接种后的

Through all this test analyse Pathogenicity and Immunological status of ALV-J Inner Mongolia strain, the result showed the infected chickens began to have pathological change of Myelcytomatosis at 3-week: the main pathological changes of acidophile myelocyte were found in bone marrow and between cardiac muscle fibers,and there were many granules in the hyalotome.This kind of oncocyte proliferated in every tissue at different amount, but the cells that proliferated in bone marrow, cardiac muscle fiber, oarium were much more than other tissue, and they often developed neoplasma. At same condition,there were no abnormalty advent in control group, In immunological aspect: the increase of lymphokine and ANAE positive cells showed cell-mediated immunity was strengthend during antineoplastic infection; the emergence of ALV-J antibody of ELISA suggest there was humoral immunity exist at the same time.

结果显示：脾脏PCR检测结果表明1、2两实验组均有阳性病例出现，而对照组全部为阴性，说明实验鸡体内已有ALV-J内蒙株感染；接种病毒后3周龄开始出现骨髓细胞瘤病的病理变化：肝脏、脾脏有程度不同的肿大，镜下观察，骨髓内最早出现了与骨髓细胞相似、形态基本一致的大型圆形或椭圆形、胞核较大多为椭圆形、胞浆内含有大量嗜酸性圆形颗粒的髓细胞样瘤细胞增生，随后通过血流瘤细胞可转移到肝脏、心肌、卵巢或睾丸等组织并形成肿瘤性病灶，而对照组没有出现明显病变；免疫状态方面：以脾脏淋巴因子和ANAE阳性细胞增多说明细胞免疫在抗肿瘤感染过程中增强，ELISA检验实验鸡抗体出现阳性的结果表明同时也存在着体液免疫。

The result showed that the best time for sampling was 7 to 14 days after the first bud flowering on the main anthotaxy. The capacity of embryoid production varied significantly in different genotypes. Among them, twelve genotypes yield embryos successfully, which the rate could reach 71%. Genotype 102 was an ideal material for embryoid induction, and the frequency could reach 32.78 embryos per bud. Genotype that with a highest yield produced 546 times as many embryos as that with a lowest yield. The embryoid induction ability of normal bolting genotypes were higher than easy bolting and hard bolting genotypes.

结果表明：在秋冬季日光温室内，供试植株主花序第一朵花开后第7~14天取样，即初花期是最佳取样时期；不同基因型间小孢子胚胎发生能力差异很大，在接种的17个基因型中，有12个诱导出胚，诱导成功率71%；其中102号产胚量最高，达到32.78个／蕾；在已产胚材料中，产胚量最高的基因型是产胚量最低的基因型的546倍；较易抽薹型材料的产胚能力大于易抽薹型和不易抽薹型。

And the culturable bacterial degraders were increased from 10^6CFUg^(-1) soil to 10^8CFUg^(-1) soil after inoculating with the degrader. Moreover, the bacterial population increased faster in 100mgg^(-1) microcosoms than in 500mgg^(-1) microcosoms, revealing that the inoculated bacteria could survive and enhance the biodegradation of chlorophenol in cuntaminated soil.

接种外来微生物后，土壤中可以培养的氯酚降解菌的总数从开始的10^6CFUg^(-1)增加到10^8CFUg^(-1)，并且，在氯酚含量为100mgg^(-1)的土壤样品中，微生物数目的增加比在氯酚含量为500mgg^(-1)土壤样品中更快。

In order to study the digestion of fibrous materials ADF and NDF,the alfalfa,ryegrass and their powder mixture were chosen as the substrates,and mixed with the cecum chyme filtrate for fermentation.pH value declined to 6.9 during first three hours fermentation,and then came to be stable.

以苜蓿草粉、黑麦草粉和混合草粉(质量比为1∶1)为底物，接种鹅盲肠食糜滤液进行体外培养。试验结果为：培养液pH值在0~3 h下降到6.9左右，后逐渐趋于稳定；苜蓿草粉ADF、NDF和半纤维素的消失率分别为1.15%，1.18%，-0.19%；黑麦草粉三者的消失率分别为1.45%，9.05%，3.07%；而混合草粉三者的消失率分别为15.72%，13.2%，9.87%。与单一草粉的ADF、NDF和半纤维素的消失率都有显著差异(P.05)。

The tests have Simulated the rainstorm state and the order state, and the results indicated the disposal of microorganisms-aquatic plant system is efficient. The optimization removal rate time was ascertained under the static state test, in dynamic test, comparing and analyzing the effect on removal rates of different concentration wastewater. The results showed, without aeration, the removal rate of CODCr, TN was 85.9%,75.1%, respectively, the highest removal rate of TP was 64.6%, and the concentration of DO increased steadily from 2mg/L to 7mg/L during experiment. In dynamic test, the microorganisms-aquatic plant systems have also appeared better effect on freshet, and the removal rate of TN,TP and CODCr, was 64%,69% and 81%, respectively.

利用接种高效微生物菌剂促进填料快速挂膜，并和水生植物联合作用去除水体中的氮磷等污染物，在静态水体下研究达到最佳去除效率的时间，并在连续流试验下对比分析不同浓度下的去除效率，根据静态模拟试验结果表明，在不曝气，自然复氧条件下，CODCr和TN的最终去除率分别为84.3%和71.4%，TP最高去除率为62.9%，DO也从2mg/L稳定的升高到7mg/L左右；在连续流模拟试验结果表明，该系统对雨后入湖的河水中TN、TP及CODcr的去除率分别稳定在近64%，69%和81％以上。

Through experiment on the nutrient contribution in fish diet,thefeeding custom of dolphins was concluded,that was in winter,dolphinslike to eat fish with high energy,such as houttuyn;in summer,dolphinsturn to like fish with low energy,such as ribbonfish and polynemidae.Based on it,scientific feeding system and other relatively husbandrymethods were preliminary formed.

2通过对海豚的每一批饵料鱼体营养成分的测定分析(粗蛋白、粗脂肪、灰分、水分和总细菌数)，进而通过对海豚的食性、日食量及食前反应、食后反应等方面的研究，发现了海豚的季节性饮食喜好规律，即海豚在冬天喜欢采食鲐鱼等高能量型鱼类，在夏天喜欢采食带鱼等低能量型鱼类，首次建立了海豚的饵料投喂、营养补充物质的适量添加及定期进行驱虫、接种疫苗和体格检查等科学饲养管理方法体系。

Effects of gamma irradiation on shoot regeneration from leaf and bud growth in vitro of strawberry 1 The leaf was very sensitive to the gamma irradiation, and 'Toyonoka' was more sensitive than 'Akihime'. The LD50 (50% decrease of generation) of strawberry leaf was between 10 and 20 Gy. 2 Strawberry leaves were inoculated in vitro at 8 d-15 d after gamma rays irradiation, and the shoot regeneration rate from of 'Toyonoka' increased at 10 Gy and almost no difference from 20 Gy to 40 Gy. The positive effect dose for 'Akihime'.was from 10 Gy to 30 Gy, however, 40 Gy decreased shoot regeneration rate.

2.60Co γ辐射对不同品种草莓离体叶片再生及芽生长的影响 1）叶片对辐射敏感，叶片再生的半致死剂量为10 Gy-20 Gy，'丰香'品种比'章姬'品种敏感； 2）辐射后8 d-15 d接种，'丰香'叶片的不定芽再生率在剂量10 Gy呈上升趋势，而其他剂量则几乎未见有变化，'章姬'在剂量10 Gy-30 Gy，叶片的不定芽再生率提高较大，40 Gy仍表现轻微下降； 3）适宜的辐射剂量对草莓试管苗增殖有促进作用。

According to the bactericidal assay of quantitative vehicle, the bacteria on the ozonized vehicles and unozonized vehicles was washed, 50μl eluant was seeded on the TSA plates.

用碘滴定法测定打开臭氧发生器不同机组及不同时间时的臭氧浓度，按载体定量杀菌试验，将经臭氧作用的载体与相同环境下未经臭氧作用的载体上的细菌洗下，各取洗脱液50μl，接种于TSA平板并置厌氧箱（90％N2，10％CO2，37℃）培养24-48h后，进行活菌菌落计数。

Immunohistochemical identification of cultured conjunctival cellsWhen the second cultured generation of conjunctival cells close to confluence, the glass coverslips were removed in situ fixation by methanol and DAB color immunohistochemical staining by CK13 monoclonal antibody in time.3. growth curve of conjunctival cellsThe 1st,3rd,5th passage cells were selectecd randomly and subcultured at the density of 5×10~4/ml,after culturing for another 1-9 days, were counted and compared the cell number every day.

采用消化后组织块法培养结膜原代细胞，并进行传代。2、培养结膜细胞的免疫组化鉴定把第2代的结膜细胞接种于置在培养皿中的盖玻片上，当细胞接近融合时，及时取出作原位甲醇固定，CK13单抗、DAB显色免疫组化染色。3、结膜细胞生长曲线的测定随机选取第1、3、5传代细胞，以5×10~4／ml密度传代、分别培养1-9天，每天分别细胞计数并进行分析比较，重复3次。

Methods Cells were maintained in DMEM medium with 10% fetal bovine serum. Five days before the beginning of experiments, the cells were seeded in phenol red-free DMEM medium containing 5% charcoal dextran-treated FBS. The cells were harvested, and seeded in 6-well culture plates or in 75ml flacks. After NP, BisA and DBP treatments for 72h, the cells were harvested, and mRNA and protein expression of PCNA, bcl-2 and bax were detected by reverse transcription-polymerase chain reaction and immunohistochemistry, respectively.

方法］T47D细胞在含10%胎牛血清的DMEM培养液中进行常规传代培养，实验前将细胞转移至无酚红DMEM（含5%活性碳葡聚糖苷处理过的FBS）中继续培养5d，收集细胞，PBS洗涤后接种於内置盖玻片的6孔板或75ml培养瓶中，用32×10^(-7)mol/L NP、32×10^(-7)mol/L BisA及32×10^(-6)mol/L DBP对细胞分别处理72h，用半定量RT-PCR技术观察这3种化合物对核增殖抗原PCNA、bcl-2及bax mRNA表达的影响，并用免疫组化方法对结果进行验证。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。