot证明重组rCRM197具有良好的反应原性；各针接种后，结合物诱生的多糖特异性IgG水平均显著高于GAMP组和GAMP+rCRM197混合物组，具有较强的免疫原性；多次接种产生了免疫增强效应，重组CRM197具有载体蛋白的作用。

In order to develop a safe and effective immunoadjuvant to enhance the immunity and resistance of animals against infection, a novel CpG Oligodeoxynucleotides containing 11 CpG motifs was synthesized and inserted into the VR1012 plasmid, designated as VR1C. Then the recombinant VR1C was entrapped with Chitosan nanoparticles prepared by the method of ionic cross linkage, and employed to inject muscularly 3-weeks old Kunming mice; the blank VR1012 packed with CNP and saline were used to inoculate mice as the control groups. 28 days after inoculation, all mice were orally fed with 0.4ml 2x108CFU/ per mouse virulent hemorrhagic enteritis E. coli to challenge the resistance against infection.

为研制安全高效免疫调节剂增强动物免疫抗病能力，本实验设计合成含11个C pG基序的寡聚核苷酸，重组构建含CpG的VR1012质粒(VR1C)；制备壳聚糖纳米颗粒包裹重组质粒(CNP-VR1C)，肌注接种3周龄昆明小白鼠，设壳聚糖包裹空质粒和生理盐水对照组；接种后28天口服大肠杆菌攻毒观察小鼠天然免疫的变化和对强毒感染的抵抗力，Sandwich ELISA测定血清免疫球蛋白和白细胞介素含量。

Histological and cellular research on rachis of healthy and inoculated spikes of the mutants at the different stages showed that, structure and developed tendency of cortical sclerenchyma tissue and green tissue and fibrovascular tissue in the rachis were alike between the mutants and their donors at anthesis stage, which also showed no apparent difference in the inoculated spikes'rachis between the mutants and their donors, major difference was that the mutants had less hyphae number than their donors.

对抗赤霉突变体的健康穗及接种穗的穗轴细胞组织学研究结果显示，抗性突变体与其感病亲本的花期健康穗轴在皮层厚壁组织、绿色组织及维管组织结构上基本一致，不同发育时间的变化趋势也一致；接种处理后，抗性突变体与其感病亲本的穗轴组织在各方面的变化趋势相一致，变化量也无明显差异。

Experimental mice were exposed to Trypanosome evansi after injection by different doses of antigenic reservoir. The results showed that 20 μg dose produced 83% and 67% protection to 2 sample variations after 4 immunizations. However, 3 immunizing doses could not give good protection. No protective effects were detected when experimental animals immunized by single VSG exposed to isotype from different rabbits.

用不同剂量抗原库免疫小鼠后进行攻虫，结果显示：经四次免疫后，20μg 剂量对随机抽取的两个变异体100 条虫体的保护率分别达到 83%和 67%；改变免疫方法，经三次免疫后的保护效果不理想；用单一 VSG 抗原免疫小鼠，接种来自不同兔体的同型虫体，不能起到相应保护作用。

Methods The recorded information of 45 orthopedic surgeons who have encountered the occupational exposure to blood-derived pathogen (i.e.occupational exposure) have been accumulated from Jan 1st 2004 to Jun 30th 2006.The condition of the accident,category of the acumination,hepatitis B vaccine inoculation before the occupational exposure,the disposal after the hurt and the follow-up after the acumination hurt were analyzed.

方法收集从2004年1月1日-2006年6月30日所有发生血源性病原体职业暴露的骨外科医生登记资料45例，对职业暴露者暴露源血源性传播性疾病情况；锐器种类；职业暴露前乙肝疫苗接种情况；锐器伤害后的处理措施；锐器伤害后追综观察结果等进行分析。

METHODS: Bone of limbs were obtained under sterile condition, washed by PBS, centrifugalization, and dropwise into centrifuge tube containing 1.073 g/mL Percoll separating medium, centrifugalization, and then adding L-DMEM containing 10% fetal bovine serum, benzylpenicillin sodium and phytomycin, cultured in a incubator at 37 ℃ with 5% saturated humidity. The culture medium was renewed after 48 hours, and non-adhesive cells were removed.

无菌条件下截取流产胎儿双侧四肢骨，PBS冲洗髓腔，离心去脂肪及上清液，L-DMEM重悬细胞，沿管壁缓慢滴加至底部有等量1.073 g/mL Percoll分离液的离心管中，离心后吸取中间界面白膜层，加入含体积分数为10%的胎牛血清、青霉素钠、链霉素的L-DMEM完全培养基，接种后置于37 ℃、体积分数为5%的CO2饱和湿度孵箱内培养。48 h后换液，去除未贴壁细胞，以后每2 d换液一次。

The puerperant and her relatives all signed informed consents. METHODS: Bone of limbs were obtained under sterile condition, washed by PBS, centrifugalization, and dropwise into centrifuge tube containing 1.073 g/mL Percoll separating medium, centrifugalization, and then adding L-DMEM containing 10% fetal bovine serum, benzylpenicillin sodium and phytomycin, cultured in a incubator at 37 ℃ with 5% saturated humidity.

无菌条件下截取流产胎儿双侧四肢骨，PBS冲洗髓腔，离心去脂肪及上清液，L-DMEM重悬细胞，沿管壁缓慢滴加至底部有等量1.073 g/mL Percoll分离液的离心管中，离心后吸取中间界面白膜层，加入含体积分数为10%的胎牛血清、青霉素钠、链霉素的L-DMEM完全培养基，接种后置于37 ℃、体积分数为5%的CO2饱和湿度孵箱内培养。48 h后换液，去除未贴壁细胞，以后每2 d换液一次。

The results showed that Acidithiobacillus ferrooxidans could effectively solubilise realgar and 16.3% arsenic dissolution could be achieved in 30 days under the conditions of pH 1.8 in Iron free 9 K medium (with additional 1.0g of ferrous iron per liter and 0.5% of realgar), 150 rpm, 30°C. The trace elements of Zn, Cu, Mg, Mn, Fe and Se et al. could also be effectively leached.

实验结果表明，氧化亚铁硫杆菌能够有效溶解雄黄中不溶性的硫化砷，30 ℃，pH1.8，以20%接种量接种驯化后的氧化亚铁硫杆菌BY-3条件下135r/min摇瓶浸出30天砷的溶出率可达16.3%，Zn、Cu、Mg、Mn、Fe、Se等有益微量元素都能够得到有效浸出。

The positive seroconversion rates of neutralizing antibody were 100%(n=27 GMT 2.89 IU/ml) and 100%(n=30, GMT 5.29 IU/ml) respectively by pre-exposure and post-exposure schedules.

结果 全部受试者仅显示12%的轻度副反应，无中强度副反应；不管以暴露前或暴露后方案接种，全程注射后的中和抗体阳转率均为100%。

Null mutant Δmnh6 produced appeared offwhite hyphae, showed reduction in growth, conidiation and appressorium formation, and also showed reduction in appressorium turgor pressure. And the hyphae were more readily wettable by a solution containing SDS and EDTA and more easily digested by fungal cell-wall-digesting enzymes. The appressoria produced by Δmnh6 mutants showed diminished host penetration and the infectious hyphae of Δmnh6 mutants showed reduced in planta growth. Targeted deletion of mnh6 resulted in the greatly reduced virulence capacity to barley and rice CO39 in infection assays.

突变子Δmnh6的菌落形态不同于野生型GUY11，菌落呈灰白色，菌丝稀疏，生长速度变慢，产孢菌丝细胞壁的溶剂疏水性消失，菌丝细胞壁容易被细胞壁降解酶降解，产孢减少，孢子形态变得细长，孢子的附着胞形成率降低，附着胞的膨压降低，附着胞对寄主植物表面的穿透能力减弱，侵入后生长速度减慢，喷雾接种后对大麦和水稻CO39的致病性消失。mnh6基因重新引入Δmnh6后，突变子的表型恢复。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。