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Well developed shoots were successfully obtained after 45 days of culture of shoot tip explants on MS medium supplemented with 4-8 mg/l Benzylaminopurine and 0.5 mg/l α-Naphthaleneacetic acid. Addition of 0.01-0.5 mg/l Thidiazuron in the medium was found to be beneficial for rapid growth of shoot tip explants.

培植体培养於MS基本盐类并添加4-8mg/l BA和0.5mg/l NAA的培养基45天后,对促进新茎节萌发效果良好;而接种於含0.01-0.5mg/l TDZ固体培养之茎顶培植体,也显示有促进茎节快速生长的作用。

After the selection from one to three generations, several mutants were obtained. The major mutants included non-nodule mutants (18 lines) which failed to nodulate after inoculated with rhizobia for two weeks, ineffective nodulation mutants (9 lines) which had less uneven and white or transparent nodules, an abnormal leaf and flower mutant which was dwarf without stipules, abnormal flowers and less nodules, and a slender mutant with a thin stem and smaller leaves.

从种植的三代植株中,筛选到多种共生固氮根瘤的突变体,其中不结根瘤突变体18个株系,表现为接种根瘤菌两周后无根瘤形成;结无效根瘤突变体9个株系,表现为根瘤数目少且分布不均匀,根瘤呈白色,有些为半透明;花叶形态异常突变体1个株系,表现为除根瘤数目少外,植株矮小、托叶消失、花形态异常;纤细突变体1个株系,表现为除根瘤数目少外,植株变小、茎细叶小。

After the lectin gene is transferred from one type of legume into another, the latter could be finally infected and nodulated by the rhizobial bacteria that originally only harbor in the former legume.

将一种豆科植物的凝集素基因转化到另一种豆科植物后,再接种前一种豆科植物的根瘤菌,可以使其被侵染和结瘤。

In group A, VX2 tumor tissue was inoculated into the rabbit kidney percutaneously under ultrasound guidance.

新西兰大白兔30只平均分为两组,A组采用超声引导下组织块注射法,B组采用手术直视下组织块接种法建立VX2肾癌模型,比较两种方法的成瘤率,种植后隔天一次监测VX2肾癌的超声声像变化。

The results of prevaccination and post-vaccination simulated by the models are very closed to the figures observed,and express that the model can simulate the transmission dynamic of hepatitis B in ...

免疫接种前、后模型模拟的结果与实际观察值相符,这说明模型能够反映乙型肝炎在人群中的传播过程。

Using this method, 105C/mL of salmonellae could be detected and it was not inter-fered by the presence of even 108C/mL of non-Salmonella bacteria.

该程序灵敏度为105个/mL,可经受108个/mL杂菌的干扰,对含有10个以上沙门氏菌的接种标本经21~23h两步增菌后即可检出,可在25~27h内报告结果,比常规分离培养方法缩短3~5d。

Result: Schwann-like cells disperse in the hole of nerve graft at oneweek under Scanning Electronic Microscope, the processes of thesatellite-like cells adhere to the wall of graft.There was no statisticaldifference of activity of cells in both group(P>0.05) using MTT. At 12weeks,the result of appearance, histology and TEM were similar, therewere no statistical difference in SFI%, NEP and quantitative analysis ofrecovery rate of myelinated fiber populations, diameter of myelinatedfiber and thickness of myelin sheat.

结果:1w后扫描电镜下可见接种的类Schwann细胞分散在材料表面的孔隙内,细胞形态为星状,细胞突起与支架管壁粘附;MTT法测定细胞活性示神经支架组在各时间点与对照组无显著性差异P>0.05;12w时两组动物坐骨神经外观、组织学光镜观察、透射电镜观察结果相似、两组坐骨神经功能指数恢复率、神经电生理、再生有髓神经纤维计数恢复率、神经纤维直径恢复率、髓鞘厚度恢复率等指标相比无显著性差异(P>0.05)。

Suspensions of field samples suspected to contain FMD virus should be clarified and inoculated into cell cultures or unweaned mice .

将可疑含FMD病毒的田间样品酊悬浮液经澄清后接种于细胞培养物或未断奶乳鼠。他们把仓库里的老鼠消灭光了。

The mice were immediately anatomized and the volume and weight of tumors were determined. Immunohistochemistry was used to determine the expression of PCNA protein and nm23-H1 protein of these cells.

接种6周后脱臼处死全部裸鼠,测量移植瘤的体积和重量,采用免疫组织化学法检测各组中增殖细胞核抗原和nm23-H1蛋白的表达。

The processing technology of Huanghua pear fermented wine are : Huanghua pear →selection, washing →cleavage breakage→pretreatment→squeezing juice →pectinase clarification(pH3.5,1%pectinase solution adding 0.15%,45℃,50minutes)→dominant fermentation (1596 yeast, temperature 20℃,inoculation volume 7%,optimum initial pH3.1~3.4,volume loading 80%,sulfur dioxide 100mg/l,circle 8 days )→seperation and taking wine →after fermentation(10~15℃,14days)→seperation and disgorging→maturation→clarification (bentonite adding 0.16%)→filtration→adjustment→bottle

9黄花梨酒的加工工艺为:黄花梨→挑选、清洗→破碎→预处理→榨汁→果胶酶澄清(pH3.5,1%酶液的加入量为0.15%,温度45℃,时间50min)→主发酵(1596菌种,发酵温度20℃,接种量7%,最适初始pH3.1~3.4,装液量80%,二氧化硫加入量100mg/l,周期8天)→分离取酒→后发酵(10~15℃,时间14天)→分离除渣→陈酿→澄清(皂土加入量为0.16%)→过滤→调配→装瓶

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The split between the two groups can hardly be papered over.

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