接种后的

When heterotrophic bacteria were washed out while autotrophic bacteria were accumulated, the sludge mainly was bacilliform floccule, but the sludge was petallike when the steady nitrification was set up and part nitrogen removal function was reached. The lack of organic carbon source made autotrophic bacterium become the dominates and the microbial community structures become simple.

经过成功启动并稳定运行后，单级自养脱氮系统内随着反应器内有机碳源的减少，系统中自养细菌在同异养细菌的竞争中逐渐占据优势地位，适应能力较弱或对有机碳源量要求较高的异养细菌则被大量淘汰，微生物群落结构与接种污泥相比已变得简单且较稳定，种群多样性降低。

And there are no significant differences between the two sites. The human preadipocye is composited with scaffold material, and then it implants in nude mice subcutaneously. The new fat tissue also can be observed, but it is sheet, not lumpish or hemispheroidal. We still do more research in the field of growth and control of cell, screening better scaffole material in future study.

两个部位无显著性差异，将人的前体脂肪细胞与支架材料复合后接种裸鼠皮下，也能形成新脂肪组织，但为片状，不能形成团块状或半球状，今后还将对细胞的生长调控、更好的筛选成形支架材料等进行深入研究。

Study on the activity of PAL、POD and SOD in the corn after inoculated pathogen showed that there were big differences between resistance and susceptible materials. The changs were more stabilization in resistance materials than in susceptible materials. There is a wave crest of activities in resistance materials, but no activity wave crest in the susceptible.

通过对抗、感品种接种病原菌后，其苯丙氨酸解氨酶、过氧化物酶和超氧化物歧化酶活性变化的研究表明，抗、感材料之间的变化大不相同，抗病材料中的各种酶活性变化都比较稳定，并且有活性波峰；而感病材料中各种酶活性变化都比较大，并且没有活性波峰。

Cells of successive transfer culture were seeded by 5×104/ml and cultured in 24 well cell culture cluster. After confluenced, the extracts of dipsacus asperoids was applied to cell culture, then the ALP content in the culture fluid was detected.

将传代的细胞制成密度为5×10~4/ml的细胞悬液，接种于24孔培养板中，细胞融合单层后用续断甙培养液培养，测定培养液中ALP的含量。

MSCs were cultured in a conditional medium in subculture including: dexamethasone, vitamin C, and β-glycerophosphate. At the same time, we constructed the eukaryotic expression vector containing VEGF165gene. VEGF165 gene was transfected into MSCs by means of LipofectAMINE?2000. The stably gene expressive cells were screened with G-418 for 14 days.

在脂质体介导下将构建成功的真核表达载体pcDNA3.1- VEGF165转染到诱导培养的MSCs，G-418筛选阳性细胞克隆，将阳性克隆细胞和未转染的MSCs共同接种于β-磷酸三钙上，体外培养7天后，植入原大鼠肌肉内。

Methods The logarithmically growing CEM cells cultured in vitro ( cell density 2 × 105/mL) were exposed to 0.1, 0.5, 1, 5, and 10 μM Dex, then were collected 24, 48 and 72 hrs later. Untreated CEM cells were used as Controls. The cell viability was determined by trypan blue dye exclusion.

方法将在体外培养的对数生长期的CEM细胞浓度调至2×105个/mL，接种于24孔培养板中，用终浓度分别为0.1，0.5，1，5，10 μM地塞米松处理，以不加任何药物的CEM细胞作为对照组，培养后24，48，72 h取样。

Detection the early stage apoptosis cells by flow cytornetry Incubating SCL-1 cells in 6-well culture medium with 1×10~6/well, after 12～48hculture with the concentration of imiquimod 0μg/mL and 150μg/mL, gathering cellsgrowing along the wall and making monocellular suspension, washing in PBS for 2times, and then add 5μL Annexin V/FITC and 10μL PI solutions, reaction for 15min avoiding light, detection the early stage apoptosis cells by flow cytometry.

流式细胞仪测定细胞早期凋亡 SCL-1细胞以1×10~6个／孔接种于6孔培养板中，使用含0μg／mL、150μg／mL咪喹莫特的培养液培养12～48h，收集贴壁生长的细胞制成单细胞悬液，PBS洗两次，分别加入5μL Annexin V／FITC和10μL浓度20μg／mL的PI溶液，混匀后室温避光孵育15min，流式细胞仪检测，结果用Cell Quest软件分析。

Moreover, monkeys inoculated with the nef free SIV developed marked resistance to the more virulent strain.

而且，猿猴接种过已去除&否因&的SIV后，会发展出明显的反抗力，可以反抗毒性强的SIV品种。

Moreover, monkeys inoculated? with the nef free SIV developed marked resistance to the more virulent strain.

而且，猿猴接种过已去除&否因&的SIV后，会发展出明显的抵抗力，可以抵抗毒性强的SIV品种。

Thecabbagy produce by such proedure can be kept at nounal tewperation for 30 days withvacuum package, the living bimobacterium adolescentis cells of which are above 10个/g

液体菌种的接种量为2％。用此工艺制得的成品酸菜经真空包装后在常温下贮藏30天，其中的青春双歧杆菌数不少于10个／g。

By the time of its fall, most of the prisoners were writers who had written against the corruptions of the government.

到它被攻陷的时候，里面多数的犯人是写了反对政府贪污文章的作家。

The most obvious variation to ovum morphological character was that the color was changed from light green to sepiaceous in embryonic development, and all the ovums were almost hatched after 96h.

在胚胎发育过程中卵的形态特征最明显的变化是颜色从淡绿到深褐色，卵在发育96h后卵基本全部孵化。